Stable isotope variations in otoliths of Pacific halibut (Hippoglossus stenolepis) and indications of the possible 1990 regime shift

Stable oxygen and carbon isotope ratios (δ¹⁸O and δ¹³C) from archived otoliths of Pacific halibut, Hippoglossus stenolepis, were measured to examine the most recent regime shifts in British Columbia and the Gulf of Alaska. δ¹⁸O values of these otoliths ranged from −1.5 to +2.8‰ VPDB, and were consistent with the life stages and migration behavior of halibut. δ¹³C varied from −3.3 to +0.9‰ VPDB, but did not show a transition from the juvenile to the adult stage as does δ¹⁸O. Evaluation of δ¹⁸O and δ¹³C values of mature halibut (ages 8–12) indicated that the 1977 regime shift might have a warming impact on the northeast Pacific fish stocks. In contrast, a possible regime shift around 1990 with a bottom seawater temperature decrease of about 2 °C might have occurred in both the areas. The connection between stable isotope variations in otoliths and the climate regime shifts is thus potentially useful in studying the population dynamics of Pacific halibut, and decadal scale (e.g., the last 20–30 years) ocean environmental changes.     

The cytochrome P450 system of Atlantic salmon (Salmo salar): II. Variations in hepatic catalytic activities and isozyme patterns during an annual reproductive cycle

A group of Atlantic salmon (Salmo salar) was followed through their first year of maturation and spawning. At monthly intervals, starting with juvenile fish in December, 5–7 fish of each sex were killed, and liver and plasma were sampled. The last sampling point was of spawning fish in November a year later. Variables in the cytochrome P450 (P450) system were studied in hepatic microsomes, and estradiol 17 was measured in the plasma of females to assess the maturational status. The P450 1A1-mediated 7-ethoxyresorufin O-deethylase (EROD) started at high levels in winter, but decreased to non-detectable activities in pre-spawning females. Decreases, but not to the same extent, were also observed during this period in total cytochrome P450, cytochrome b₅, NADPH-cytochrome P450 reductase, and in the content of two immunochemically determined P450 isozymes. At the same time, LSI levels increased in maturing females (starting in July), and GSI levels increased in both sexes (starting in May). Sex specific differences were observed in pre-spawning fish in September and October, with levels of total P450, b₅, NADPH-cytochrome P450 reductase, EROD and P450 isozymes significantly lower in females. At the same time, plasma estradiol-17 levels reached peak values in females. The results point to the important role of sex steroids such as estradiol-17 as major factors in the regulation of final sexual maturation. However, this study also indicates that there may be estradiol-17 independent events of equal importance in the early stages of gonadal maturation that may involve the P450 system. The changes observed in the P450 system (as a major drug and steroid metabolizing system) of Atlantic salmon during sexual maturation may be of importance both in the endogenous transduction of hormonal signals, and as a pharmacological basis for designing therapeutic treatment of diseases in the aquaculture industry.Parts of this work were presented at the 5th International Symposium on Responses of Marine Organisms to Pollutants, April 1989 in Plymouth, United Kingdom (Larsen and Goks\/oyr 1989).     

Effects of dietary lipids on body composition and liver function in juvenile red drum, Sciaenops ocellatus

This study was conducted to evaluate the effects of different concentrations of dietary lipids on body composition and liver function in juvenile red drum, Sciaenops ocellatus. Diets were formulated to contain 40% crude protein from solvent-extracted menhaden fish meal and 0, 7, 14 or 21% lipid from menhaden fish oil. The basal diet, without supplemental fish oil, contained lipid at 0.4% of dry weight. The diets were fed to groups of 25 juvenile red drum initially averaging 7.3 ± 0.18 g fish^–1 in a recirculating culture system for 8 weeks and weight gain was recorded. After an additional 8 weeks, 16 fish from each treatment were sacrificed and the following measurements were recorded: hepatosomatic index (HSI), intraperitoneal fat (IPF) ratio, and liver -tocopherol, malondialdehyde (MDA) formation, and cytochrome P-4501A activity (measured as 7-ethoxyresorufin O-deethylase (EROD) activity). The activity of alanine and aspartate aminotransferases and concentrations of -tocopherol also were measured in plasma.Weight gain was significantly (p<0.05) affected by dietary lipid concentration, with values ranging from 361% of initial weight for fish fed the basal diet to 527% of initial weight for fish fed the diet containing 7% lipid. The HSI and IPF ratio values also were significantly affected by lipid with the lowest values recorded for fish fed the basal diet and the highest values observed in fish fed the diet containing 21% lipid. Increasing dietary lipid significantly increased oxidative stress as reflected in reduced -tocopherol in liver and plasma and increased MDA formation in the liver, although no overt pathological signs were observed. These findings suggest that lipid concentrations between 7 and 14%, when the diet contains 60 IU vitamin E kg^–1, are likely to limit oxidative stress and result in normal physiological responses of red drum.     

利用肠道菌群的分布和DNA序列对鲤科鱼类系统演化关系的研究

用检测到的肠道微生物菌群的含量对鲤科鱼类的草鱼（Ctenopharyngodon idellus)和青鱼（Mylopharyngodon piceus)、鲢（Hypophthalmichthys molitrix)和鳙（Aristichthys nobilis)、鲤（Cyprinus carpiohaematopterus)和鲫（Carassius auratus)，团头鲂（Megalobrama amblycephala)和三角鲂（Megalopbrama terminalis）8个种进行系统演化分析。所获得的系统演化树中，每个亚科内的物种邻接为1个接点。在亚科间，雅罗鱼亚科与鳇亚科相邻领接1个共同接点。该接点与鲢亚科和鲤亚科连结为1个共同祖选接点。用线粒体基因中的cytb和ND4基因全序列分别构建5个亚科13和11个种的NJ树，这2个树在5个亚科之间的邻接拓扑结构上亦不相同。3种树可推出一个共同的结论是在所分析的亚科中鲤亚科为最原始的类群，而鲫是最原始的类型。     

Distribution and induction of cytochrome P450 1A1 in the rainbow trout brain

Cytochrome P450 (CYP) 1A1 participates in the activation as well as detoxification of environmental pollutants such as aromatic hydrocarbons. This CYP form is also efficiently induced by aromatic hydrocarbons. The presence of CYP 1A1 in the brain might thus be of physiological and toxicological importance. In the present investigation on rainbow trout, the distribution of 7-ethoxyresorufin-O-deethylase (EROD) activity, a cytochrome CYP 1A1 catalyzed reaction, was measured in whole tissue homogenates from brain parts. In control fish, a relatively high activity was found in the rainbow trout olfactory bulb compared to the other brain parts. Although an EROD induction (3 to 7-fold) by β-naphthoflavone (BNF) was recorded in all brain parts from the rainbow trout, the highest induced activity was measured in the olfactory bulbs. To ascertain the distribution of EROD activity in cells, whole brain tissue was subfractionated by differential centrifugation. The fractionation scheme separated mitochondria (P2 fraction) and microsomes (P3 fraction) as determined by marker enzymes and electron microscopy. In control rainbow trout, a low EROD activity could be measured in the P2 fraction. BNF induced the EROD activity in both P2 and P3 fractions. Western blotting showed the induction by BNF of a protein band in the P2 and P3 fractions with a molecular mass around 58,000 when highly specific anti-cod CYP 1A1 antibodies were used. ELISA measurements confirmed the induction of CYP 1A1 protein in the rainbow trout brain subcellular fractions.     

Circulating levels and in vitro production of two maturation-inducing hormones in teleost: 17α,20β-dihydroxy-4-pregnen-3-one and 17α,20β,21-trihydroxy-4-pregnen-3-one, in a daily spawning wrasse, Pseudolabrus japonicus

The female bambooleaf wrasse, Pseudolabrus japonicus, spawns daily during the spawning season, and exhibits a diurnal rhythm of ovarian development. In the present study, we have investigated: (1) circulating levels of 17a, 20-dihydroxy-4-pregnen- 17,20-P) and 17,20,21-trihydroxy-4-pregnen-3-one (20-S) in females sampled at different times of the day during spawning season in captivity, and (2) in vitro production of 17,20-P and 20-S by follicle-enclosed oocytes at seven different develo tal stages. In addition, we developed a microtiter plate enzyme-linked immunosorbent assay (ELISA) for 17,20-P. Serum levels of 17,20-P and 20-S showed similar diurnal changes; substantial increases in these levels occurred around the time of germinal vesicle breakdown (GVBD). In vitro experiments showed that massive production of 17,20-P and 20-S occurred in follicles collected just before or during GVBD. Further, acute decreases in 17,20-P and 20-S production were found in the ovarian follicles just prior to ovulation, suggesting inactivation of the maturation-inducing hormone (MIH). These results, taken together with our previous data on the occurrence of GVBD in vitro, suggest a role for both 17,20-P and 20b-S as MIHs in the bambooleaf wrasse.     

Dietary Fucoxanthin Increases Metabolic Rate and Upregulated mRNA Expressions of the PGC-1alpha Network,Mitochondrial Biogenesis and Fusion Genes in White Adipose Tissues of Mice

The mechanism for how fucoxanthin (FX) suppressed adipose accumulation is unclear. We aim to investigate the effects of FX on metabolic rate and expressions of genes related to thermogenesis, mitochondria biogenesis and homeostasis. Using a 2 × 2 factorial design, four groups of mice were respectively fed a high sucrose (50% sucrose) or a high-fat diet (23% butter + 7% soybean oil) supplemented with or without 0.2% FX. FX significantly increased oxygen consumption and carbon dioxide production and reduced white adipose tissue (WAT) mass. The mRNA expressions of peroxisome proliferator-activated receptor (PPAR) γ coactivator-1α (PGC-1α), cell death-inducing DFFA-like effecter a (CIDEA), PPARα, PPARγ, estrogen-related receptor α (ERRα), β3-adrenergic receptor (β3-AR) and deiodinase 2 (Dio2) were significantly upregulated in inguinal WAT (iWAT) and epididymal WAT (eWAT) by FX. Mitochondrial biogenic genes, nuclear respiratory factor 1 (NRF1) and NRF2, were increased in eWAT by FX. Noticeably, FX upregulated genes of mitochondrial fusion, mitofusin 1 (Mfn1), Mfn2 and optic atrophy 1 (OPA1), but not mitochondrial fission, Fission 1, in both iWAT and eWAT. In conclusion, dietary FX enhanced the metabolic rate and lowered adipose mass irrespective of the diet. These were associated with upregulated genes of the PGC-1α network and mitochondrial fusion in eWAT and iWAT.     

铁皮石斛枫斗加工前后差异成分比较

铁皮石斛(Dendrobium officinale Kimura et Migo)的传统功效为益胃生津、滋阴清热,在降血糖方面具有较好的作用。前期研究发现,与铁皮石斛鲜条相比,枫斗的α-葡萄糖苷酶抑制率更高,且枫斗中化学成分种类丰富。为明确铁皮石斛枫斗与鲜条的差异性成分,阐明铁皮石斛加工成枫斗的科学内涵,本研究利用超高效液相色谱-四级杆串联飞行时间质谱仪(UPLC-QTOF-MS)在正离子模式下采集二者的代谢物信息,通过主成分分析、偏最小二乘判别分析等算法挖掘差异性化合物,并利用质谱碎片、数据库比对等方式鉴定差异性成分,最后采用聚类分析方法研究差异性成分在铁皮石斛枫斗和鲜条中的含量变化规律。经过筛选和鉴定共得到16种差异性化合物,其中柚皮素、4,4'-二羟基-3,5-二甲基联苄、诺比林A、石斛酚等12种黄酮、联苄类化合物在枫斗中含量较高,而α-亚麻酸、L-苯丙氨酸、草质素等4种脂肪酸、氨基酸类化合物在鲜条中含量较高,这些差异性成分可能是潜在的铁皮石斛枫斗降血糖功能成分。本研究结果为阐明铁皮石斛枫斗降血糖物质基础提供了数据支撑,对进一步促进铁皮石斛精深加工具有重要意义。     

长江和闽江长体鳜遗传多样性的 细胞色素b全序列分析

对长江水系中江西都昌和闽江水系中福建建瓯2 个群体23 尾长体鳜细胞色素b 全基因的1 141 bp 序 列进行分析,发现15 个变异位点,其中简约信息位点7 个,共有13 个单倍型,总体单倍型多样度（Hd）和核苷酸多态 度（Pi）分别为0.933（依0.030）和0.00241（依0.030）,呈现出高单倍型多样性和低核苷酸多样性的特点。中性检验结果显 示Fu爷s Fs为显著负值,核苷酸不配对分析呈现单峰分布,表明长体鳜在历史上经历过种群扩张事件,推测扩张年代 约为6 万年前,为更新世晚期。在邻接树和简约性网络图中不同地理来源的单倍型交错分布,群体间的Fst 和Nm 值 分别为0.06667 和3.5。AMOVA 分析表明,长体鳜群体内遗传差异（95.17%）大于群体间（4.83%）遗传差异,遗传变异 主要是集中在群体内部,表明都昌和建瓯的长体鳜群体间没有出现明显遗传分化,可作为一个管理保护单位。