基于土壤水分下限的宁夏枸杞滴灌灌溉制度试验研究

以宁杞7号枸杞为研究对象,在宁夏同心县开展2 a田间试验.设置4个关键控水期、3个控水水平共8个处理,研究枸杞不同生育期不同水分下限条件下根区土壤含水率、叶片光合生理指标、产量与品质变化;分析其耗水规律与水分利用效率,提出基于土壤水分下限的宁夏枸杞滴灌灌溉制度.结果表明:不同水分处理枸杞根区20~60 cm土层土壤含水率最大;叶片气孔导度随土壤下限升高而增大,高水分下限处理的蒸腾速率相对较大,叶片水分利用效率则相反;生育期耗水量随土壤水分下限升高而增大,2 a增幅分别为10.8%和12.8%,平均耗水量为386.6~463.2 mm,夏果期耗水量最大且差异具有统计学意义,是关键需水期.2 a均为处理S5的产量最大分别为2 208.15和2 571.30 kg/hm²,水分利用效率最高为0.39 kg/m³;水分处理对蛋白质含量影响差异不具有统计学意义,低土壤水分下限的枸杞多糖含量相对较高;全生育期分为6个灌水期,其中萌芽期灌水(春水)为375 m³/hm²;春梢生长期、花期、夏果期、秋果期的土壤质量含水率下限分别为50%θ_f(θ_f为田间持水率),65%θ_f,65%θ_f,55%θ_f,而上限为95%θ_f;休眠期冬灌量为450 m³/hm².     

植物免疫诱导剂在草莓中的应用情况

为了助力实现草莓的安全生产,对一类能诱导植物产生免疫防御反应的植物免疫诱导剂在草莓中的应用情况进行综述。主要阐述了植物免疫诱导剂的种类、诱导机理及其在草莓栽培上的应用现状,发现:植物免疫诱导剂能促进草莓生长和发育、诱导抗病性、提高产量、改善果实品质、提高耐贮性等,从而减少了化学农药的使用,有利于草莓高质量生产,应用前景广阔。     

The roles of light and pericarp on seed dormancy and germination in feral Raphanus sativus (Brassicaceae)

The timing of seed germination may determine the success of a weed species in an agroecosystem, and its expression is modulated by environmental conditions, but also by seed physiology and anatomy. The aims of this study were to investigate the roles of light, pericarp, dry storage and cold stratification on seed dormancy and germination in feral radish, a troublesome agricultural weed in temperate zones of the Americas that reduces crop yields. To this end, we used isolated intact pods and extracted seeds to test germination over time under contrasting temperature, light and storage conditions. Here, we showed that fresh seeds were non‐dormant, but that light and the presence of the pericarp reduced germination, especially under low temperatures. The pericarp reduced the final water content absorbed by seeds inside pods and decreased absorption/dehydration rates. The pericarp showed several small lignified cell layers in the endocarp, and x‐ray images displayed the lack of space between the partially embedded seed and the endocarp. Dry storage and cold stratification were ineffective in breaking the dormancy imposed by the pericarp. The apparent requirement for darkness and the mechanical restriction of the pericarp may have the potential to induce dormancy, spreading the timing of seed germination over a more extended period and hindering the control of feral radish.     

Zerumbone attenuates MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells by inhibition of oxidative stress

AIM: To investigate the role of zerumbone (ZER) in 1-methyl-4-phenylpyridinium (MPP⁺)-induced cytotoxicity of human neuroblastoma SH-SY5Y cells. METHODS: Human neuroblastoma SH-SY5Y cells were cultured in vitro and the protective effect of ZER against MPP⁺-induced cytotoxicity was measured by CCK-8 assay. Flow cytometry was used to determine the apoptosis and reactive oxygen species (ROS). The expression of Parkinson disease protein 7 (PARK7) was knocked-down by using PARK7-specific short hairpin RNA (shRNA). The protein levels of PARK7, nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot. RESULTS: MMP⁺ remarkably reduced the cell viability in a dose-dependent and time-dependent manner. The SH-SY5Y cell injury model was established by treatment with MPP⁺ at 600 μmol/L for 24 h. ZER up-regulated the protein levels of PARK7 and Nrf2 (P<0.05), alleviated apoptosis (P<0.05), and reduced ROS production (P<0.05) in the SH-SY5Y cell injury model. Meanwhile, N-acetyl-L-cysteine (NAC) had the similar functions. Moreover, significant reductions in the protein levels of Nrf2 and HO-1 (P<0.05), and obvious increases in apoptosis (P<0.05) and ROS level (P<0.05) were demonstrated in PARK7-knockdown cells. CONCLUSION: ZER protects SH-SY5Y cells against MPP⁺-induced cytotoxi-city, which may be related to activation of PARK7/Nrf2/HO-1 pathway, and subsequent attenuation of oxidative stress and apoptosis.     

Do cover crops change the lability of phosphorus in a clayey subtropical soil under different phosphate fertilizers?

Plants have developed different mechanisms to absorb and solubilize phosphorus (P) in the soil, especially in environments with low P availability. This study evaluated the effects of different winter cover crops on soil P availability in a clayey subtropical (Hapludox) soil receiving soluble P fertilizer and a rock phosphate applied to the summer crop, under no‐tillage. The experiment was carried out over 3 yrs (2009–2011) with five different cover crop species: common vetch, fodder radish, ryegrass, black oat, white clover and fallow as control. The soil was sampled after the third year of cover crop cultivation and analysed for inorganic and organic P forms according to the well‐established Hedley fractionation procedure. Phosphate fertilizers promoted accumulation of both labile and nonlabile P pools in soil in the near surface layer, especially under rock phosphate. Fertilizer applications were not able to change P fractions in deeper layers, emphasizing that the Brazilian clayey soils are a sink of P from fertilizer and its mobility is almost nil. Although the cover crops recycled a great amount of P in tissue, in a short‐term evaluation (3 yrs) they only changed the content of moderately labile P in soil, indicating that long‐term studies are needed for more conclusive results.     

全国水土保持功能年度价值评价研究

依据《中华人民共和国水土保持法释义》对水土保持功能的定义,分析了水土保持设施、地貌植被所发挥或蕴藏的有利于保护水土资源、防灾减灾、改善生态、促进社会进步等作用的内涵。在借鉴国内外先进经验和研究成果基础上,结合我国水土流失特点、水土保持现状,界定出水土保持功能价值概念。采用生态经济学计量方法,结合实际调研情况,分析各个指标之间的逻辑关系,建立了水土保持功能价值评价模型。按照国家水土保持区划一级分区,适当考虑二级分区和三级分区,以及全国水土流失动态监测站网分布概况,收集39个典型县(市、区)2016年度的土壤、水文、气象等观测资料,测算出其水土保持功能价值,进而推算至全类型区,再从8个水土保持一级分区的水土保持功能价值推算出2016年度全国水土保持功能价值,并在对测算结果进行分析讨论的基础上,对国家制定水土保持生态文明对策提出了建议。     

基于森林健康理念的森林康养产业发展研究

阐述了森林健康理念和森林康养的基本概念,介绍了森林健康理念和森林康养的发展现状,分析了森林健康与森林康养产业之间的关系。结果表明:森林康养产业的理论基础就是基于森林健康理念继承发展来的,必然会促进森林健康产业的发展。运用森林健康理念指导森林康养产业发展是森林康养产业可持续发展的有效途径,森林健康理念在中国会不断完善和发展,也更有利于中国森林康养产业的发展。展望康养产业,会形成一种新的经济形态,成为国民福祉。     

苹果花脸病田间病情发展及ASSVd在组培条件下的传播

为了明确苹果锈果类病毒（apple scar skin viroid，ASSVd）引起的苹果花脸病在田间的病情发展情况以及ASSVd在组培条件下的传播方式，2012—2015年对河北省顺平南神南村3个果园进行持续调查和检测。结果显示，果园A、B、C的显症率分别从3.00%、19.35%和10.00%上升至10.00%、34.41%和22.00%，带毒率分别从11.00%、20.43%和14.00%上升至23.00%、37.63%和30.00%，带毒率和显症率都逐年增加，而且带毒率大于显症率，即有些带毒果树不显症。发病果树田间分布有明显的发病中心。通过高通量测序发现显症果树ASSVd vsiRNA reads数是带毒未显症reads数的4.08倍，同时实时荧光定量PCR检测发现显症树ASSVd病毒积累量显著高于带毒未显症树。以携带ASSVd的组培苗和无毒组培苗为试材，利用RT-PCR检测明确了ASSVd可通过伤口沾染带毒汁液、组培剪污染、含毒培养基、根系接触进行传播，其中根系接触传染率较高。     

不同氮素水平下双季稻株型与冠层内光截获特征研究

本文旨在阐明双季稻株型与冠层内光合有效辐射截获的时空分布特征。选用4个不同株型早、晚稻品种,设置4个不同施氮水平,系统观测其植株形态和冠层内光合有效辐射截获率(IPAR)的时空分布状况。结果表明,施氮水平对早、晚稻株高、穗长、叶长和叶基角均有显著影响,均表现为随施氮水平的增加而增大;早、晚稻孕穗期的分层叶面积指数(LAI)和向上累积LAI大于抽穗后12 d,分层LAI呈冠层中部大于上部和下部的分布特征,最大分层LAI出现在0.58相对高度处;冠层上中部分层LAI和向上累积LAI随施氮水平的增加而增大;向上累积LAI随相对高度呈S型曲线分布,可用Logistic方程定量描述(R~2 0.99);早、晚稻孕穗期的冠层IPAR大于抽穗后12 d,且随施氮水平的增加而增大,其日变化表现为正午较小,早晚较大;株型紧凑的早、晚稻品种,冠层IPAR低;冠层IPAR与向下累积LAI之间的关系可用方程IPAR=a (1-e~(-b×LAI))定量描述(R~2 0.88);冠层内IPAR的三维空间分布表现为冠层上中部水平面上IPAR较低,光斑变化大,冠层下部水平面上IPAR较高,光斑变化较平缓,同一冠层高度水平面上的IPAR呈不均匀分布。研究结果可为双季稻高产栽培及理想株型的优化设计提供支撑。