不同引物对植原体的检测灵敏度比较研究

用常规PCR和巢式PCR对目前常用的各种植原体引物(fPl/rP7、fU5/rP7、fU5／rU3、fAY/rEY和fFD9/rFD9)的检测灵敏度进行了比较研究。研究发现，它们的检测灵敏度并不相同。最灵敏的引物(fAY/rEY)要比最不灵敏的引物(fFD9/rFD9)的灵敏度至少高出数千倍。因此，按照不同的检测目的选择所用的检测引物是明智的，特别是当待测样品中的植原体浓度极低时，比如葡萄组织样品中植原体的检测。据此提出了用于不同检测目的时的最佳引物。     

猪胚胎死亡的影响因素及其预防措施

猪胚胎死亡直接影响着母猪的产仔数，并进而影响着养猪生产的经济效益。因此，在深入研究猪胚胎死亡规律及其影响因素的基础上，通过有效的措施降低胚胎死亡率，对提高母猪窝产仔数和经济效益具有十分重要的意义。本文综述了影响猪胚胎死亡的遗传、环境、营养和疾病等因素，并对生产实践中如何通过综合预防措施降低猪胚胎死亡率进行了初步探讨。     

圆点印迹法检测葡萄斑点病毒技术研究

采用圆点印迹法检测GFkV，结果表明：Tris-HCl和PBS在提取GFkV时效果相同，在带毒葡萄植株中韧皮部的GFkV含量较高。     

禽流感病毒夹心ELISA快速检测方法的研究

以禽流感病毒(AIV)湖北分离株(H9N2亚型)提纯物为免疫原，制备出鸡抗AIV和兔抗AIV抗体，经用琼脂扩散法测得效价分别为1:32和1:16。以纯化的鸡抗AIVIgG为包被抗体，兔抗AIVIgG为第二抗体，建立了检测AIV抗原的夹心ELISA法。结果表明，鸡抗AIVIgG的最佳包被浓度为1μg/mL，兔抗AIV IgG的最适工作浓度为5μg/mL；对已知的阳性样品，用夹心ELISA法测得的病毒滴度比血球凝集滴度高16倍以上，且能检出其它亚型的禽流感病毒；与新城疫病毒、传染性支气管炎病毒、减蛋综合征病毒、传染性喉气管炎病毒、传染性法氏囊病病毒、鸡痘病毒、马立克氏病病毒等无交叉反应，说明本方法有很高的特异性及敏感性。对14个鸡场送检的、患有呼吸道疾病或有腹膜炎、眼炎、产蛋下降、怀疑为禽流感感染的病鸡进行了检测，结果有7个鸡场为阳性。本方法的建立为禽流感病鸡群的临床检验提供了一种方便、敏感、快速的检测方法。     

数据密集型计算环境下的离群点挖掘算法

在数据密集型计算环境中，数据的海量、高维、分布存储等特点，为数据挖掘算法的设计与实现带来了新的挑战。基于MapReduce模型提出网格技术与基于密度的方法相结合的离群点挖掘算法，该算法分为两步：Map阶段采用网格技术删除大量不可能成为离群点的正常数据,将代表点信息发送给主节点；Reduce阶段采用基于密度的聚类方法,通过改进其核心对象选取,可以挖掘任意形状的离群点。实验结果表明,在数据密集型计算环境中,该方法能有效的对离群点进行挖掘。     

Evaluation of two commercial PRRSV antibody ELISA kits with samples of known status and singleton reactors

Two commercial PRRSV ELISA kits (IDEXX and Bionote) were evaluated for their sensitivity and specificity using 476 PRRS-positive serum samples collected from 7 animal challenge experiments and 1,000 PRRS-negative sera. Both ELISA kits exhibited 100% sensitivity with sera collected 14 to 42 days post-infection, and the results from the kits were highly correlated (R²=0.9207). The specificity of IDEXX or Bionote kit was 99.9% or 99.7%, respectively. In addition, the Bionote ELISA kit was used to examine 100 sera that were determined to be falsely positive either by IDEXX 2XR or 3XR ELISA, and only 7 of these samples were found to be positive. These results indicate that both ELISA kits exhibited similar levels of sensitivity and specificity and would complement one another for the verification of false-positive samples.     

2011年-2013年滨州及其周边地区4种猪病毒性疾病的流行情况分析

通过对滨州及其周边地区2011年-2013年316个场次采集病料进行猪瘟病毒、猪繁殖与呼吸综合征病毒、猪伪狂犬病病毒与猪圆环病毒2型的检测,并对检测结果进行了统计分析,了解滨州及其周边地区上述4种疫病的流行状况。     

Feasibility analysis of NIR for detecting sweet corn seeds vigor

This paper explores the feasibility of particle-based detection and grading of seed vigor based on a self-built seed single-granulation device using near infrared spectroscopy (NIRS). Sweet corn with uniform kernel size was used for this study. The seed samples were divided into three types, they were normal seeds, artificially aged seeds and heat-damaged seeds. A 2-part spectral acquisition of each seed were performed, one for the collection of seeds that fall into the detection zone within the separation pipe, another was on the static platform, whose collection was performed on 5 faces of each seed. Partial least squares discriminant analysis (PLS-DA) was used to classify the original data of the seeds. In the 2 parts, the discriminant results of the unprocessed normal seeds and the artificial accelerated aging seeds, the untreated normal seeds and the heat-damaged seeds showed that classification accuracy was higher than 98%. The research indicates that the spectral data of different positions of seeds can reflect their activity information, and it is feasible to detect and classify seeds in real time in the detection area of the separation pipeline.     

粉条加工中添加石蜡的检测技术研究

【目的】探讨粉条加工过程中非法添加石蜡的测定方法,为食品监管提供保障。【方法】先以石蜡成品摸索石蜡测定的气相色谱质谱条件,再以石蜡成分C18H38和C24H50进行回收率和相对标准偏差试验。【结果】不同预处理方法中,超声萃取处理的C18H38和C24H50在粉条中的萃取回收率最高,分别为93.5%和91.0%;20min超声时间萃取粉条中烷烃成分较为适宜;所建立的粉条中石蜡含量测定方法的相对标准偏差为3.6～4.8,样品回收率均大于90.0%,达到定量检测要求。【结论】所建立的石蜡检测方法操作简单,测定结果准确可靠,可用于粉条中石蜡成分的定性及定量测定。