鹅黑素皮质素受体-4基因的克隆与序列分析

黑素皮质素受体-4（Melanocortin receptor-4,MC4R）是其黑素皮质素受体MCR（MC1-5R）家族成员之一,属G蛋白偶联受体。它可与瘦蛋白、神经肽、α-黑素细胞刺激素等一起调节动物体重和采食量。参考鸡MC4R基因序列设计引物,克隆并测序了鹅MC4R基因。结果表明,鹅MC4R基因编码区全长996 bp,其核苷酸序列与鸡的同源性为95.3%,与人、牛、猪等哺乳动物同源性在75%-79%;其氨基酸序列与鸡的同源性达到98.5%。构建哺乳类、鸟类和鱼类MC4R基因核苷酸进化树显示,鹅较早地与哺乳类动物分化开来。分析MC4R蛋白的氨基酸残基特性参数表明,MC4R的7次跨膜结构与MC4R的亲水性区域、电荷密度以及氨基酸残基位于表面概率的变化规律相一致。     

Integrated disease management of ascochyta blight in pulse crops

Ascochyta blight causes significant yield loss in pulse crops worldwide. Integrated disease management is essential to take advantage of cultivars with partial resistance to this disease. The most effective practices, established by decades of research, use a combination of disease-free seed, destruction or avoidance of inoculum sources, manipulation of sowing dates, seed and foliar fungicides, and cultivars with improved resistance. An understanding of the pathosystems and the inter-relationship between host, pathogen and the environment is essential to be able to make correct decisions for disease control without compromising the agronomic or economic ideal. For individual pathosystems, some components of the integrated management principles may need to be given greater consideration than others. For instance, destruction of infested residue may be incompatible with no or minimum tillage practices, or rotation intervals may need to be extended in environments that slow the speed of residue decomposition. For ascochyta-susceptible chickpeas the use of disease-free seed, or seed treatments, is crucial as seed-borne infection is highly effective as primary inoculum and epidemics develop rapidly from foci in favourable conditions. Implemented fungicide strategies differ according to cultivar resistance and the control efficacy of fungicides, and the effectiveness of genetic resistance varies according to seasonal conditions. Studies are being undertaken to develop advanced decision support tools to assist growers in making more informed decisions regarding fungicide and agronomic practices for disease control.     

醋糟代替棉籽壳栽培平菇的研究

本文从试验中得出,以醋渣全部或部分取代棉籽壳栽培平菇的配料技术和适宜的平菇菌种。     

城镇净化沼气池残渣还田处理试点研究

通过对城镇生活污水净化沼气池残渣与农村沼气池沼渣性质的分析比较及农田土壤成分的分析，提出了城镇净化沼气池残渣还田处理研究。在连续三年对小麦，油菜施用城镇净化沼气池残渣还田处理效果研究的基础上，总结出了实用的施用技术。     

木材加工企业热能源自给问题的探讨

木材工业中干燥耗能与能源紧的矛盾日益突出，国外木材加企业在热能源自给方面技术已趋成熟，实施效果良好，为了增强国内企业节约能源的意识，本文详细测算分析了木材加工企业废料数量关系，提出了变废料为燃料、实现企业热能源自给的设想和依据。     

粮谷中丙线磷残留分析方法

对粮谷中丙线磷残留量的检测，采用新法以甲醇提取样品，经液液萃取净化后，再以气相色谱／氮磷检测器测定，测定低限为０．００５μg/ml的浓度范围内，标准曲线呈良好的线性关系γ＝０．９９６，变异系数为５．８％－７．８％，回收率为７２．０％－１００．２％。取代了常规法的繁锁操作程序，使操作步骤更加简便，分析速度快且灵敏度高，适用范围广。     

直接竞争ELISA检测氯霉素残留的方法建立及初步应用

用混合酸酐法合成氯霉素的CAP抗原,用过碘酸钠氧化法合成CAP酶标记抗原,用ELISA方法对合成的CAP抗原、CAP酶标记抗原进行鉴定表明其与抗CAP单克隆抗体具有特异性反应。在此基础上建立了包被二抗的直接竞争ELISA方法。特异性试验结果表明,CAP琥珀酸酯、CAP琥珀酸钠盐的交叉反应率分别为107%、106.4%;甲砜霉素、氟甲砜霉素与CAP的交叉反应率小于0.016%;与其他结构类似物间未见交叉反应。该方法对CAP的检测限可达到0.1μg/L,IC50为11.6μg/L,线性范围0.1～100μg/L,批内变异系数小于11.5%,批间变异系数小于19.6%。对猪肉、鸡肉、蜂蜜分别添加1.0、3.0、10.0、30.0μg/L CAP标准品,回收率61.0%～102.0%,变异系数为6.6%～15.8%;对猪肉、鸡肉、蜂蜜中CAP的最低检测限分别为0.48、0.42、1.20μg/kg。     

Effect of Tillage and Plant Residue Management Practices on Shrinkage of a Vertisol

A study was carried out to determine the effects of different tillage and plant residue management practices on different soil moisture and shrink-swell properties of a Vertisol (very fine, semctitic, thermic, chromic Haploxerert (with less than 1 % slope). The core samples were collected in July (after harvesting of lentil) and in November (after planting of wheat) 1993. The two tillage methods were moldboard (T1) and chisel (T2). The two plant residue incorporation dates were in August (R1) and in October (R2). Results indicated that T2 tends to give higher water holding capacity and available moisture than T1. Immediate incorporation of plant residue (R1) tends to increase the bulk density and to decrease soil specific volume and void ratio when compared to late incorporation treatment (R2). In general, the two tillage treatments tend to decrease moisture availability, shrinkage characteristics, soil specific volume, and void ratio. They also tend to increase the soil shrinkage (subsidence and cracks volume) and bulk density. Chisel plough is recommended in Vertisols, if it is to be used before the rainfall.     

Effect of Tillage System on Soil Strength and Bulk Density of Vertisols

Improving the soil environment for seedling emergence and plant growth is possible using a good tillage system. From March 1993 until June 1994, the effect of tillage system and plant residue incorporation practices on the bulk density and the soil strength of the surface layer of a vertisol (very fine, smectitic, thermic, chromic Haploxerert), on a less than 1 % slope, was investigated. The two tillage methods were moldboard (T1) and chisel (T2). The two plant residue incorporation dates were in August (R1) and in October (R2). Results indicated that R1 gave significantly higher bulk density than R2, while the different tillage treatments had no significant effect. Soil strength was significantly lower under T1 and R2 than under the other treatments.     

牲畜排泄物综合利用路径探究——以盐城奶牛场牲畜排泄物为例

随着中国畜禽养殖规模扩大,集约化程度不断提高,同时伴随化肥工业的迅速发展,使有机粪肥大量闲置,不能及时还田,导致环境污染。本文旨在了解沼气利用现状的基础上,研究牲畜排泄物的综合利用方式,探索畜禽粪便资源化利用的技术途径,为实现养殖场的可持续发展和发展生态农业提供参考。