槲皮素对PEDV感染幼龄仔猪的生长性能、血液生化指标和肠道吸收与屏障功能的影响

【目的】 试验旨在探讨槲皮素在防治仔猪感染猪流行性腹泻病毒（PEDV）中的作用。【方法】 选取18头体重相近、健康的7日龄仔猪（杜×长×大）,随机分为3组（对照组、PEDV组和PEDV+槲皮素组）,每组6个重复,每个重复1头猪,试验期共11 d。经过3 d适应期后,于试验第4~10天给PEDV+槲皮素组仔猪口腔灌服10 mg/kg BW的槲皮素,其他组仔猪口腔灌服等体积的人工乳,于试验第8天给PEDV组与PEDV+槲皮素组仔猪口腔灌服1×10^4.5TCID₅₀的PEDV,对照组灌服等体积的PBS溶液。试验第11天早上空腹称重,全部仔猪灌服D-木糖（0.1 g/kg BW）,1 h后经前腔静脉采血,检测血液生化指标、血浆二胺氧化酶（DAO）活性和D-木糖含量;将所有仔猪屠宰取空肠黏膜,检测肠道屏障相关基因,包括肠绒毛蛋白（villin）,紧密连接蛋白-1（claudin-1）,闭合蛋白（occludin）和肠型脂肪酸结合蛋白（iFABP）的相对表达量。【结果】 与对照组相比,PEDV组仔猪平均日增重显著下降（P<0.05）,粪便评分显著升高（P<0.05）;血液中高密度脂蛋白含量显著降低（P<0.05）,肌酐和尿素氮的含量显著提高（P<0.05）;血浆中D-木糖含量显著降低（P<0.05）;空肠claudin-1、iFABP基因的相对表达量显著下调（P<0.05）。与PEDV组相比,PEDV+槲皮素组仔猪平均日增重和血浆D-木糖含量显著升高（P<0.05）;血液中肌酐和尿素氮的含量显著降低（P<0.05）;空肠claudin-1、villin和iFABP基因的相对表达量显著上调（P<0.05）。【结论】 10 mg/kg BW槲皮素可在一定程度上缓解PEDV感染导致的仔猪生长抑制和肾功能损伤,增强肠道吸收与屏障功能。     

广东康禾自然保护区植物区系研究

根据对保护区的植被调查,分析了该保护区的植物区系、生活型谱和植被类型,结果显示该保护区植物区系组成以泛热带和热带亚洲成分最为丰富,各占30.5%和13.8%;生活型谱以高位芽植物占绝对优势,但比例略低于近纬度带的阴那山等地区;植被类型主要为亚热带常绿阔叶林,但因人为破坏较严重,现有植被主要为次生性较强的常绿阔叶林和针阔叶混交林。     

山地植物群落生物多样性与生物生产力海拔梯度变化研究进展

文中从生物学谱中群落和生态系统尺度，根据国内外的野外观测文献，总结了山地植被在群落生物多样性和生物生产力2方面的海拔梯度变化特征，以及群落生物生产力与生物多样性的关系，并提出目前该领域研究的几个热点问题。     

红树植物地面反射光谱特征研究

结合使用野外便携式光谱仪对九龙江口红树林保护区白骨壤、秋茄、桐花树3种红树植物的叶冠表观光谱的测量结果,系统分析比较了红树植物叶冠反射光谱特征及其差异;并在一个潮间带底质遥感信息处理平台上对研究地点进行基于表观光谱特征的白骨壤和秋茄的SPOT卫星遥感图像分类;最后讨论了红树光谱研究中存在的问题。     

竹炭吸湿性的初步研究

选用箱式电阻炉和土窑烧制的竹炭,在不同温度和湿度条件下进行竹炭吸湿性变化的试验.结果表明:(1)500℃、600℃、800℃和土窑(二)的竹炭其吸湿性与温湿度有较显著关系;而700℃竹炭和土窑(一)竹炭的吸湿性与温湿度之间没有显著关系.在一定温湿度范围内,湿度对竹炭吸湿性的影响比温度显著.(2)不同炭化温度之间的竹炭其吸湿性不同.800℃的竹炭其吸湿性最大,其值为11.5%,500℃的竹炭最小,其值为8.6%;600℃的竹炭8.8%;700℃的竹炭为11.3%.(3)不同土窑的竹炭其吸湿性有显著差异.土窑(一)和土窑(二)的竹炭吸湿值分别为9.8%和6.4%.     

三羟鹅膏毒肽的反相高效液相色谱分离纯化

用反相高效液相色谱法,以0.02mol醋酸铵-乙腈为流动相和梯度洗脱模式,从灰花纹鹅膏菌中分离纯化出三羟鹅膏毒肽(amanin),产品纯度达96%以上,三羟鹅膏毒肽的分子量为903.3.     

柠条锦鸡儿组培苗继代培养中矿质元素的吸收规律

比较柠条锦鸡儿在MS、WPM、B5三种基本培养基中对P、K、Ca、Mg、Mn、Cu等主要矿质元素的吸收规律,用向量分析方法找出三种基本培养基中的限制因子.结果表明:矿质养分整体供应水平是影响柠条锦鸡儿组培苗吸收矿质元素的关键因子;除此之外,MS培养基的Ca、P水平偏低,WPM和B5培养基的主要限制因子分别为K、Mn和Ca、P、K.     

麦秸/聚氨酯复合人造板的工艺对其吸声系数的影响研究

通过变化麦秸和聚氨酯的比例、麦秸/聚氨酯复合人造板的密度、以及发泡温度,研究了麦秸/聚氨酯复合人造板在不同的声波频率下的吸声系数。试验结果表明:在不同的声波频率下,发泡温度的高低对麦秸/聚氨酯复合人造板的吸声系数有不同的影响;麦秸/聚氨酯复合人造板的密度与其吸声系数的大小关系密切;在声波频率为250～4 000Hz区域内,麦秸和聚氨酯的比例对麦秸/聚氨酯复合人造板的吸声系数影响较大。     

Extractives relating to heartwood color changes in sugi (<Emphasis Type="Italic">Cryptomeria japonica</Emphasis>) by a combination of smoke-heating and UV radiation exposure

 Sugi green logs with red or black heartwood were smoke-heated, and the changes in the color of the heartwood after ultraviolet (UV) (λ = 365 nm) radiation exposure were then observed. After UV radiation exposure, the redness and yellowness increased in both the red and black heartwoods, whereas the brightness decreased. In the black heartwood, the resulting color turned from yellowish white to reddish brown. Reddening in black heartwood after exposure to a combination of smoke heating and UV radiation is thought to be due to a decrease in brightness and an increase in both redness and yellowness. However, the degree of change in heartwood color by UV radiation exposure was not greatly affected by smoke-heating treatments of various lengths. When methanol extracts were fractionated and exposed to UV radiation, the yellowness increased in the n-hexane-soluble portion and the redness increased in the acetone-soluble fractions from the n-hexane-insoluble portion. These results suggest that the n-hexane-soluble fraction contains the substances that allow heartwood color to change to yellow after UV radiation exposure, and the acetone-soluble-fraction from the n-hexane-insoluble portion contains the substances that allow it to change to red. Received: November 14, 2001 / Accepted: June 3, 2002 Acknowledgment This research was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science. This study was presented in part at the 51st Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001 Correspondence to:N. Yoshizawa     

Power spectrum density and experimental modal analysis of wide belt sander applied in domestic wood industry

This paper took the upper-lower wide belt sander B229 with four-feet wide belts, manufactured in China, as the study target. By means of framework dynamic design, we study its vibration characteristics by commencing from the place having horizontal defects and used experimental modal analysis (EMA) and power spectrum density (PSD) to observe the sanding parts and the whole machine, respectively. In the modal test, we mainly adopted the cross spots testing method to get the frequency response function of the fixed spots to every excitation vibration spot, then applied the SISO frequency response function and the frequency response function fitting method to identify and complete parameter recognition, respectively. The typical frequency response function chart of the whole machine and its sanding parts, as well as its second-order mode charts of contacting roller, were obtained. Through PSD analysis, we can get the amplitude-frequency spectrum and drive frequency. Translated from Scientia Silvae Sinicae, 2005, 41(6): 132–137 [译自: 林业科学]