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101.
AIM: To study the effects of baicalin (BC) on glial fibrillary acidic protein (GFAP) and nuclear factor-κB (NF-κB) expression and neuronal apoptosis in juvenile rat hippocampus after status convulsion (SC). METHODS: One hundred and ninety five juvenile male Sprague-Dawley rats were randomly divided into 3 groups: normal saline pretreatment group (NS group), SC group and SC with BC pretreatment group (BC group). Each of these 3 groups would be subdivided into 5 subgroups sacrificed at 4 h, 12 h, 24 h, 48 h and 72 h after SC. The rat SC model was prepared by lithium-pilocarpine chemical method. The protein expression of GFAP and NF-κB was detected by the method of immunohistochemistry. The mRNA expression of GFAP was detected by RT-PCR. The neuronal apoptosis was observed by TdT-mediated dUTP nick end labeling (TUNEL). RESULTS: Compared with NS group, the GFAP positive cells was increased in SC group (P<0.05). Compared with SC group, the expression of GFAP was significantly reduced in BC group (P<0.05). Compared with NS group, the NF-κB positive cells was increased in SC group (P<0.05). Compared with SC group, the expression of NF-κB was significantly reduced in BC group. RT-PCR showed that the expression trend of GFAP mRNA was similar to that of the protein. Compared with NS group, the TUNEL positive cells in the hippocampus CA1 area in SC group increased significantly 12 h after SC (P<0.01), and reached a peak at 48 h. After the intervention with BC, the TUNEL positive cells decreased significantly between 12~48 h after SC (P<0.05 or P<0.01), but the number of TUNEL positive cells remained significantly greater than that in NS group (P<0.05). CONCLUSION: The expression of GFAP and NF-κB in the hippocampus increased after SC in rats. Baicalin decreases the expression of GFAP and NF-κB in hippocampus of rats with pilocarpine-induced seizures, and reduces the number of neuronal apoptosis, suggesting that baicalin may protect against the brain damage caused by status convulsion. 相似文献
102.
AIM: To explore the effects and mechanism of eleutheroside (ETS) B or E on the proliferation of HBZY-1 cells treated with high glucose. METHODS: The HBZY-1 cells were cultured under high glucose condition. The 4th generation of HBZY-1 cells was used for determining the optimal cell density, which was consistent with the growth regulation curve of the cells. The cells were divided into 6 groups: low glucose (LG) group, high glucose (HG) group, high glucose plus ETS-B/E (low dose, medium dose and high dose) groups, and high glucose plus losartan (LTG) group. After all cells were treated with the corresponding drugs at 24 h, 48 h and 72 h, the inhibitory rate of the proliferation was measured, and the expression of TGF-β1 and PPARγ was detected by immunocytochemistry and Western blotting. RESULTS: The best cell density was 2 000 cells/well, which was complied with the basic rules of the cell growth, and high glucose significantly promoted the HBZY-1 cell proliferation. At each time point, the inhibitory effects of ETS-B/E were significantly different between HG group and LTG group on the proliferation of the HBZY-1 cells (P<0.05). The expression of TGF-β1 was significantly inhibited, and the expression of PPARγ was significantly promoted by ETS-B/E (P<0.05). ETS-E showed stronger effect than ETS-B (P<0.05) in a concentration- and time-dependent manner. CONCLUSION: ETS-B/E significantly inhibits the proliferation of HBZY-1 cells under high glucose condition by decreasing TGF-β1 expression and promoting PPARγ expression. 相似文献
103.
甲壳素对连作平邑甜茶生长、光合及抗氧化酶的影响 总被引:2,自引:0,他引:2
以苹果连作土盆栽的平邑甜茶幼苗为试材,探讨了0、0.5、1.0和2.5 g·kg-1不同施入量的甲壳素对其光合速率、活性氧含量及抗氧化酶活性的影响。结果表明,1.0 g·kg-1的甲壳素处理能显著促进幼苗株高、地径,干样质量和根冠比的增加,根冠比为对照的1.51倍;明显提高了幼苗叶片光合色素含量、净光合速率和蒸腾速率,其中光合速率为对照的1.30倍;同时提高了幼苗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,分别为对照的1.10倍、1.85倍、1.77倍和1.43倍;减少了叶片丙二醛(MDA)、过氧化氢(H2O2)和超氧阴离子的积累,分别为对照的73%、62%和34%,降低了脯氨酸(Pro)和可溶性糖含量。当甲壳素施用量为2.5 g·kg-1时则显著抑制平邑甜茶幼苗生长,降低幼苗叶片光合速率和抗氧化酶活性,并使超氧阴离子和脯氨酸含量明显上升。因此,适宜用量的甲壳素能减轻苹果的连作障碍。 相似文献
104.
Use of Porcine Meal in Plant‐based Practical Diets for Pacific White Shrimp,Litopenaeus vannamei
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Two growth trials were conducted to evaluate and confirm the efficacy of a porcine meal (PM) with high protein content (>90%) as an alternative feed ingredient in commercial‐type feed formulation for Pacific white shrimp, Litopenaeus vannamei. Six experimental diets were formulated for the two growth trials. The first five diets contained increasing levels (0, 1, 2, 4, and 6%) of PM as a replacement for soybean meal in a plant‐based diet with low inclusion level (6%) of fish meal (FM). The last experimental diet was produced utilizing 4.2% PM to completely replace FM. In Trial 1, shrimp (1.5 g initial mean weight, 20 shrimp/tank, n = 4) were offered test diets for 6 wk in a semirecirculation system. At the end of Trial 1, shrimp fed with the diet containing 6% PM exhibited significantly enhanced weight gain (WG), feed conversion ratio (FCR), and survival compared to those fed with the diet devoid of FM. As survival was poor across all treatments and different densities could mask growth results the trial was repeated. In Trial 2, shrimp (0.85 g, 15 shrimp/tank, n = 4) were offered diets for 6 wk. Dietary supplementation of PM at 6% significantly improved WG, FCR, and apparent net protein retention in contrast with the treatment devoid of FM, confirming the same trends in Trial 1. No significant difference was detected in protein, lipid, moisture, and mineral profiles of whole‐body shrimp as well as survival across all the treatments. Results of this study indicate that PM is a good high protein source in shrimp feeds, which can be included up to 6% in the low FM‐based diet without compromising the growth of shrimp. 相似文献
105.
106.
以引进的美国核桃品种‘维纳’、‘艾米格’和新疆本地品种‘温185’和‘新新2号’为试材,研究分析不同品种的植物学和生物学特性、花芽分化过程以及相关成花基因的表达。结果显示:4个品种在树体生长、枝芽发育特性、物侯期以及成花率和坐果率方面没有明显差异。在结果枝同一坐果部位形成双果、三果或以上的能力‘新新2号’明显强于‘维纳’,但‘新新2号’的果实大小和单果重都低于‘维纳’,而‘维纳’平均结果母枝上形成侧花芽的数量和坐果率高于‘新新2号’。从萌芽到开花结束直至当年侧芽发育过程中‘维纳’混合芽中的JrFT和JrLFY基因表达也高于‘新新2号’,而同时期的JrFLC基因表达则相对较低,说明‘维纳’在花芽分化和花器官形成过程中通过高表达JrFT和JrLFY基因,低表达JrFLC基因促进结果母枝上形成大量侧花芽,并利用大量侧花芽抽生结果枝坐果,形成较大的果实,使得最终产量并不低于同一个坐果部位大量着生2个或多个果实的‘新新2号’品种。 相似文献
107.
108.
109.
Annual C input to soil is a major factor affecting soil organic carbon (SOC) dynamics. However different types of C-sources can have different behaviour, in relation to their chemical characteristics and how they interact with soil. Root-derived C, in particular, should be more efficient than other organic materials as a result of the physicochemical and biological characteristics of the surrounding environment, leading to a reduction in the C decomposition rate.To test this hypothesis, we considered a long-term experiment underway in Northern Italy since 1962, comparing permanent meadow and 6 different crop rotations over a wide range of nutrient inputs, in both organic and inorganic forms. C inputs from amendments were measured and those from crops were calculated using allometric functions and crop and residues yields. The time evolution of SOC was studied through a single-pool, first-order kinetic model, allowing the estimation of humification coefficients for residues, roots, farmyard manure and cattle slurries.The highest value of the humification coefficient was estimated for farmyard manure, which confirmed its high efficiency in stabilising SOC content. Root C presented a humification coefficient 1.9 times higher than above-ground plant materials while slurries were intermediate, with a humification coefficient roughly half that of farmyard manure and even lower that of roots.The quality of C input thus seems of fundamental importance for evaluating the sustainability of different cropping systems in terms of SOC dynamics. 相似文献
110.
基于改进GM(1,N)模型的我国大豆价格影响因素分析及预测研究 总被引:2,自引:0,他引:2
大豆是我国重要的粮食作物和油料作物,其价格对于国民经济尤其是农业经济的影响意义深远。大豆价格的稳定对于我国大豆市场的健康发展有着重要的现实意义。在灰色理论的基础上,提出了一种改进GM(1,N)大豆价格预测模型,首先运用灰色关联分析法对我国大豆价格的影响因素进行分析,选择主要的影响因素;再将这些影响因素作为模型的相关因素变量,构建GM(1,N)大豆价格预测模型。采用2010-2015年的大豆数据进行实证研究,模型选取国内大豆自给量、世界大豆产量、国民消费价格指数、消费者信心指数4个变量作为相关因素变量;模型预测误差为2.10%,预测精度较高,能够较好地掌握大豆价格的变化规律,可以为大豆价格市场预测及国家宏观政策的制定提供理论指导。 相似文献