刺槐叶粉喂兔的饲养价值研究

对四川省１９８８～１９９１年生产的商品刺槐叶粉（２０个）样本的概略养分进行分析，并用磷钼酸—钨酸钠比色法测定部分样本的单宁含量及用色谱法测定β—胡萝卜素含量。同时，用全收粪尿法作了新西兰白兔对刺槐叶粉养分利用的代谢试验。结果表明，２０个样本刺槐叶粉成分含量均值为：干物质（ＤＭ）８８．１７％，总能（ＧＥ）１９．０６ＭＪ／ｋｇ，粗蛋白（ＣＰ）１８．８６％，粗脂肪（ＥＥ）４．１２％，粗纤维（ＣＦ）１８．２６％，粗灰分（ａｓｈ）９．９％，钙（Ｃａ）２．２１％，磷（Ｐ）０．１５％，β—胡萝卜素含量丰富（９０．９８～２４２．６８ｍｇ／ｋｇ），单宁（ｔａｎｎｉｎ）含量较高（１．６４％～２．８７％）（ＤＭ基础）。刺槐叶粉养分表观消化率：ＤＭ３１．５６％，ＧＥ２２．５４％，ＣＰ１６．５９％，ＥＥ６７．３２％，ＣＦ—１２．４６％。本研究表明：刺槐叶粉用作兔日粮组分的饲养价值较低     

泾川县刺槐薪炭林生长状况分析

通过对泾川县刺槐薪炭林的调查研究 ,分析其生长变化规律 ,并建立林分生长和采薪收获数学预测模型 ,为生产经营提供一定的科学依据     

饲料型刺槐微体快繁技术研究

以从韩国引进的四倍体饲料型刺槐为材料,研究了添加在培养基中不同激素组成、浓度、培养条件等因素对其增殖快繁、生根、移栽的影响。结果表明：MS+6-BA0.5mg/L+NAA0.05mg/L、蔗糖30g/L,培养温度25--27℃,光强1500LX左右,每天光照12h,对嫩梢增殖最有利。1/2MS+NAA0.5mg/L,蔗糖20g/L,温度25-27℃,光强2500LX,光照12h,培养20d,生根率可达81%。     

Development of Polymorphic Microsatellite Markers of Obolodiplosis robiniae (Haldeman) (Diptera: Cecidomyiidae), a North American Pest Invading Asia

Microsatellite markers were developed for epidemiological studies on the black locust gall midge Obolodiplosis robiniae (Haldeman) (Diptera: Cecidomyiidae), a native North America pest introduced to Europe and Asia. Polymorphism at each locus was tested on 68 individuals from six populations reared from infected host leaves of Robinia pseudoacacia L. collected in China. Fourteen loci were found to be polymorphic, with the number of alleles ranging from 3 to 10. The observed heterozygosity varied evenly from 0.2667 to 0.6540. For populations, the observed heterozygosity ranged from 0.1429 to 1.000. The allele frequency of the predominant allele varied from 0.250 to 0.500. All loci with negative F_ST values indicated heterozygote excess in each locus with six populations. Of 14 loci, four were observed to have F_ST values up to 0.05, which indicated negligible genetic differentiation within the population. Significant deviations (P < 0.05) from the expected Hardy–Weinberg equilibrium, as evaluated using the Markov chain algorithm for each locus and for all six populations, were observed, and genotypic linkage disequilibrium was clearly detected. These markers represent a useful tool to design strategies for integrated pest management and in the study of population evolution in this important introduced pest.     

五种杀虫剂对大庆草原蝗虫的田间防治效果

为筛选出防效较好的杀虫剂用于大庆地区蝗虫的防治,采用笼罩式方法,应用印楝素乳油、阿维菌素、丁烯氟虫腈乳油、氰戊菊酯和阿维·苏云菌共5种杀虫剂对大庆地区草原蝗虫进行了田间药效对比试验。结果表明:药后3d,阿维·苏云菌和印楝素防效为56.0%和60.0%,其余防治效果均高于76.0%。药后12d,阿维·苏云菌防效达到82.9%,其余4种杀虫剂均达到100.0%。说明化学杀虫剂作用效果迅速,生物杀虫剂作用效果缓慢,应根据蝗虫的发生情况选择适宜的药剂进行防治。     

The Special Expression and Comparison of the c-kit Protein in Spermatogenesis of Three Species of Locusts of Arcypteridae

The aim of this study was to elucidate the expression and regulation of the c.kit protein in spermatogenesis of locusts. Immunohistochemistry and biological statistics were used to investigate the expression of the c-kit protein in four representative phases of spermatogenesis of three dominant species of locusts of Arcypteridae （Orthoptera： Acridoidea）, namely, Omocestus viridulus （Linnaeus）, Euchorthippus unicolor （Ikonn.）, and Euchorthippus vittatus Zheng, and so on, in Siping area of Jilin Province, China. The results revealed the following： （1） There was weak positive expression of the c-kit protein in spermatogonia and the positive granules were thinner; （2） there was a strong positive expression of the c-kit protein in primary spermatocyte and the positive granules became the largest than in all developmental stages; （3） the c-kit protein positive expression became stronger in secondary spermatocyte, while the positive granules became thinner; （4） there was strong positive expression of the c-kit protein and the positive granules were thinner in mature sperm, which were distributed on its head and tail; （5） there were strong positive protein granules massing at the end of spermary; （6） the positive intensity of the c-kit protein in spermatogenesis was significantly different among different species of locusts. The data suggested that the c-kit protein may play a crucial role in spermatogenesis as well as maintain the physiological action of sperms and fertilization, regulate the developmental speed of spermatogenesis, and/or maintain species isolation, etc.     

杨树刺槐混交林及纯林土壤酶活性的季节性动态研究

测定了 2 2年生杨树、刺槐混交林及纯林根际和非根际土壤中与氮、磷养分转化相关的酶活性的季节性动态变化 .结果表明 ,混交后非根际土壤的脲酶、蛋白酶、磷酸酶活性比纯林提高 5 0 %～ 10 0 % ;但对于根际土壤 ,混交后杨树有所提高 ,而刺槐则有所下降 .说明混交后更有利于氮、磷养分的转化 ,并且杨树得益大于刺槐     

盐胁迫下ABA对刺槐幼苗体内腐胺,脯氨酸和保护酶系统的影响

盐胁迫下刺槐幼苗的生长明显受抑,相对含水量(RWC)降低,质膜透性变大,超氧物歧化酶(SOD)和过氧化氢酶(CAT)活性变小,同时大量积累游离脯氨酸和腐胺含量。外源ABA能缓和盐胁迫对生长的抑制,减少质膜透性,提高RWC,SOD和CAT活性,脯氨酸量被继续增加,但腐胺量却被减少,表明腐胺量的变化与幼苗的水分状况和遭受胁迫的程度一致,腐胺积累的多少可作为植物遭受胁迫程度的生理指标。     

四倍体刺槐离体培养及其不定根发育和叶片解剖观察

 .通过茎段离体培养建立了四倍体刺槐无性系的微体繁殖体系。结果表明:基本培养基为MS或WPM培养基。BA、NAA影响芽的增殖生长,在一定的范围内,BA对芽的增殖影响比NAA大,而NAA对芽的高生长影响比BA大,二者的比例对芽的增殖生长也有影响,芽增殖生长的最适BA和NAA组合应为BA0.5mg·L-1+NAA0.1mg·L-1。四倍体刺槐无性系生根的最适生长调节物质配比为NAA0.25mg·L-1+IBA0.4mg·L-1。对试管苗不定根发育过程及分步炼苗叶片结构的变化进行了解剖观察,发现试管苗嫩梢无潜伏根原基,不定根由诱生根原基发育形成,诱生根原基源于髓射线细胞的分裂和分化,植株叶解剖观察,进一步证明了分步炼苗可提高移栽成活率。     

基于特征点提取匹配的蝗虫切片图像的拼接和修复方法

由于环境和切片本身特性的影响,试验中获取的蝗虫切片总是不完整或者带有褶皱的。针对这一问题,提出了一种基于图像匹配的蝗虫切片拼接和修复方法,以序列切片中缺损切片的邻近切片图作为参考对象,对缺损切片图像进行拼接和修复。首先对切片进行小波降噪,降低噪声对匹配的影响;然后用尺度不变特征变换(scale.invariant feature transform,SIFT)算法和快速鲁棒特征(speed-up robust features,SIJRF)算法获取切片特征点并生成特征向量,完成切片的初始特征点匹配;随后利用随机采样一致(random sample consensus,RANSAC)算法进行匹配矫正,剔除匹配点对中的误匹配,并利用最小二乘法求解出单应性矩阵;最后用正确的匹配点对和单应性矩阵完成蝗虫切片的拼接,利用求得的空间映射模型找到褶皱部分相应的匹配域,完成对缺损部分的修复。试验表明:提出的切片拼接和修复方法的所提取的特征点的正确匹配率能够达到72.2%,并且运行速度以及匹配效果都能在一定程度上满足切片修复的要求,为后面进行蝗虫切片褶皱打开以及蝗虫体的三维重建提供了技术参考。