Evaluation of two commercial PRRSV antibody ELISA kits with samples of known
status and singleton reactors |
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Authors: | Byoung-Joo SEO Hyunil KIM Ho-Seong CHO Byoung-Yong PARK Won-Il KIM |
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Institution: | 1)College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Iksan, Korea;2)Optipharm Inc., Osong, Korea |
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Abstract: | Two commercial PRRSV ELISA kits (IDEXX and Bionote) were evaluated for their sensitivity
and specificity using 476 PRRS-positive serum samples collected from 7 animal challenge
experiments and 1,000 PRRS-negative sera. Both ELISA kits exhibited 100% sensitivity with
sera collected 14 to 42 days post-infection, and the results from the kits were highly
correlated (R2=0.9207). The specificity of IDEXX or Bionote kit was 99.9% or
99.7%, respectively. In addition, the Bionote ELISA kit was used to examine 100 sera that
were determined to be falsely positive either by IDEXX 2XR or 3XR ELISA, and only 7 of
these samples were found to be positive. These results indicate that both ELISA kits
exhibited similar levels of sensitivity and specificity and would complement one another
for the verification of false-positive samples. |
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Keywords: | antibodies detection Bionote ELISA IDEXX ELISA nucleocapsid protein PRRSV |
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