鹅黑素皮质素受体-4基因的克隆与序列分析

黑素皮质素受体-4（Melanocortin receptor-4,MC4R）是其黑素皮质素受体MCR（MC1-5R）家族成员之一,属G蛋白偶联受体。它可与瘦蛋白、神经肽、α-黑素细胞刺激素等一起调节动物体重和采食量。参考鸡MC4R基因序列设计引物,克隆并测序了鹅MC4R基因。结果表明,鹅MC4R基因编码区全长996 bp,其核苷酸序列与鸡的同源性为95.3%,与人、牛、猪等哺乳动物同源性在75%-79%;其氨基酸序列与鸡的同源性达到98.5%。构建哺乳类、鸟类和鱼类MC4R基因核苷酸进化树显示,鹅较早地与哺乳类动物分化开来。分析MC4R蛋白的氨基酸残基特性参数表明,MC4R的7次跨膜结构与MC4R的亲水性区域、电荷密度以及氨基酸残基位于表面概率的变化规律相一致。

Cloning and Sequence Analysis of Melanocortin Receptor-4 Gene in Goose

Melanocortin receptor-4(MC4R) is one of members of the family of melanocortin receptors(MC1-5R) and belongs to the family of seven transmembrane G protein coupled receptors.It can cooperate with leptin,neuropeptide Y and melanocyte-stimulating hormone to regulate body weight and feeding.A pair of primers which were designed according to the sequence of chicken MC4R gene were used to amplify the goose MC4R gene.The results showed that sequence of goose MC4R gene was 996 bp,and homologous comparison showed 95.3% and 98.5% homology at the nucleotide and amino acid levels with chicken respectively,and 75% to 79% with mammal(human,cattle and pig,etc) in nucleotide sequence.The hylogenetic tree among mammalian,birds and fish showed goose earlier differentiated from mammals.The hydrophilicity,averagy charge and surface probability of amino acid residues varied with MC4R seven transmembrane process.