皮下及造血组织坏死杆状病毒对中国对虾亲虾的人工感染

皮下及造血组织坏死杆状病毒对中国对虾亲虾的人工感染宋晓玲，黄倢，王崇明，于佳，陈碧鹃，杨丛海（黄海水产研究所，青岛２６６０７１）关键词中国对虾，亲虾，皮下及造血组织坏死杆状病毒，人工感染，温度效应ＡＲＴＩＦＩＣＩＡＬＩＮＦＥＣＴＩＯＮＯＦＢＲＯＯＤＳ...     

Taxonomic and functional macroinvertebrate diversity of high-altitude ponds in the Macun Cirque,Switzerland

1. Global environmental change is threatening freshwater biodiversity with ecological impacts predicted to be particularly severe in high-altitude regions. Despite this, an ecological understanding of high-altitude pond networks remains patchy, with only limited knowledge of the environmental and spatial predictors of taxonomic and functional diversity. Moreover, previous studies of pond ecosystems have focused primarily on taxonomic richness and largely overlooked functional diversity.
2. This study examined the influence of local environmental and spatial factors on taxonomic and functional α and β diversity (including the turnover and nestedness-resultant components) of 17 high-altitude (~2,500 m above sea level) pond macroinvertebrate communities, in the Macun Cirque, Switzerland.
3. Spatial processes (pond connectivity) were important drivers for taxonomic α diversity, while local environmental variables (pond permanence and surface area) were important determinants of functional α diversity. Species turnover was the most important component of β diversity for taxonomic composition, and functional composition demonstrated a nested spatial pattern.
4. Variation in taxonomic and functional composition (and the turnover and nestedness components of β diversity) were determined by local environmental variables despite the limited environmental gradients within the pond network. No significant effects of spatial variables on community composition were recorded for either facet of diversity, indicating that compositional variation was determined at a local scale. Water temperature, depth and pond permanence were consistently the most important measured drivers of diversity.
5. Given the importance of both spatial and environmental variables in structuring taxonomic and functional diversity, landscape-scale conservation and management activities that aim to improve or protect high-altitude freshwater biodiversity should focus on maintaining connectivity among ponds and environmental heterogeneity, particularly pond surface area, water depth, and hydroperiod. Understanding the mechanisms driving taxonomic and functional diversity will be critically important for the management and conservation of macroinvertebrate communities in high-altitude pond networks in the face of climatic warming.

     

Litopenaeus vannamei (Boone) post-larval survival related to age, temperature, pH and ammonium concentration

Transport of post‐larvae shrimp used in aquaculture is an important element of successful cultivation because of the potential for stress during stocking procedures. To find optimum transport conditions, several bioassays were performed in the laboratory to evaluate survival of whiteleg shrimp Litopenaeus vannamei 5–30‐day‐old postlarvae under conditions similar to those encountered during transport from the hatchery to nursery and shrimp ponds. Postlarvae were exposed for 4 h to different temperatures and pH levels ammonia concentrations. Survival was significantly reduced after a 4 h exposure to pH 9 and was inversely related to temperature with or without 7 mg L^?1 of ammonia. The 15‐ and 20‐day‐old postlarvae had higher survival rates than other ages. The lowest survival occurred in alkali conditions (pH 9), with 7 mg L^?1ammonia at 30 and 32°C. To assure optimal survival of postlarvae during transfer from the hatchery to the nursery and shrimp ponds, we recommend temperatures below 28°C, pH no higher than 8, no ammonia and post‐larval age at least 15 days.     

Intake and discharge nutrient loads at three intensive shrimp farms

This study was conducted to help provide a framework for Australian regulation of shrimp farm siting and discharges. Monitoring of farm water usage, and intake and discharge water quality was conducted at three commercial intensive shrimp farms, chosen to represent different operating environments, latitudes, cultured species and management styles. Weekly samples were taken over 3 years, for 3–12 months at each farm, to investigate intake and discharge concentrations and loads of total suspended solids (TSS), total nitrogen (TN) and total phosphorus (TP). Mean water exchange was 1.4 ML ha^?1 day^?1 (about 10% day^?1) at the first farm studied and 0.5 ML ha^?1 day^?1 (about 3.6% day^?1) at the others. Farm mean discharge concentration varied as follows: TSS, from 36.9 to 119 mg L^?1; TN, from 2.1 to 3.1 mg L^?1 and TP, from 0.22 to 0.28 mg L^?1. Farm mean intake concentrations were from 11% to 91% of equivalent mean discharge concentration (for TN at Farm B and TSS at Farm C respectively). Mean net discharge loads, related to area of production ponds at each farm, varied as follows: TSS, from 4.8 to 85.7 kg ha^?1 day^?1; TN, from 1 to 1.8 kg ha^?1 day^?1 and TP, from 0.11 to 0.22 kg ha^?1 day^?1. The highest net loads of TSS, TN and TP were all from the farm with the highest water exchange rate, located on a coastal river, and studied during a year of high rainfall with associated poor water quality. These results can be used to help predict likely discharge characteristics for new shrimp farms, and provide a benchmark against which to evaluate future improvements in shrimp farm environmental management.     

Characterization of intake and effluent waters from intensive and semi-intensive shrimp farms in Texas

Two commercial shrimp farms in south Texas were evaluated for influent and effluent water quality from June to October 1994. The intensive farm, Taiwan Shrimp Village Association (TSV) had an average annual yield of 4630 kg ha^?1 while the semi‐intensive farm, Harlingen Shrimp Farm (HSF), had a yield of 1777 kg ha^?1. The study had three objectives: (1) to compare influent and effluent water from the intensive and semi‐intensive shrimp farms, (2) to show which effluent water‐quality indicators exceeded allowable limits, (3) to indicate inherent problems in farms operated with water exchange and summarize how findings from this study led to changes in farms' management that limited potential negative impact on receiving streams. Water samples were collected and analysed twice a week for the TSV farm and once a week for the HSF farm. Samples were analysed for dissolved oxygen (DO), salinity, pH, ammonia‐nitrogen (NH₃‐N), nitrite‐nitrogen (NO₂‐N), nitrate‐nitrogen (NO₃‐N), total phosphorus (TP), total reactive phosphorus (TRP), five‐day carbonaceous biochemical oxygen demand (cBOD₅), total suspended solids (TSS) and settleable solids (SettSols). Most of the effluent constituents showed fluctuations throughout the sampling period often related to harvest activity. Effluent pH at TSV was lower than influent values but within the regulatory requirements set by Texas Commission of Environmental Quality (TCEQ), formerly known as Texas Natural Resource Conservation Commission (TNRCC). HSF effluent pH values were higher than its influent, but still within TCEQ limits. Effluent DO mean levels were generally below the regulatory daily mean requirement, with values at TSV often below those for influent. Effluent nutrient concentrations and net loads were generally higher at the intensive shrimp farm, with NH₃‐N mean concentrations above the daily mean set by the TCEQ on several occasions. Effluent TSS concentrations were higher than influent for both farms, with daily mean values above the TCEQ limit. The two farms presented similar TSS concentrations despite their different stocking densities. However, TSS total net load and net load per hectare were higher at the intensive farm. The semi‐intensive farm presented higher cBOD₅ concentrations and net loads despite its lower stocking density, with daily mean values above the TCEQ limit. The cBOD₅ net load at TSV presented negative values indicating higher load at the influent than at the effluent. Analyses showed no evidence of self‐pollution between influent and effluent at the two farms. The high feed conversion ratio (FCR) values (2.3 and 2.7 for the intensive and the semi‐intensive farm respectively) suggest that better feed management is needed to reduce nutrient and solid net loads release from the two farms. The data obtained from this study resulted in several modifications in design and management of the two farms that reduced the potential negative impact on receiving streams. A brief summary of the improvement in selected effluent water‐quality indicators at the intensive shrimp farm is provided.     

Evaluation of shrimp polyculture system in Thailand based on stable carbon and nitrogen isotope ratios

ABSTRACT: To quantify the contribution by cocultured animals to waste assimilation in an intensive shrimp farm in Thailand, the food web structures of the macrobenthos in a reservoir pond, a shrimp culture pond and water treatment ponds were examined using the stable C and N isotope ratio technique. Seawater for aquaculture was drawn from a creek, and stored in a reservoir pond, used for farming the banana prawn Fenneropenaeus merguiensis in culture ponds, and then recycled through treatment ponds where the green mussel Perna viridis was cultured to remove organic wastes discharged from the farming. The clam worm Nereididae sp. and the mud creeper Cerithideopsilla cingulata in the culture pond had δ ¹³C values of −21.0‰ and −18.4‰, respectively, suggesting that shrimp feed (mean δ ¹³C = −20.7‰) was the main food source for these species. The δ ¹³C analysis also suggested that sediments (−23.7‰) in the reservoir pond and particulate organic matter (POM) (−24.0‰) and/or sediments (−25.0‰) in the treatment pond supplied carbon for most macrobenthic animals. However, green mussels in the treatment pond had a mean δ ¹³C value of −20.5‰, suggesting that shrimp feed was the main food source for this species.     

对虾池的放养密度对浮游生物群落的影响

于１９９８年７￣９月在山东海阳养虾场运用陆基围隔实验生态学方法,研究了对虾与罗非鱼在五种放养密度下浮游生物群落的变动情况。结果表明;可能浮游动物的摄食对浮游植物产生影响,二者数量的消长在大多数围隔都呈负相关;纤毛虫的数量与浮游植物的总生物量呈负相关;随着养殖密度的增加,浮游植物生物量减少,而浮游动物却有所增加,前者的优势类群由硅藻变为甲藻、蓝藻和隐藻。后者的在组成中镖水蚤数量减少而剑水蚤、无节幼体、轮虫和原生动物等小型种类有所增加;纤毛虫在高密度围隔中大量繁殖,说明水质已逐渐恶化。     

Apparent digestibility of selected ingredients in diets for white shrimp Penaeus setiferus L.

A study was conducted to determine apparent digestibility coefficients for dry matter (ADMD), crude protein (ACPD) and gross energy (AED) of selected ingredients in diets for juvenile Penaeus setiferus . Ingredients tested were corn grain, cottonseed meal, crab meal, grain sorghum, meat and bone meal, menhaden fish meal, rice bran, rice grain, shrimp meal, soybean meal, squid liver powder, wheat flour, wheat gluten, wheat middlings and wheat shorts. ADMD coefficients of test ingredients ranged from 22% to 100% and appeared to be related to the fibre, ash and starch content of the ingredient. ACPD coefficients ranged from 58% to 100%. Plant protein supplements (soybean meal and cottonseed meal) were utilized as efficiently as, or better than, animal protein supplements (squid liver powder, menhaden fish meal, meat and bone meal, shrimp meal and crab meal). ACPD coefficients for meat and bone meal, shrimp meal and crab meal (58–59%) were significantly lower than those for menhaden fish meal (76%) and squid liver powder (82%). AED coefficients (44–100%) varied significantly among plant products of similar proximate composition. Corn grain and wheat flour were better utilized by P. setiferus than other high-starch energy supplements. Wheat gluten exhibited the highest ADMD, ACPD and AED coefficients of all ingredients tested.     

Manufacturing Different Diet Sizes and Its Effect on Pellet Water Stability and Growth of Three Size Classes of Pacific White Shrimp,Litopenaeus vannamei

Abstract

A feed manufacturing method involving the production of large pellets followed by crumbling to desired diet sizes was used to produce six shrimp diets to determine its effect on growth of three size classes of Pacific white shrimp, Litopenaeus vannamei. The six diets containing 45% protein were 0.7 mm crumble, 1.2 mm crumble, 1.7 mm crumble, 2.2 mm crumble, 2.6 mm crumble, and 3.0 mm pellet. These diets were fed to three size classes of shrimp subjected to a four-week growth trial under indoor laboratory conditions. Stocking density was 12 shrimp/aquarium for size class 1 (1.13iö.01 g), 9 shrimp/aquarium for size class 2 (7.31iö.06 g), and 6 shrimp/aquarium for size class 3 (13.12iö. 10 g). Shrimp were fed three times daily and were evaluated for live weight, growth rate, feed conversion ratio (FCR), and survival. Results for shrimp growth appeared to be affected by diet size and pellet water stability. The effects of diet size, however, were more evident on smaller sized shrimp. Weekly growth rates for class 1 and class 2 shrimp indicated significant responses (P < 0.05) to the effects of diet size, while the class 3 shrimp did not show any significant result. The growth rates for both class 1 and class 2 shrimp were lowest at the smallest diet size (0.7 mm crumble) but differed with respect to highest growth rates. The largest diet (3.0 mm pellets) yielded the highest growth rates for class 1 shrimp, while medium sized feed (2.2 mm crumble) produced the best growth for class 2 shrimp. This could be partly attributed to pellet water stability, which had a direct correlation to diet size (r = 0.97). Smaller diet sizes resulting from crumbling larger pellets tend to have lower water stability. The water stability decreased from 82.5% to 76.3% as the 3.0 mm pellets were reduced to 0.7 mm crumbles. Stress cracks are created during crumbling, and surface area to volume ratio are increased as larger particles are reduced to crumbles. These would make smaller diet particles more susceptible to disintegration and nutrient leaching. For class 3 shrimp, the largest pellets produced the highest growth rate. This high growth value, however, was not significantly different (P > 0.05) from growth results produced by the crumbled feed sizes. This observation suggests that diet sizes ranging from 0.7 mm crumble to 3.0 mm pellet could be used for a 13 g shrimp, although preference would be given to larger pellets due to higher growth performance and better pellet water stability. Results for shrimp live weights were similar to results obtained for weekly growth rates. FCR and survival of all shrimp sizes did not show any significant response from the effects of various diet sizes. However, larger shrimp were less efficient in their diet utilization than smaller shrimp.     

亚热带水产养殖寒灾预警系统的设计与实现

通过对亚热带水产养殖现状的分析，论述了亚热带水产养殖寒灾预警系统建设的必要性、研究的目标和任务，详细介绍了系统的结构和框架，以及亚热带水产养殖寒灾预警的几个关键问题及其解决方法。