Adjuvant immunotherapy of feline fibrosarcoma with recombinant feline interferon-omega

BACKGROUND: Recombinant feline interferon-omega (rFeIFN-omega) was tested as a treatment option for cats with fibrosarcoma to assess safety and feasibility. HYPOTHESIS: Treatment with rFeIFN-omega in cats with fibrosarcoma is safe and feasible. ANIMALS: Twenty domestic cats. METHODS: In an open-labeled uncontrolled clinical trial 12 injections of 1 x 10(6) U/kg rFeIFN-omega were administered over a 5-week period: the 1st through 4th injections were given intratumorally, and the 5th through 12th injections were administered subcutaneously at the tumor excision site. Wide surgical excision of the tumors was carried out after the 4th injection and before the 5th injection of rFeIFN-omega. A Common Terminology Criteria for Adverse Events (CTCAE) analysis was conducted. Flow cytometry of fibrosarcoma cells after incubation with rFeIFN-omega and recombinant feline interferon-gamma was performed to assess the biological effect of rFeIFN-omega. RESULTS: Changes in blood cell count, increases in serum aspartate-amino-transferase activity, serum bilirubin concentration, serum creatinine and serum electrolyte concentrations, weight loss, anorexia, increased body temperature, and reduced general condition were observed but were mostly minor (grade 1 and 2) and self limiting. Eosinophilia (P = .025), neutropenia (P = .021), and weight loss (P < .001) were statistically correlated with rFeIFN-omega-treatment (analysis of parameters before treatment and after 3 injections of rFeIFN-omega). Flow cytometry of 5 unrelated feline fibrosarcoma cell lines showed increased expression of major histocompatibility complex (MHC) class I molecules (P = .026) in response to in vitro incubation with rFeIFN-omega, whereas expression of MHC class II molecules was not affected significantly. CONCLUSIONS AND CLINICAL IMPORTANCE: RFeIFN-omega for the treatment of feline fibrosarcoma is safe, well tolerated, and can be easily performed in practice. To assess the efficacy of the treatment, it should be tested in a placebo-controlled trial.     

Pre-partum monensin supplementation improves body reserves at calving and milk yield in Holstein cows dried-off with low body condition score

The objective of this study was to determine the effect of a monensin controlled-release capsule administered intraruminally at drying-off on body condition score (BCS) at calving, milk yield, fertility and concentration of energy-related blood metabolites in Holstein cows dried-off with low BCS (< or = 3.0, scale 1 to 5 with a 0.25 point of increment). Between July and August, 2001, 220 cows from parity 2 or more and dried-off 50-70 days before expected parturition, with a BCS < or = 3.0 were randomly assigned to either a treatment group (n=110; oral capsule of monensin releasing 335 mg/day for 95 days) or a control group (no capsule, n=110). At assignment, on day 21 before expected parturition, at calving, and at 7, 14, and 21 days in milk a blood sample was obtained from a random sub sample of 10 cows per group. Effects of monensin on serum NEFA, BHBA and glucose were measured. Milk yield, milk fat and protein content (%) at DHIA test days during the entire lactation, 305 ME milk production and reproductive responses were compared. Monensin significantly improved BCS at calving, increased milk yield at test days 4 and 8, decreased the percentage of milk protein, did not change the percentage of milk fat, and decreased NEFA and BHBA during the post-partum period.     

除草剂莠去津降解菌SFAD3的分离鉴定及其土壤修复潜力

为获得能够修复除草剂莠去津污染土壤的高效降解菌,采用摇瓶富集法、平板分离法从莠去津过量使用的土壤中分离得到降解菌SFAD3,通过形态学、生理生化特征观察以及16S rDNA和ITS序列分析进行种类鉴定,测定获得菌株的最适降解条件,并通过土壤接种和盆栽试验验证菌株对莠去津污染土壤的修复作用。结果表明,菌株SFAD3最终被鉴定为门多萨假单胞菌Pseudomonas mendocina,该菌株培养30 d时对污染土壤中50 mg/kg莠去津的降解率可达72.6%;菌株SFAD3在MM液体培养基中最适降解条件为温度37℃、pH 7、莠去津初始浓度6.25 mg/L、接种量2%,对莠去津降的降解率为50.0%～72.2%;与仅有莠去津的处理相比,添加有SFAD3发酵液的处理20 d后芝麻的株高、根长、湿重和干重能够显著恢复,并且该菌对芝麻还具有一定的促生作用。表明降解菌SFAD3在修复莠去津污染土壤方面具有潜在的应用价值。     

Effect of the Silicic Acid Polymerization Degree on the Coagulating Property of Polyalumium Silicate Chloride

Polyaluminum silicate is a new type of inorganic macromolecular flocculants, which is developed as a complex product in recent years on the base of polyalumium flocculant and polysilicic acid flocculant. Sodium silicate is diluted to the concentration of 3.0% SiO 2 with distilled water. The solution was adjusted to pH 4.5 with sulphate acid and kept at quiescency for different time to get polysilicic acid with different polymerization degrees. Using polysilicic acid prepared and aluminium chloride as materials, a series of polyaluminium silicate chlorides(PASC) with different basicities(B), Al/Si molar ratios and polymerization degree of silicic acid are prepared by copolymerization technique. The Al-Ferron timed complex colorimetric method is applied to characterize species distribution of PASC and to study its influence factors. The interaction between hydrolyzed aluminum species and polysilicic acid with different polymerization degrees in PASC is discussed.The flocculant prepared is applied to the treatment of commercial concrete wastewater. The turbidity removal of commercial concrete wastewater is up to 97% when a PASC, prepared with polysilicic acid of 145 min polymerization degree.     

DYNAMIC ANALYSIS OF SPINDLE

In this paper, taking a D1201 spindle as an example, we discuss the dynamic analysis of spindle by using the theory of the complex model, we also analyse the factors which effect the dynamic characterics of spindle and give results which arc reference value to improving the structure of spindle,     

Optimization of Energy Dissipation Braces in Frame Structures

In this paper, the complex method of the nonlinear programming is used to optimize the parameters of energy dissipation bracing in energy dissipation braced frame. This method is realized with computer program and proved to be efficient. A 6-story reinforced concrete frame is used as a demonstrative example.     

Asymmetric Exhaustive Service Polling System with Bernoulli Feedback

This paper studies asymmetric exhaustive service polling system with Bernoulli feedback,gives the distributions of queue lengths and sojoun times,and obtains some other results,in stationary condition.     

ANALYSIS OF OPERATING CONDITIONS FOR DIAGONAL MINE FANS

We shall discuss the real opererating conditions of the mine fans . in the mine diagonal ventilation system and establish a set of mathematics model for analysing the real engineering to provide judgement for technical manage ment of mine ventilation.     

直墙拱形巷道围岩应力场分析

为得出直墙拱形巷道围岩应力分布规律,应用复变函数弹性理论推导了直墙拱形巷道围岩应力分布的解析表达式。对直墙拱形巷道边界的围岩应力和巷道水平线方向的围岩应力分布规律进行分析,并考虑直墙拱形巷道断面高宽比和侧压系数对其影响规律。研究表明：在不同巷道断面高宽比、侧压系数下,直墙拱形巷道围岩应力集中区域主要集中在直墙底部底角处、拱形顶板中点附近和底板中部3个位置。不同巷道断面高宽比下,直墙拱形巷道沿水平线的应力分布规律基本相同。侧压系数大于1时,采用巷道断面高宽比小于1较有利于巷道稳定;侧压系数小于等于1时,采用巷道断面高宽比大于1较有利于巷道稳定。