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101.
Hagemoser WA 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1989,18(1):17-20
An intravenous bile acid loading test was performed on 16 goats using various dosages and sampling protocols. A recently developed enzymatic bile acid assay provided an economical and technologically simple method for bile acid analysis. Intravenous injection of bile salts into goats was well tolerated. Clearance times were rapid. A protocol for performance of the test was suggested and a practical reference range for T 1/2 clearance of 4+/-1 minute was determined. Further investigations of this test in the assessment of liver function in the ruminant appear warranted. 相似文献
102.
103.
V Bitsch 《Acta veterinaria Scandinavica》1978,19(1):110-128
A study of the basic reaction in neutralization of virus (V) by virus-neutralizing antibody (VNA) was performed with infectious bovine rhinotracheitis virus and serum collected from naturally and experimentally infected cattle after the primary immunization phase. In constant-virus/varying-serum neutralization tests a direct proportionality between VNA titer and length of preincubation was observed and found to be in accordance with basic laws of neutralization. A deviation from this direct proportionality, which was partly attributed to the presence of a dissociable V-VNA complex, was seen with relatively short preincubation. Expressing a relationship between VNA titer, length of preincubation, and virus dose under conditions where a dissociable V-VNA complex can be ignored, a log. VNA/log. V equivalence factor of neutralization was introduced. A linear relationship was found between VNA titer, taken logarithmically, and preincubation temperature. A rise in temperature by 10°C gave an increase in VNA titer of approx. 1.2 in log2. Formulae are presented for the neutralization rate factor corrected for a demonstrated invalidity of the percentage law, and for the relation between the neutralization rate factor and VNA titer. It is concluded that the results presented have elucidated the possibilities of improving the sensitivity of neutralization tests. 相似文献
104.
Gülşen Sertkaya Marta Martini Paolo Ermacora Rita Musetti Ruggero Osler 《Phytoparasitica》2005,33(4):380-390
During the late summer-early autumn of 2002, surveys were carried out in Turkey to determine the presence of phytoplasma diseases
in fruit trees. Phytoplasmas were detected and characterized by PCR-RFLP analysis and TEM technique in stone fruit and pear
trees in the eastern Mediterranean region of the country. Six out of 24 samples, including almond, apricot, peach, pear and
plum, gave positive results in PCR assays. RFLP analysis usingSspI andBsaAI enzymes of PCR products obtained with primer pair f01/r01 enabled identification of the phytoplasmas involved in the diseases.
Stone fruit trees, including a local apricot variety (‘Sakıt’) and a pear sample, were found to be infected with European
stone fruit yellows (ESFY, 16SrX-B) and pear decline (PD, 16SrX-C) phytoplasmas, respectively. This is the first report in
Turkey of PD phytoplasma infecting pear and of ESFY phytoplasma infecting almond, apricot, myrobalan plum and peach; ESFY
phytoplasma infecting Japanese plum was previously reported.
http://www.phytoparasitica.org posting July 21, 2005. 相似文献
105.
106.
107.
在室内人工模拟干旱条件,测定了干旱胁迫下披碱草和野大麦及其杂种幼苗的相对含水量、细胞膜透性、游离脯氨酸含量的变化。结果表明,干旱胁迫下亲本披碱草幼苗的组织相对含水量下降的幅度最低,细胞膜受害最轻,游离脯氨酸积累的最多,相对生长率最高,表现出很强的抗旱性。亲本野大麦幼苗的组织相对含水量下降的幅度最大,细胞膜受害最重,游离脯氨酸积累的最少,相对生长率最低,抗旱性弱。杂种F1幼苗的保水能力、细胞膜受害程度和游离脯氨酸积累能力、相对生长率均介于双亲之间,亦即其抗旱能力大于野大麦而小于披碱草,表现为双亲的中间型。正、反交对杂种F1的抗旱能力没有明显影响。 相似文献
108.
模糊隶属法对玉米苗期耐旱性的拟合分析 总被引:14,自引:1,他引:14
在略高于玉米萎蔫系数的干旱条件下,以出苗率和生物学产量耐旱系数之乘积为指标,测定了17个玉米自交系和10个杂交种的苗期抗旱性。并用模糊隶属法,以干旱胁迫下的胚芽鞘长度,出苗率,根重,生物学产量,脯氨酸含量,电导率和离体叶片保水力等指标对各品种耐旱性进行了拟合分析。结果表明,在略高于萎蔫系数的干旱条件下,以出苗率和生物学产量的耐旱系数之乘积为指标,可以准确鉴定玉米苗期的耐旱性,与耐旱性的综合评价拟合良好(相关系数r=0.914)。 相似文献
109.
甘肃省河东地区主秋期降水量时空分布特征 总被引:3,自引:1,他引:3
利用甘肃省河东地区55个站30a的主秋期降水量资料进行经验正交展开(EOF)和旋转经验正交展开(REOF),分析其空间分布特征和异常类型;以平凉站为例分析了主秋期降水的时间变化规律和周期性;将河东地区主秋期降水量分为8个气候片。分析表明,河东地区主秋期雨量变化规律较为一致,空间分布不均,具有南多北少和东西多中间少的特点;多雨中心和少雨中心的多年平均值相差近200mm;主秋期降水年际变化较大,容易出现不同程度的旱或涝,并有9a左右的周期;20世纪40、60年代为相对多雨期,50、90年代为相对少雨期,70、80年代与平均值持平。 相似文献
110.
AIM:The β-catenin is a key molecule in the Wnt signal pathway, which plays a critical role in normal development and tumorigenesis. However, the mechanisms of the β-catenin on the cell growth control are still not completely defined. The aim of this study was to test the hypothesis that the mutant β-catenin may regulate the hepatocyte proliferation. METHODS: The immortalized murine hepatocyte cell line, AML12, was used for this study. A plasmid that contain mutant β-catenin S33Y was transfected into the AML12 cells and a stable cell line AML12S33Y was established. The cell growth property of this cell line and the parental cell were compared by flow cytometry analysis and direct cell count. The cells were also tested for the ability to form soft agar colonies, and the ability to form tumors in the severe immune deficient mice (SCID). RESULTS:1. The mutant β-catenin containing cell line AML12S33Y has higher proliferating index compared with the parental AML12 cells (P<0.01), suggesting that mutant β-catenin promotes cell growth. 2. The mutant β-catenin cells formed small colonies in soft agar after 4 weeks of culture, but did not generate tumor in SCID mice. CONCLUSION:The mutant β-catenin promotes liver cell growth. 相似文献