上流式厌氧污泥床处理豆制品废水启动研究

用上流式厌氧污泥床（ＵＡＳＢ）处理豆制品废水，以阐明这种消化器启动的效果。研究结果表明，产气率，ＣＯＤ去除率和甲烷含量分别为３．０Ｌ／（Ｌ．ｄ），９３％，５８．４％。从第２６天至４６天的运转期间，ＵＡＳＢ平均ＣＯＤ负荷率、产气率，ＣＯＤ去除率，甲烷含量分别为５．２ｇＣＯＤ／（Ｌ．ｄ），３．５Ｌ／（Ｌ．ｄ），９０％，５９．４％。出水乙酸，丙酸浓度分别为１１１ｍｇ／Ｌ，９８ｍｇ／Ｌ，丁酸浓度不可测出。     

Correlation between progression of atherosclerosis accelerated by emotional stimulation and imbalance of SDF-1/CXCR4 in mice

AIM To observe effects of emotional stimulation on expression of stromal cell-derived factor-1（SDF-1） and CXC chemokine receptor 4 （CXCR4） in plasma, platelets, aortas, hippocampus and bone marrow of apolipoprotein E gene knockout （ApoE^-/-） mice, and to reveal the possible mechanism of the aggravated atherosclerotic plaque vulnerability by emotional stimulation. METHODS Thirty 8-week-old male ApoE^-/- mice were randomly divided into normal control group, high fat group, and emotional stimulation group. Ten 8-week-old inbred C57BL/6J mice served as blank control group. After 12 weeks of intervention, the serum levels of SDF-1 and CXCR4 were investigated by ELISA. The protein levels of SDF-1 and CXCR4 in platelets, aortas, hippocampus and bone marrow were determined by Western blot. The pathological damage of aortas was observed by oil red O staining. RESULTS Compared with blank control group, normal control group and high fat group, the mice subjected to emotional stimulation showed more serious atherosclerosis in aortas detected by oil red O staining, and increased levels of SDF-1 and CXCR4 in the plasma and aortas were also observed （P<0.05）. The results of Western blot showed that the protein levels of SDF-1 and CXCR4 in platelets, aortas and hippocampus were increased in the mice subjected emotional stimulation, but the expression of SDF-1 and CXCR4 in the bone marrow was decreased （P<0.05）. CONCLUSION Imbalance of SDF-1/CXCR4 may be the key target by which emotional stimulation accelerates the progression of atherosclerosis.     

芥蓝二氢黄酮醇4–还原酶基因BoaDFR的克隆与功能鉴定

以白花芥蓝‘四季粗条’为材料克隆了Boa DFR基因,Gen Bank登录号为MT472647,CDS序列长为1158 bp,编码385个氨基酸,与甘蓝型油菜、甘蓝和白菜等具有高度同源性。Boa DFR表达水平在芥蓝不同发育时期、不同器官中存在显著差异,随植株发育总体呈现先下降后上升的趋势,在幼嫩种子中表达量最高。芥蓝原生质体的亚细胞定位结果显示Boa DFR定位在细胞核上。构建了农杆菌介导的芥蓝瞬时过表达体系,将Boa DFR转入白花芥蓝‘四季粗条’和黄花芥蓝‘福州黄花’中,发现Boa DFR在两种芥蓝中均高水平表达,转基因植株叶片颜色明显变紫,花青素苷显著积累,总含量最高达461.43μg·g-1 FW,而野生型和转空载体芥蓝叶片中几乎检测不到花青素苷。通过HPLC在转基因植株叶片中共检测到8种花青素苷,均为矢车菊花青素苷,其中矢车菊–3–阿魏酰–丙二酰–槐糖苷–5–葡萄糖苷占比最高。     

Expression of cathepsin C and tumor necrosis factor-α in human coronary artery and their relationships with coronary heart disease

AIM To explore the possible mechanism of cathepsin C （CTSC） and tumor necrosis factor-α （TNF-α） in coronary heart disease （CHD） by detecting the protein expression of CTSC and TNF-α in human coronary artery tissue. METHODS The coronary artery tissues from 52 cases of CHD and 25 cases of accidental death without any heart disease in the Forensic Judicial Expertise Center of Guizhou Medical University from October 2018 to December 2019 were collected as CHD group and control group, respectively. The coronary artery stenosis and intimal plaque formation were examined by histopathology, the protein expression of CTSC and TNF-α was determined by Western blot, and the intracellular expression of CTSC and TNF-α was analyzed by immunohistochemical staining. RESULTS The results of HE staining showed that the intima of coronary artery in control group was smooth, and no thickening or stenosis was observed. In CHD group, the intima thickened irregularly, atherosclerotic plaques formed, the intima became thinner and the lumen showed eccentric stenosis in varying degrees （P<0.05）. The results of Western blot showed that the expression of CTSC and TNF-α in CHD group was significantly higher than that in control group （P<0.05）. Immunohistochemical staining showed that both CTSC and TNF-α were expressed in the cytoplasm of foam cells, and their positive expression was significantly higher than that in control group （P<0.05）. The results of Pearson moment correlation analysis showed that there was positive correlation between the expression of CTSC and TNF-α in CHD （r²=0.743, P<0.05）. CONCLUSION The up-regulated expression of CTSC in coronary artery tissue may promote the expression of TNF-α and affect the occurrence and development of CHD.     

高效实用型黄瓜嫁接技术——生长点斜插法

为了提升黄瓜嫁接苗对设施栽培连作障碍的耐受能力,提高幼苗的抗病性与抗逆性,同时确保嫁接成活率,增加产品产量及品质,在传统的黄瓜嫁接方法基础上,改进出高效实用的生长点斜插嫁接法,为此创新出了专用嫁接工具——β形铝合金嫁接签,并申报了实用新型专利（专利号： 201811595026.X）;为了提高嫁接苗的成活率,在对砧穗进行特定处理的同时,还探索出了嫁接苗生长最佳环境的保障装置——育苗钢架。通过育苗基质的营养控制、省略嫁接夹具的插接方法及保温保湿的育苗钢架等系列配套技术的使用,使得生长点斜插嫁接技术具备了突出的实用性与高效性,十分适合在实际生产中推广使用。     

吴茱萸嫁接方法试验

试验表明:5种嫁接方法苗木成活率均在80％以上,其中以贴枝接和长块削芽接为最高,平均成活率达95％以上,但从快速繁育苗木着眼,以长块削芽接为好。嫁接适期以3月中旬树液流动时为最好。嫁接苗当年在圃地就能开花结果。     

数据仓库逻辑建模的初步探讨

针对逻辑建模在数据仓库构建过程中的重要性,介绍了数据仓库创建的基本步骤,并利用实例深入而详细地研究了逻辑模型的设计,分析了几种逻辑模型的特点及其使用场合。     

JAZ蛋白介导的茉莉酸信号传递

当植物受到生物或非生物因素胁迫时,体内会合成茉莉酸类物质,启动自身的防御反应。茉莉酸途径的抑制因子JAZ(Jasmonate ZIM-domain)蛋白近期被确认,该文对JAZ蛋白在茉莉酸信号传递中的作用作一综述。     

兴安落叶松容器苗化学剪根效果与根生长潜力测定的研究

采用内壁涂有不同浓度碳酸铜的容器培育出兴安落叶松(Larix gmelini(Rupr.)Rupr.)苗木,苗木侧根受碳酸铜的抑制,减小了根系变形的程度。通过对处理苗木根生长潜力的测定表明:碳酸铜浓度为100～150g/L时改变了兴安落叶松容器苗新根的分布状态,根系中、上层的新根数量明显增加,这样的苗木根系更接近自然发育的苗木根系,从而克服了一般容器苗根系易变形的缺点。     

黑线仓鼠胴体重长指标的研究

黑线仓鼠（Ｃｒｉｃｅｔｕｌｕｓｂａｒａｂｅｎｓｉｓ）是华北平原最主要的农田害鼠之一，本文用胴体重代替体重采用数理统计方法，分析了其胴体重与体长之比（即胴体重长指标）的年龄、性别、季节、年度变化，结果除年度变化外，均发现有显著性差异，雄性的该值较雌性的大得多，同性相比，Ⅳ龄组最大，在１９８７１９８９年间的种群密度下，其胴体重长指标与种群密度间没有相关关系