秸秆生物炭对黄土区农田土壤养分和作物生长的影响

旨在探索生物炭对黄土区农田的具体应用效果。采用大田试验，在不施氮肥和施氮肥(187.5 kg/hm2)处理下，分别设计了不同生物炭用量(0、6、12、24、48 t/hm2)试验，来观察对黄土高原农田土壤养分和玉米生长的影响。结果发现：当施入187.5 kg/hm2氮肥时，24~48 t/hm2范围内的生物炭施用量对土壤养分促进作用效果最佳，其中48 t/hm2时全氮超出对照26.6%，碱解氮可超过对照约27.67%；6~12 t/hm2的范围时，玉米茎粗、叶绿素在生长中期均相对处于优势，且玉米养分含量和产量结果优于生物炭量大的处理。不施氮的情况下，生物炭也可促进土壤和玉米籽粒养分的积累。其中6~12 t/hm2时，土壤和玉米籽粒养分含量相对较高；24~48 t/hm2时玉米产量结果最佳，比对照高1468.2 kg/hm2，约13.39%。结果表明，生物炭的添加对土壤的养分影响较大，且一定程度上依赖于氮肥的投入情况。     

两种不同镁盐在牛粪堆肥高温期的保氮效果研究

利用恒温培养箱模拟堆肥高温期,添加硫酸镁、磷酸二氢钾和含镁废渣,研究磷酸铵镁(MAP)反应中各成分的保氮效果以及含镁废渣作为堆肥过程中氮素固定剂的可行性。结果表明:添加镁盐固定剂显著降低了堆体中的pH值,减少了氮素损失。同时添加硫酸镁和磷酸二氢钾的保氮效果最佳,添加相同比例的含镁废渣和硫酸镁的保氮效果无显著差别。含镁废渣添加量的保氮效果与其添加量成正相关,添加质量比为2%、4%、8%的含镁废渣的保氮率分别为6.15%、10.13%、19.51%。添加镁盐会提高堆体中的EC值,降低GI值,各处理均可达到腐熟。说明可以利用镁盐作为堆肥保氮固定剂,而含镁废渣因其价格低廉,更具应用前景。     

几种利用乳中尿素氮浓度估测尿氮排泄量模型的评价

本文利用34组国内外报道的荷斯坦牛乳中尿素氮浓度和尿氮排泄量的实测数据，对目前提出的部分利用MUN估测尿氮排泄量的模型进行了比较。结果表明，Zhai（2005）提出的模型[UN（g/d）：10．1×MUN（mg／dL）＋47.3]预测效果较好（P〉0．05）。划分MUN浓度范围分别建立模型可能会提高预测的准确度。此外，根据34组数据做简单回归：UN（g/d）=12．78×MUN（mg/d1）＋28．15（n=34，R2=0．59）。     

三峡库区不同植被覆盖对土壤氮的影响

不同植被类型影响着土壤养分的积累、分布与循环,而土壤氮素是植被生长的重要限制性元素。通过分析宜昌点军区3种植被类型(柏树地、橘树地、菜地)覆盖下土壤氮素的变化情况,研究了不同植被对土壤氮素各形态的影响。结果表明,土壤全氮、硝态氮和微生物氮都是柏树地显著大于菜地和橘树地,而菜地和橘树地之间无显著性的差异;土壤矿化氮和微生物氮/全氮的变化顺序是柏树地橘树地菜地。说明不同植被覆盖对土壤氮有显著的影响,柏树地更有利于土壤氮的积累,氮的有效性也最高,由此认为柏树长期生长有益于土壤氮的改善。     

1例藏獒三氮脒中毒的生化检验及治疗

用三氮脒以3.5 mg/kg的剂量对患有附红细胞体病的藏獒进行肌肉注射,隔日用同等剂量的三氮脒肌肉注射,24 h后,藏獒发生以神经症状为主的中毒现象,并表现出食欲废绝,后肢发软,四肢抽搐呈划水状,卧地不起,昏迷,体温正常临床症状。经血清生化检验,藏獒的尿素氮、肌酸激酶等的生化指标明显升高,血糖降低占病例的100%。经过5 d的药物治疗后,中毒藏獒基本恢复健康。     

Effects of soybean variety and Bradyrhizobium strains on yield,protein content and biological nitrogen fixation under cool growing conditions in Germany

Soybean (Glycine max (L.) Merr.) is able to fix atmospheric nitrogen in symbiosis with the bacteria Bradyrhizobium japonicum. Because these bacteria are not native in European soils, soybean seeds must be inoculated with Bradyrhizobium strains before sowing to fix nitrogen and meet their yield potential. In Central Europe soybean cultivation is still quite new and breeding of early maturing soybean varieties adapted to cool growing conditions has just started.Under these low temperature conditions in Central Europe the inoculation with different, commercially available Bradyrhizobium inoculants has resulted in unsatisfactory nodulation. The aim of this study was: (i) to test the ability of commercially available inoculants to maximize soybean grain yield, protein content and protein yield, (ii) to study the interaction of different inoculants with different soybean varieties for two different sites in Germany under cool growing conditions over three years and (iii) to determine the variability of biological nitrogen fixation. Field trials were set up on an organically managed site at the Hessische Staatsdomäne Frankenhausen (DFH) and on a conventionally managed site in Quedlinburg (QLB) for three consecutive seasons from 2011 to 2013. Three early maturing soybean varieties—Merlin, Bohemians, Protina—were tested in combination with four different Bradyrhizobium inoculants—Radicin No.7, NPPL-Hi Stick, Force 48, Biodoz Rhizofilm—and compared with a non-inoculated control. Effective inoculation with Bradyrhizobium strains increased grain yield, protein content and protein yield by up to 57%, 26% and 99%, respectively. Grain yield, protein content and protein yield were generally higher in DFH. Average grain yield was 1634 kg ha⁻¹ in QLB (2012–2013) and 2455 kg ha⁻¹ in DFH (2011–2013), average protein content was 386 g kg⁻¹ in QLB and 389 g kg⁻¹ in DFH and average protein yield was 650 kg ha⁻¹ in QLB and 965 kg ha⁻¹ in DFH. The percentage of nitrogen derived from air (Ndfa) ranged between 40% and 57%. Soybeans inoculated with Radicin No. 7 failed to form nodules, and crop performance was identical to the non-inoculated control. Biodoz Rhizofilm, NPPL Hi-Stick and Force 48 are suitable for soybean cultivation under cool growing conditions in Germany. Interactions between soybean variety and inoculant were significant for protein content and protein yield at both sites, but not for nodulation, grain yield, thousand kernel weight and Ndfa. The variety Protina in combination with the inoculant Biodoz Rhizofilm can be recommended for tofu for both tested sites, while Merlin and Protina in combination with Biodoz Rhizofilm are recommended for animal fodder production in DFH. Animal fodder production was not profitable in QLB due to low protein yields.     

On-farm evaluation of an active optical sensor performance for variable nitrogen application in winter wheat

Winter wheat (Triticum aestivum L.) represents almost 50% of total cereal production in the European Union, accounting for approximately 25% of total mineral nitrogen (N) fertilizer applied to all crops. Currently, several active optical sensor (AOS) based systems for optimizing variable N fertilization are commercially available for a variety of crops, including wheat. To ensure successful adoption of these systems, definitive measurable benefits must be demonstrated. Nitrogen management strategies developed based on small-scale plot research are not always meaningful for large-scale farm conditions. In 2010–2012 (5 site-years) on-farm study was implemented in northern Poland utilizing a strip-trial design. The objective was to evaluate the performance of AOS in combination with a built-in algorithm for variable N rate fertilization. In this study, the reference uniform N rates (farmer’s practice) were comparable to optimum variable N rate recommendations. Side-by-side comparisons of uniform and variable N application revealed inconsistent benefits in terms of grain yield, grain protein content (GPC), N use and N use efficiency (NUE). Anticipated yield increases and/or reduced N rates are typical drivers for AOS adoption. Significant yield increases are not easily attained on farms with winter wheat yields already close to maximum yield potential. Thus, sensor-based variable N rate recommendations for fields previously fertilized with relatively low uniform N rates would often entail more appropriate allocation (redistribution) of the same amount of total N. This would minimize N surplus in areas of lower productivity and to improve the sustainability of N management overall.     

Fertilizer value of nitrogen in hen and broiler manure after application to spring barley using different application timing

The nitrogen (N) fertilizer effect of layer hen and broiler manure applied at different times on spring barley yield was studied in seven Swedish field experiments during 2005–2008. Two experiments had parallel field incubations to study N release after fertilizer application. The effect of total N in manure on N offtake was 30–40% that of mineral N, except in a dry year, when the effect was very low. Although the relative proportions of ammonium N, uric acid N and other N differed between the hen and broiler manure, the effect of total N was similar for both. In field incubations, mineral N decreased from 75 to 60% of total N applied in hen manure, whereas it increased from 20 to 50% in broiler manure, because of net immobilization and release, respectively. The limited fertilizer nitrogen replacement value, corresponding to only 30–40% of total N, could be as a result of ammonia volatilization after rather shallow incorporation with harrow. Net N release from broiler manure lasted for 6–8 weeks after application, after which it generally ceased. In some cases, manure application in early spring gave better yield effects than application at sowing, probably because of better synchronization of the N release with crop N requirements. The residual N effect on the N offtake in crop in the year after manure application was on average 3% of the total N applied, equivalent to a fertilizer replacement value of about 6%.     

Effect of wilting,silage additive,PEG treatment and tannin content on the distribution of N between different fractions after ensiling of three different sainfoin (Onobrychis viciifolia) varieties

Sainfoin (Onobrychis viciifolia) is a tanniniferous, leguminous plant that has potentially beneficial effects on protein utilization in ruminants. As ensiling causes protein breakdown and elevated levels of buffer soluble N (BSN), we studied the distribution of N before and after ensiling sainfoin. Three varieties of sainfoin were either direct‐cut and frozen directly or wilted and frozen before later ensiling in mini‐silos with and without acidification with Promyr (PM; an acidifying commercial mixture of propionic and formic acid) and with or without polyethylene glycol (PEG). Extractable tannins (ET) and protein‐bound tannins (PBT) were measured with an HCl/butanol method in an attempt to correlate tannin levels to N fractions. The sainfoin silages showed good ensiling characteristics and had relatively high concentrations of undegraded protein. The effect of wilting on BSN levels (g/kg N) was dependent on sainfoin variety (P < 0·001). PEG increased and PM decreased the level of BSN in the silages (P < 0·001). PM treatment also produced less non‐protein N and ammonia‐N (P < 0·05) as compared with no additive. Addition of PEG to the silage increased the BSN‐proportion 1·8‐ and 2·6‐fold for both DM stages. A strong tannin‐protein binding effect is, therefore, confirmed in sainfoin. However, correlations between tannin levels (ET and PBT) and BSN were poor in the (non‐PEG) silages, indicating either that the HCl/butanol method is unsuitable for measuring tannin in silages or that qualitative attributes of tannins are more relevant than quantitative. The HCl/butanol method seems therefore not to be useful to predict degradation of protein in sainfoin silages.     

白洋淀湖心岛分散式鸭养殖污染控制技术研究

白洋淀分散鸭养殖产生的污染物是导致其水体污染的主要来源之一。依据“减量化、就地处置资源化、养分循环”思路,提出湖心岛分散鸭养殖污染控制模式,主要包括：鸭粪收集系统（收集网、径流和冲洗水收集沟）、鸭粪沼气发酵与利用系统、沼液湿地净化和沼渣利用系统。选择安新县安新镇王家寨村占地5 000 m2养殖1 500只鸭的湖心岛建立综合控制示范工程。2010年4~11月,示范工程在典型降雨日和非降雨日排水的COD、总氮、总磷分别为351 mg/L、74.2 mg/L、7.1 mg/L和214 mg/L、42.1 mg/L、5.3 mg/L,水质符合《畜禽养殖业污染物排放标准》（GB 18596-2001）；这是由于源头收集系统收集了陆上活动区40%的鸭粪,同时末端处理系统（沉淀池+人工湿地）对养鸭废水污染物的高效削减。沼气池产生的沼气和剩余的沼液沼渣可以作为能源和肥料加以利用。本文集成湖心岛分散鸭养殖污染控制模式简便、适用,具有推广价值。