苏云金杆菌HBF-1菌株在土壤中毒蛋白含量的ELISA检测

以苏云金芽孢杆菌HBF-1菌株中分离纯化的高特异性杀虫蛋白作为抗原，免疫新西兰白兔制备出多克隆抗体，其效价可达到16000。应用制备的特异性抗体建立了间接酶联免疫吸附测定法（ELISA），检测不同土壤样品中Bt毒蛋白含量。初步结果表明，该方法能够检测土壤中Bt毒蛋白的含量，因土壤理化特性及组成成分的不同，检测到的毒蛋白含量也略有不同，河沙中的蛋白检测量最低，只有12．53％；另一种沙壤混合物中检测量达到76.16％。     

逆固相基质分散净化和气质联机检测土壤中的六氯苯残留

建立了逆固相基质分散净化和气质联机法(GC-MS)快速、简便测定土壤中六氯苯(HCB)残留量的方法。以丙酮-正己烷(1∶ 1,体积比)为提取剂,采用超声波萃取,经固相基质PSA(primary secondary amine)吸附净化后采用GC-MS选择离子模式检测。结果表明,六氯苯在0.05~5 mg/L浓度范围内呈现出较好的线性关系,相关系数R2=0.999 5; 方法检测限为0.3 μg/kg,定量限为0.9 μg/kg,在0.89、1.78、8.90 mg/kg 3个添加水平上的平均回收率分别为106.6％、108.2％和108.1％。用该方法测定了天津某生产厂厂区地下1~10 m深的土壤样本,发现六氯苯的残留量在2.3~25 912 mg/kg 之间,表现出显著的空间变异趋势。该方法快速、简便、经济、高效、实用并且安全,适合大批量样本中六氯苯的快速检测。     

豆瓣菜不同茎节扦插繁殖试验

以豆瓣菜葡匐茎的上,中、下三段的试材,进行不同茎段的扦插繁殖试验结果表明,中段成活率高,分枝快,适于做扦插材料。     

淹水、干旱条件下北空越桔生理反应的研究

采用盆栽试验,研究了'北空'越桔在连续淹水、干旱条件下的生理反应。结果表明,在淹水和干旱条件下,越桔叶片的光合强度、叶绿素含量（Chla、Chlb、Chla＋b、Chla／Chlb）、光量子通量密度降低,而呼吸强度、气孔导度、蒸腾速率升高,随逆境时间加长,变化幅度增大。     

Stem-base disease and fungal colonisation of winter wheat grown in compost inoculated with Fusarium culmorum, F. graminearum and Microdochium nivale

Fungal colonisation of winter wheat cv. Cadenza by Fusarium culmorum, F. graminearum and Microdochium nivale was studied under conditions designed to avoid the splash dispersal of conidia from infested compost, to evaluate the possibility that systemic growth may transfer infection from the stem-base to the head. At decimal growth stages 33, 59, 77–87 and 95 the extent of fungal growth was assessed using a sample of 72 plants, by the recovery of fungal species from the stem-base, from each node and from the ear. Each of the fungi was recovered from stem tissues above soil level in some, apparently symptomless, plants. Symptoms of Fusarium foot rot were seen in an increasing proportion of plants during grain-fill and desiccation. There was an inverse relationship between recovery and the height above stem-base from which the stem tissue was excised. F. culmorum was the most frequently isolated fungus and it was also recovered from the highest position in plants. Only 3% of plants were colonised above the second node and none of the fungal species were recovered from either the fifth node or the ear. This suggests that colonisation and systemic growth from Fusarium infested compost is unlikely to contribute to the development of ear blight symptoms in winter wheat.     

奎屯,乌苏,独山子地区水资源污染现状

通过对本区水质监测资料,废水排放资料的分析,评价区域水资源污染情况,找出目前主要的水环境问题,提出解决区水环境问题的途径或措施。     

毛乌素沙地中几种植物水分特性的研究

用ＬＩ－１６００稳态气孔计研究了我国干旱半干旱地区毛乌素沙地恢复重建生态系统中几种主要植物的蒸腾作用及产量比较。结果表明：羊柴平荐处理后,生长旺盛,蒸腾速率加快,提高;日本大粒莉麦可利用较深层地下水分,维持旺盛的生长活动,     

陆生固氮蓝藻对土壤环境的影响

我们于１９８８－１９９０－年通过野外调查和室外试验,探讨了陆生固氮蓝藻对土壤环境的影响。试验证明,陆生固氮蓝藻可以利用空气中的分子态氮合成氮素化合物,不断地加富土壤中的氮化物。同时,由于它们大量的繁殖和死亡腐解也增加了土壤中的有机物质使土肿微生物的生长和活动更加旺盛,土     

坡长对径流及侵蚀的影响

张家口市水保试验站坡长小区五年观测资料表明,降雨强度影响径流量、侵蚀量随坡长的变化。降雨强度较小时,径流量不能用坡长与降雨强度的乘积代替,侵蚀量随坡长增加较慢。降雨强度较大时,径流量可用坡长与降雨强度的乘积代替,侵蚀量方程中坡长指数较大。     

柴达木盆地水资源与合理开发利用研究

本文通过对柴达木盆地水资源量、农业水资源开发利用现状分析,结合盆地水土资源匹配情况与经济发展方向,提出了实现盆地水资源合理开发利用的基本途径,并指出在实施水资源开发利用中所要注意的具体问题,为盆地可持续发展提供依据。