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81.
不同感染量REV对鸡免疫反应和细胞毒性作用的影响 总被引:4,自引:0,他引:4
用不同剂量网状内皮增生症病毒(REV)感染肉鸡和SPF鸡后,检测血液中T淋巴细胞对ConA的反应和NK细胞、细胞毒T细胞(CTL)的细胞毒性作用以及NDV抗体生成变化等,观察REV感染对机体非特异性、特异性细胞免疫反应和体液免疫反应的影响。结果表明无论高剂量还是低剂量REV感染均造成体液免疫和非特异性细胞免疫抑制,而且高剂量比低剂量对免疫功能的抑制作用更强,但对NK细胞和细胞毒T细胞的细胞杀伤活性却有升高趋势,在抗肿瘤方面发挥一定的作用。这种结果说明REV感染对机体的免疫抑制是有选择性的,且抑制程度与感染病毒量有关。 相似文献
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85.
用PCR扩增了MBP—ZOT质粒上的ZOT基因,该基因编码264~399位氨基酸残基C末端的15ku △G片段,并与经改造后的质粒P^BCX、连接。将重组后的质粒PMLAG在大肠杆菌BL21中表达,将表达的目的蛋白纯化,再将p^BCX-△G片段与传染性腔上囊炎病毒VP2包涵体蛋白通过滴鼻免疫途径对14日龄SPF鸡进行联合免疫试验,分别于免疫后第10、20d采集血清样品,进行ELISA试验,检测免疫后IBD抗体效价。动物免疫试验发现:融合蛋白P^BCX △G起到了明显免疫增强效果,与VP2重组蛋白联合免疫2次后,血清IBD的VP2 IgG抗体效价是单独用VP2免疫组的3倍,明显高于空白免疫组。 相似文献
86.
ALONSO N. GUTIÉRREZ MICHAEL DEVEAU LISA J. FORREST WOLFGANG A. TOMÉ THOMAS R. MACKIE 《Veterinary radiology & ultrasound》2007,48(6):594-602
Feasibility of delivering a simultaneously integrated boost to canine nasal tumors using helical tomotherapy to improve tumor control probability (TCP) via an increase in total biological equivalent uniform dose (EUD) was evaluated. Eight dogs with varying size nasal tumors (5.8-110.9 cc) were replanned to 42 Gy to the nasal cavity and integrated dose boosts to gross disease of 45.2, 48.3, and 51.3 Gy in 10 fractions. EUD values were calculated for tumors and mean normalized total doses (NTD(mean)) for organs at risk (OAR). Normal Tissue Complication Probability (NTCP) values were obtained for OARs, and estimated TCP values were computed using a logistic dose-response model and based on deliverable EUD boost doses. Significant increases in estimated TCP to 54%, 74%, and 86% can be achieved with 10%, 23%, and 37% mean relative EUD boosts to the gross disease, respectively. NTCP values for blindness of either eye and for brain necrosis were < 0.01% for all boosts. Values for cataract development were 31%, 42%, and 46% for studied boost schemas, respectively. Average NTD(mean) to eyes and brain for mean EUD boosts were 10.2, 11.3, and 12.1 Gy3, and 7.5, 7.2, and 7.9 Gy2, respectively. Using helical tomotherapy, simultaneously integrated dose boosts can be delivered to increase the estimated TCP at 1-year without significantly increasing the NTD(mean) to eyes and brain. Delivery of these treatments in a prospective trial may allow quantification of a dose-response relationship in canine nasal tumors. 相似文献
87.
构建了泛素(ubiquitin,Ub)基因与密码子优化的PRRSVGP5蛋白(optiGP5)基因融合表达质粒pIR—Ub—optiGP5。间接免疫荧光和Western—blot分析表明,重组质粒转染293T细胞后能够瞬时表达。将pIR—optiGP5和pIR—Ub—optiGP5两种质粒DNA肌肉注射免疫BALB/c小鼠后,分别检测小鼠血清中抗GP5抗体、T淋巴细胞的增殖能力以及CTL活性。结果显示,pIR—optiGP5质粒免疫组在二免后可以检测到荧光抗体,而pIR—Ub—optiGP5质粒免疫组一直未检测到抗GP5抗体,但该免疫组的T淋巴细胞增殖指数及针对GP5的特异性CTL反应数值明显高于pIR—optiGP5质粒免疫组。这表明泛素与GP5的融合表达可增强细胞免疫反应。 相似文献
88.
H. Khatib I. Zaitoun Y.M. Chang C. Maltecca & P. Boettcher 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2007,124(1):26-28
In dairy cattle, many studies have reported quantitative trait loci (QTL) on the centromeric end of chromosome 14 that affect milk production traits. One of the candidate genes in this QTL region – thyroglobulin (TG) – was previously found to be significantly associated with marbling in beef cattle. Thus, based on QTL studies in dairy cattle and because of possible effects of this gene on fat metabolism, we investigated the association of TG with milk yield and composition in Holstein dairy cattle. A total of 1279 bulls from the Cooperative Dairy DNA Repository Holstein population were genotyped for a single nucleotide polymorphism in TG used previously in beef cattle studies. Analysis of 29 sire families showed no significant association between TG variants and milk production traits. Within‐sire family analysis suggests that TG is neither the responsible gene nor a genetic marker in association with milk production traits. 相似文献
89.
The effects of field aging (0–28 days) and pheromone loading rate on the longevity of red rubber septa loaded with the sex
pheromone blend of the oriental fruit mothGrapholita molesta (Busck), were evaluated in North Carolina apple orchards in 2002. Separate field tests examined the influence of trap height
and pheromone loading rate of rubber septa on trap catches of adultG. molesta males in an abandoned orchard. The loss of the major pheromone component, (Z)-8-dodecenyl acetate (Z8–12:OAc), from red rubber
septa over a 4-week period exhibited a relatively constant release rate with 30, 100 and 300 μg pheromone. Trap catch was
significantly higher in pheromone traps placed in the upper canopy than in those in the lower canopy. Pheromone traps baited
with 100μg lures caught more moths compared with those loaded with 300 μg. There was no apparent relationship between pheromone
trap catch and septa age, with trap catch appearing to be primarily a function ofG. molesta population density.
http://www.phytoparasitica.org posting May 14, 2006. 相似文献
90.
Sung Jae Shin Seung Won Shin Mi Lan Kang Deog Yong Lee Moon-Sik Yang Yong-Suk Jang Han Sang Yoo 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(4):383-392
We previously induced protective immune response by oral immunization with yeast expressing the ApxIIA antigen. The ApxI antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection; therefore, the protective immunity in mice following oral immunization with Saccharomyces cerevisiae expressing either ApxIA (group C) or ApxIIA (group D) alone or both (group E) was compared with that in two control groups (group A and B). The immunogenicity of the rApxIA antigen derived from the yeast was confirmed by a high survival rate and an ApxIA-specific IgG antibody response (p < 0.01). The highest systemic (IgG) and local (IgA) humoral immune responses to ApxIA and ApxIIA were detected in group E after the third immunization (p < 0.05). The levels of IL-1β and IL-6 after challenge with an A. pleuropneumoniae field isolate did not change significantly in the vaccinated groups. The level of TNF-α increased in a time-dependent manner in group E but was not significantly different after the challenge. After the challenge, the mice in group E had a significantly lower infectious burden and a higher level of protection than the mice in the other groups (p < 0.05). The survival rate in each group was closely correlated to the immune response and histopathological observations in the lung following the challenge. These results suggested that immunity to the ApxIA antigen is required for optimal protection. 相似文献