Analysis of Differential Shrinkage Deflection for PC Box girder Bridges

The long term deflection behavior of long span continuous rigid frame bridges has often attracted the attentions in engineering field. Based on the moisture diffusion theory, the calculation method of differential shrinkage deflection was proposed for PC box girder bridges. Finite Element Method(FEM) model of 2 D moisture field were established for typical cross sections of a case bridge. And the distribution law and the time variation characteristics of moisture field were analyzed. Through the deformation coupling condition of moisture field, the drying shrinkage deflection of the bridge was calculated and analyzed. It was found that the differential shrinkage deformation was significant and great error would be with the conventional method. When considering the effect of differential shrinkage, the deflection in mid span was 3.1cm larger than that of the conventional method. Thus, the differential shrinkage was the main reason for long term deflection of long span continuous rigid frame bridges.     

Astaxanthin Mitigates Thiacloprid-Induced Liver Injury and Immunotoxicity in Male Rats

Thiacloprid (TCP) is a widely used neonicotinoid insecticide with a probable toxic hazard to animals and human beings. This hazard has intensified the demand for natural compounds to alleviate the expected toxic insults. This study aimed at determining whether astaxanthin (ASX) could mitigate the hepatotoxic effect of TCP and diminish its suppressive effect on immune responses in rats. Animals received TCP by gavage at 62.1 mg/kg (1/10th LD₅₀) with or without ASX at 40 mg/kg for 60 days. Intoxicated rats showed modulation of serum transaminases and protein profiles. The hemagglutination antibody titer to sheep red blood cells (SRBC) and the number of plaque-forming cells in the spleen were reduced. The cell-mediated immunity and phagocytosis were suppressed, while serum interleukins IL-1β, IL-6, and IL-10 were elevated. Additionally, malondialdehyde, nitric oxide, and 8-hydroxy-2′-deoxyguanosine levels were increased in the liver, spleen, and thymus, with depletion of glutathione and suppression of superoxide dismutase and catalase activities. The expressions of inducible nitric oxide synthase and the high mobility group box protein 1 genes were upregulated with histomorphological alterations in the aforementioned organs. Cotreatment with ASX markedly ameliorated the toxic effects of TCP, and all markers showed a regression trend towards control values. Collectively, our data suggest that the protective effects of ASX on the liver and immune system of TCP-treated animals depend upon improving the antioxidant status and relieving the inflammatory response, and thus it may be used as a promising therapeutic agent to provide superior hepato- and immunoprotection.     

麦金属硫蛋白基因对酵母FD236-6A的转化及其对砷离子抗性的影响

金属硫蛋白(Metallothionein简称MT)可通过半胱氨酸残基的巯基与金属离子结合形成无毒或低毒的络合物,从而消除重金属的毒害作用。为研究绊根草二型金属硫蛋白CdMTII络合AsO33-的能力强弱及其具有的特性,把目的基因转化到模式生物酵母中,通过酵母转化体表现的抵抗重金属的能力来检测目的基因的功能强弱,为该基因转化到植物体中培育可用于植物修复的工程植物品种以及在医学等其它领域的应用奠定基础。     

矮牵牛pMADS4基因的克隆及序列分析(英文)

利用RT-PCR方法从矮牵牛(Petunia hybrida)中克隆了MADS盒基因pMADS4,进行了全序列测定.测序结果显示,所得到的基因与发表的序列同源率为100%.该基因长度为910 bp,含有一个开放阅读框,编码253个氨基酸残基组成的蛋白,具有典型的植物MADS盒基因的结构,该蛋白序列与玉米(Zea mays)ZAG3、麻竹(Dendrocalamus latiflorus)DIMADS18和拟南芥(Arabidopsis thaliana)AGL6的同源性分别为64%、64%和61%,说明pMADS4是AGL6在矮牵牛中的同源基因,推测它们具有相似的功能,都促进开花并且调节花器官形成.进化树分析显示,AP1/AGL9亚家族分为SEP、AGL6和AP1三个分枝,且AGL6分枝又进一步分为三组,分别与裸子植物、单子叶植物和双子叶植物类群相对应,预测通过分离植物的AGL6同源基因可以准确的判断植物所属类群.通过蛋白预测说明pMADS4蛋白以同源或异源二聚体的形式存在,具有结合DNA的功能.     

辣椒WRKY转录因子候选基因的瞬间表达分析

从辣椒cDNA文库中克隆获得一个推定的WRKY（CaWRKY）转录因子,为分析其功能,分别构建启动子区含有双倍W（2W）盒的报告载体和WRKY置于2x35S启动子控制下的超表达载体,通过基因枪瞬间转化技术将两种载体共转化洋葱表皮。检测共转化细胞中GUS基因的表达情况来分析转录因子与特定顺式作用元件的结合及对其转录的激活效应。结果表明CaWRKY能与W盒结合,表明该基因是WRKY转录因子的编码基因。     

基于ANSYS的某柴油机连接箱减重优化分析

对某柴油机连接箱的结构减重进行了研究。利用Creo软件建立连接箱模型并导入ANSYS中进行初始强度分析并校核,对模型进行拓扑优化分析,根据优化结果对模型进行合理的修改。对修改后的模型进行强度分析,并对比优化前后模型强度,以验证修改后的模型是否达到设计要求。结果表明,因合理改变连接箱结构,使得连接箱静力学分析结果有了较大的改善,对比分析显示,其应变值降低了44%,应力值下降了18.1%,减重10.9%,达到预期效果。     

《长恨歌》札记两则

白居易《长恨歌》中有两处细节值得讨论:一是"珠箔银屏迤逦开"句中的"银屏"指多扇联屏,"迤逦开"指"折叠";二是"钿合金钗寄将去"中"钿合金钗"反映了唐代上层社会流行的习俗:赠送情人以表达爱意.     

WTO框架下粮食价格保险政策归属及其改进

通过目标价格保险解决粮食托市收购政策带来的财政压力大、贸易扭曲等问题是当前我国各级政府及学者重点关注的农业价格改革方案之一。以黑龙江省庆阳农场的目标价格保险条款为例展开分析,研究发现,仅覆盖产量风险的粮食目标价格保险属于WTO"黄箱"政策的范畴。对比分析江苏常州实为收入保险的"目标价格保险"条款,提出将粮食目标价格保险政策调整为涵盖粮食价格风险及产量风险的区域收入保险政策,此政策归属WTO"绿箱"政策,可以在减缓目标价格支持政策困境的同时,减轻WTO"黄箱"政策支持总量上限压力。为更好地分散农业风险、保障农户收入,提出以下政策建议:建立覆盖价格风险以及产量风险的区域收入保险制度;建立农业保险与农业补贴互补的农户收入保障机制;完善粮食收入保险参照价格建立标准。     

高寒灌区风沙吹蚀对农业水利工程混凝土抗冻耐久性的影响

针对内蒙古引黄灌区农业水利工程混凝土实际服役环境,同时加大风积沙资源化利用效率,配制满足农业水利工程设计要求的风积沙混凝土,并研究风沙吹蚀作用对风积沙混凝土抗冻耐久性的影响。结果表明:相对动弹性模量可准确表征风积沙混凝土受风沙吹蚀影响下的冻融破坏,且风沙吹蚀影响下混凝土冻融循环后的内部损伤是未受风沙吹蚀影响下混凝土冻融循环后的2倍;在风沙吹蚀影响下的冻融过程中,风积沙混凝土水泥浆体在冻融过程发生"酥化",同时在风沙流的持续撞击、削切下,"酥化"的水泥浆体进一步剥落,迫使破坏表面水泥浆体与骨料连接处的界面过渡区较光滑;风积沙替代率为40%可以配制满足抗冻性要求的风积沙混凝土,但风积沙混凝土气泡间距系数不能准确评价其抗冻性,气泡盒维数与细骨料细度模数可反映气泡结构特征和细骨料颗粒级配,且二者呈负相关趋势,二者结合可对风积沙混凝土抗冻耐久性优劣进行初步判定。     

紫斑牡丹越冬防寒技术研究

通过对黑龙江省森林植物园引种的紫斑牡丹进行牛皮纸包裹法、草片包裹法、棉花包芽法、覆地膜法、涂白法、整体防寒法、防冻液涂干法、纸箱包裹法等9种防寒技术试验,结果认为纸箱包裹法保护的植株越冬后枝条存活率达95%以上,且防寒成本低,便于操作,建议推广使用。