The effect of Astragaloside IV on immune function of regulatory T cell mediated by high mobility group box 1 protein in vitro

High mobility group box 1 protein (HMGB1), a potent pro-inflammatory cytokine, contributes to the pathogenesis of diverse inflammatory and infectious disorders. Some studies have illustrated the potential effect of HMGB1 on regulatory T cells (Tregs). Astragaloside IV (AST IV) isolated from a Chinese herb, Astragalus mongholicus, is known to have a variety of immunomodulatory activities. However, it is not yet clear whether AST IV possesses potential regulatory effect on the pro-inflammatory ability of HMGB1 with subsequent activation of Tregs. This study was carried out to investigate the antagonistic effects of different doses of AST IV on the immune function of Tregs mediated by HMGB1 in vitro. Tregs isolated from the spleens of mice were co-cultured with HMGB1 and/or AST IV. Cell phenotypes of Tregs were analyzed, and the contents of various cytokines in the cell supernatants as a result of co-culture and the proliferation of CD4⁺CD25⁻ T cells were determined. Results showed that HMGB1 stimulation resulted in significantly down-regulation of expressions of Tregs cell phenotypes. However, AST IV can rival the suppressing effect of HMGB1 on immune function of Tregs with a dose-dependent in vitro. These results indicate that AST IV has the potential therapeutic action on inflammation augmented by HMGB1.     

Sensitivity Analysis of Cantilever Box Beam Temperature Effects Based on the Prestressed Concrete Parameter Changes

On the construction of large-span prestressed box beam Cantilever, the parameter frequent changes in concrete modulus E, coefficient of thermal expansion α.In a certain temperature case, the change of concrete parameters will cause the changes on internal stress and strain of the bridge and the difference of the changes will affect the bridge safety. Accordingly,based on a bridge cross-section of a temperature field and combined with the actual bridge construction, give concrete Cantilever bridge parameters change, calculated and analyzed the sphere of influence of parameters on the stress and strain fields of bridge, asked the law of sensitivity on stress changes. Summarize the sensitivity factors of temperature stress which is used to improve bridge safety.     

小麦WPBF与高分子量谷蛋白基因上游Prolamin-Like box的特异结合

【目的】克隆普通小麦胚乳特异表达转录因子基因WPBF,对其原核表达条件进行优化,并验证其编码蛋白和小麦高分子量谷蛋白亚基基因（HMW-GS）上游Prolamin-Like box的结合特性,为小麦HMW-GS表达调控机制的阐明奠定基础。【方法】利用RT-PCR扩增得到WPBF编码区,将其连接到pET-21a-MBP载体中,并分别在E.coli T7 Express和Origami B（DE3）菌株中诱导表达。依次利用Amylose和Ni-NTA亲和层析柱纯化重组蛋白,最后通过凝胶迁移阻滞试验（EMSA）对重组蛋白的DNA结合特性进行分析。【结果】在33℃的Origami B（DE3）菌株中表达含MBP和His双标签的重组WPBF大部分以可溶性形式存在,其表达量占细菌总蛋白的48.0%。重组WPBF和含HMW-GS上游Prolamin-Like box的两类DNA探针能发生特异结合,但重组WPBF和含“TGCAAAG”基序DNA探针的结合强度高于其和含“TGCAAG”基序DNA探针的结合。【结论】建立了优化的WPBF原核表达系统,并获得具有生物活性的重组蛋白;重组WPBF能与HMW-GS启动子来源的两种Prolamin-Like box特异性结合, 但结合能力存在差异。     

核因子Y在植物中的分类及其功能概述

核因子Y(NF-Y)是近十年来新发现的具有多种生物功能的植物特异转录因子,由三个不同的亚基组成,分别是NF-YA(CBF-B/)HAP、NF-YB(CBF-A/HAP3)和NF-YC(CBF-C/HAP5)。NF-Y是一种典型的通过与调控因子相互作用来调控下游基因表达的转录因子,通过保守专一性的序列与真核生物启动子区域的CCAAT框相结合。NF-Y在植物中普遍存在,并参与调节植物体的诸多重要生理过程,包括逆境反应,胚胎和叶绿体发育,光合作用等重要生理过程。文章主要就植物NF-Y转录因子的基本结构特征、生物学功能、研究方法及进展等进行了综述。     

箱涵进海路结构的设计研究

箱涵进海路结构是一种适用于软土海底地基上的轻型海上路基结构,具有承载能力较好,施工方便快捷的特点。本文结合埕海油田张东开发海工工程,对箱涵进海路结构的抗滑、抗波浪浮托、抗倾稳定稳定进行了计算,计算结果表明箱涵进海路结构的稳定满足要求,为箱涵进海路结构的应用提供了理论上的保证。     

脉冲供水虹吸式滴灌控制装置

为使自压式滴灌系统省水、滴头抗阻塞，在自压式滴灌系统中安装了一种新研制的具有主箱和副箱相结合的脉冲供水虹吸式滴灌控制装置．经过初步试验，证明该装置能满足使用要求，对所灌溉地块大小变化适应性强，便于制造、使用和推广．该文分析和研究了该装置的结构和工作原理，并进行了水力计算．     

木质音箱的数控加工

为充分利用木材的天然特性,科学高效地使用木质材料,提高木质产品附加值,本试验应用计算机辅助设计与制造(CAD/CAM)和数控加工技术探讨了复杂木质音箱的加工生产特性.试验根据产品结构要求,绘制三维模型,进行数控编程及数控加工,并对加工工艺进行优化设计.研究结果表明,CAD/CAM和数控加工技术可高效地生产高质量的木质产品.     

塑盘营养土旱育与湿播旱管育秧对比试验

塑料营养土旱育秧虽能培育壮秧 ,但程序繁 ,技术难掌握 ,特别是大面积实施商品化育秧 ,在苗床地的选择、整做、营养土配制、播种、装盘、压盘等方面工序多。为简化操作 ,经试验在播种环节采用湿播法 ,播后采用旱育管理方式 ,表现出省工易操作 ,秧苗素质比较理想 ,这为较大面积商品化育秧打下了良好的技术基础     

The function of Hoxa-10 gene in the reproductive system

Hoxa gene plays an essential role in the generation and development of the human female reproductive system. This gene is expressed in the Mullerian duct and the adult female reproductive system. Expression of Hoxa10 dramatically increased during the midsecretory phase of the menstrual cycle, corresponding to the time of implantation. Female mice lacking Hoxa10 have a uterine factor defect that results in death of the preimplantation embryo and failure of implantation. These results suggest Hoxa10 may have an important function in implantation. Hoxa10 - deficient males exhibit cryptorchidism that lead to male infertility.     

教学实验室通风柜排风系统设计的思考

针对设计教学实验室通风柜排风系统中遇到的问题进行了探讨,并提出了设计中应注意的事项。