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21.
本文根据微波与物质的相互作用情况,用微波照射舞毒蛾虫卵和幼虫,观察照射后虫卵孵化率和幼虫的死亡率的变化情况,目的是为物理辐照灭虫的研究和实际应用提供理论依据。实验表明:随着微波辐照剂量增大孵化率有明显下降趋势,而幼虫的死亡率有明显上升的趋势。 相似文献
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Summary Developmental stages during gametogenesis of rice were histologically examined in the period from differentiation of reproductive organs to anthesis. Plants were exposed to acute X-rays of 20 Gy. Radiosensitivity and mutation frequency were investigated in relation to the developmental stages of reproductive organs. The most radiosensitive stage, as measured by reduction of the M1 pollen-and seed-fertilities, was the last premeiotic interphase. Mutations induced at different developmental stages were scored in M3 strains. Sterility mutants and short-culm mutants were most frequently observed. Grain shape, panicle morphology, heading-date and endosperm character mutants were induced at a relatively low frequency. The overall mutation frequency varied with the developmental stage at the time of irradiation. The highest overall mutation frequency was observed when radiation was applied 10 days before anthesis, the late tetrad stage of microspores. Radiation exposure of florets at the late tetrad stage was found to be a more efficient method of inducing a large number of mutations than radiations applied to seeds and fertilized egg cells. 相似文献
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A plant with drastically reduced vigour was observed in a population of diploid barley (Hordeum vulgare) raised from seeds exposed to gamma radiation. Cytological studies revealed that the plant was nullisomic with 2n = 12. At meiosis, regular formation of 6 bivalents was observed. The plant was totally sterile and produced neither stainable pollen nor seed. 相似文献
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辐照杀虫是利用电离辐射与害虫的相互作用所产生的物理、化学和生物效应,导致害虫不育或死亡的一种物理防虫技术.用于辐照杀虫处理的射线主要是γ射线(60Co或137Cs)、10 MeV以下电子束以及5 MeV以下的X射线,其中钴源辐照杀虫产业化发展迅速,电子束和X射线辐照发展相对滞后,随着近年来加速器技术的进步,电子束辐照杀虫显示出独特的优势,并受到越来越广泛的重视.辐照对不同储粮害虫、同一种储粮害虫的不同发育时期和性别有着不同的生物学效应,总体来看储粮害虫对辐照较为敏感:不同害虫的辐射敏感性顺序为:鞘翅目>蜱螨目>鳞翅目;同种害虫不同虫态的敏感性顺序为:卵、幼虫>蛹>成虫;雌性害虫的敏感性大于雄性害虫.粮食辐照杀虫工艺流程一般包括:辐照前处理、虫情调查、剂量决策、辐照处理、辐照后管理和重复辐照等几个部分.在辐照杀虫的工艺剂量(0.3~0.5 kGy)条件下,辐照对粮食的食用品质和功能特性没有影响,辐照后的粮食也不存在卫生安全性问题.因此,辐照杀虫技术作为一种安全有效的储粮害虫防治手段,是储粮化学药剂的一种重要补充和替代,在储粮害虫防治领域有着巨大的市场前景. 相似文献
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Effects of gamma irradiation on in vitro pollen germination of different Cucumis species 总被引:6,自引:0,他引:6
Summary Pollen grains of 14 Cucumis accessions were irradiated with 0, 1, 2, 3 or 4 kGy acute gamma rays and germinated in vitro directly afterwards. Pollen germination was significantly reduced by increasing irradiation dose for all species, except C. melo var. agrestis. Pollen tube growth was generally reduced likewise. Pollen of two C. anguria subspecies was most sensitive to irradiation. Sensitivity of the pollen with respect to pollen tube growth in relation to irradiation dose was inversely related to total DNA amount per nucleus. In vitro germination was not related to DNA amount per nucleus. Results show that the examined Cucumis species, especially C. melo var. agrestis, are sufficiently resistant to irradiation to be used as donor species for in vivo egg cell transformation of the cucumber. 相似文献
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R. Pathirana 《Plant Breeding》1992,108(4):314-319
Due to the non-availability of sources of resistance in Sri Lanka to Phytopbthora blight in sesame (Sesamum indicum) caused by Phytopbthora nicotianae var. parasitica, a mutation breeding programme was initiated. Seeds of three genotypes were subjected to six doses of gamma rays from 100 Gy to 750 Gy from a 60Co source. Seeds of M2 bulks, sampled by variety and treatment from the first five capsules formed on M1 plants, were grown in a field with a history of repeated incidence of the disease. The best 21 lines, having the highest survival and seed production, identified in the screening of M3 and M4 progeny rows of selected M2 single plants were then tested in a replicated field trial with the recommended cultivar ‘MI 3’ as a control. The plant survival in selected lines averaged 43.3 percent as against 7.2 percent in the recommended variety. Eight selections recorded significantly higher seed yield than ‘MI 3’ at P < 0.01 and another three at P < 0.05. Gamma ray treatments of 450 Gy and 600 Gy produced more lines tolerant to the disease than the other doses used. 相似文献
28.
Summary Freshly cut leaves of Kohleria eriantha and K. x Longwood were exposed to a wide range of gamma irradiation doses and allowed to root and form adventitious buds. K. Eriantha could not be successfully propagated from leaf half cuttings. Longwood produced a small number of adventitious plantlets as compared to other Gesneriads. Colchicine treatments reduced leaf half survival in Longwood by more than 50%. Leaf halves exposed to low and moderate doses of gamma irradiation showed increased overall plantlet production compared to nonirradiated leaf halves.Of the mutation parameters calculated, the number of mutants per 100 surviving leaf halves appears to be the most useful since it relates the number of mutants within a dose to the number of surviving leaf halves, the number of mutants to the number of plants produced, and the number of mutants within doses to control values.Using the criteria, number of mutants per 100 surviving leaf halves, optimum production of all mutants, of useful and of dwarf mutants was obtained at 2.5 kR for noncolchicine treated leaf halves and 1.5 to 3.0 kR for colchicine treated leaf halves.Of the adventitious plantlets produced, 13.8% were classified as mutant types. Mutant plants were found in radiation dose levels up to 5.5 kR. The array of mutants produced was skewed toward plant habit and flower characteristic mutants with several leaf characteristic, lethal, and polyploid mutants observed. A high frequency of mutants, 10 of 93 plants, occurred in the nonirradiated controls. Four sectorial chimeras were identified from both control and irradiation exposed groups. Several potentially useful flower color and dwarf mutants have been selected for further study. Adventitious buds may have developed from callus at petiole and leaf-vein bases. Colchicine treatments severely inhibited adventitious plantlet production and because of this could not be used as a criterion to identify the origin of adventitious buds. Conclusive determination of the organogenesis of adventitious buds from Longwood leaf halves could not be made. Mutant plantlets formed from adventitious buds on detached leaf halves of Longwood appear to arise from single cells as has been found with other Gesneriads.Scientific Journal Series Article No. 10 330 of the Minnesota Agricultural Experiment Station. 相似文献
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