GA_3处理对天女木兰种子解除休眠过程中碳水化合物含量的影响

研究了GA3对天女木兰种子在变温层积条件下解除休眠过程中碳水化合物的影响,以此探讨外源赤霉素(GA3)对层积过程中天女木兰种子解除休眠的作用机理。结果表明,1000mg/LGA3水溶液处理极显著促进天女木兰种子萌发,在整个层积过程中,GA3水溶液处理诱导淀粉含量下降;蔗糖含量变化剧烈,在层积90d降至最低,果糖和葡萄糖含量显著增加;在层积后期(90～120d)果糖和葡萄糖含量下降,蔗糖含量上升,从而说明了碳水化合物参与并调节了天女木兰种子休眠解除这一过程。     

中华芦荟的离体快繁技术研究

[目的]探索芦荟的离体快繁技术,为芦荟的组织培养提供试验依据。[方法]以盆栽中华芦荟的嫩茎尖为外植体,MS为基本培养基,不同的增殖阶段添加不同的植物激素进行离体快繁试验。[结果]培养30 d后,3瓶被污染,4瓶不长愈伤组织,13瓶在芽基部产生圆球状浅黄色的愈伤组织。在诱导芽分化约20 d后,2瓶被污染,11瓶开始芽点分化。3.00 mg/L 6-BA的处理效果最佳。诱导生根11 d后,1瓶被污染,2瓶出现叶子干焦的现象,17瓶长势良好。中华芦荟愈伤组织诱导的适宜培养基为MS＋6-BA 2.50 mg/L＋NAA0.15 mg/L,诱导率为60%;丛生芽分化及继代的适宜培养基为MS＋6-BA 3.00 mg/L＋NAA 0.10 mg/L,诱导率为85%;生根的适宜培养基为MS＋6-BA 0.30～0.50 mg/L＋IBA 0.20 mg/L＋活性炭0.5%。[结论]该研究为芦荟的快繁提供了初步的理论依据。     

铁路货车电能收集装置的机械结构设计

针对转K6型转向架,设计了一种适用于铁路货车的低成本、利用自身能量发电,满足铁路货车低功率用电需求的供电装置。此装置利用滚珠丝杠将悬挂系统的上下振动转变为发电机转子的旋转。通过分析弹簧中的空间以及货车运行过程中的振动情况,设计了滚珠丝杠、外壳等零件,并通过SolidWorks软件对装置进行了仿真。     

Soil Properties Degradation Assessment Using Infrared Scanning Technique of Soils （Case Study： Eastern of Congo）

Recently, near infrared reflectance （NIR） and mid-infrared （MIR） spectroscopy techniques are increasingly introduced as convenient and simple non-destructive techniques for quantifying several soil properties. This study uses MIR method to predict pH, soil organic C, total N, AI, Ca, Mg and K, CEC and soil texture for soil samples collected in Sud-Kivu, Congo. A total of 536 composite soil samples were taken from two locations （Burhale and Luhihi） at two depths （0-20 cm and 20-40 cm） using a spatially-stratified random sampling design within an area of 200 km2. Differences in characteristics were evaluated between the two locations, land use （cultivated vs. non-cultivated land） with soil depths. A random subset of the samples （10%） were analyzed using standard wet chemistry methods, and calibration models developed by MIR data to estimate soil properties for the full soil sample set. Partial least squares regression （PLS） method gave acceptable coefficients of determination between 0.71 and 0.93 for all parameters. Soil organic matter levels were higher in cultivated plots in Luhihi （3.9% C） than in Burhale （3.0% C）, suggesting lower levels of soil fertility in the later area. This indicates high levels of acidity, which are likely to limit crop production in the area. Phosphorus deficiency is acute in Burhale （2.4 mg P/kg） but less in Luhihi （5.4 mg P/kg）. In both locations, low levels of Ca and Mg indicate that soils may be susceptible to deficiencies in both elements.These findings provide new opportunities for monitoring soil quality in the region which can benefit multiple actors and scientists involved in the agricultural and environmental sectors.     

Effects of soil water availability on development of suberin lamellae in the endodermis and exodermis and on cortical cell wall thickening in red bayberry (Myrica rubra Sieb. et Zucc.) tree roots

The effects of soil water availability on suberin lamellae formation in the endodermis and exodermis and the occurrence of cell wall thickening in the cortex in red bayberry (Myrica rubra Sieb. et Zucc.) tree roots were examined during tissue aging. For several months, red bayberry trees were grown in small baskets under dry, normal, and waterlogged soil water conditions. Transverse sections of roots from 5 mm from the tip to the basal portion were stained with several staining solutions and the cell structure was observed. Root anatomical development was significantly changed by soil water conditions. The suberin lamellae in the endodermis formed later in plants grown under dry conditions than in those grown under waterlogged conditions. Cell wall thickening in the cortex near the endodermis was promoted by drought, but apparently not by waterlogged conditions.     

Cost-efficient light control for production of two campanula species

A cost-efficient light control system based on weather forecasts, electricity prices and daily photosynthesis integral (DPI) was evaluated for application in the commercial production of the long-day (LD) plant Campanula portenschlagiana ‘Blue Get Mee’ and C. cochlearifolia ‘Blue Wonder’. Experiments were conducted under both autumn and spring conditions and included four treatments. Three treatments were controlled by the software system DynaLight Desktop which automatically defined the most cost-efficient use of supplemental light, -based on a predefined set point of DPI, forecasted solar irradiance and the market price on electricity. The set points of DPI in the three treatments were 300, 450 and 600 mmol CO₂ m⁻² leaf d⁻¹ and the treatments were compared with a traditional LD 19-h treatment. The DPI-based light control strategy resulted in very irregular light patterns including daily periods of solar irradiance combined with supplemental light in low light periods and a night period interrupted by irregular light breaks (NB-lighting). Both campanula species flowered in the DPI-based treatments during spring, but the flowering percentage was low and non-uniform during autumn. This was caused by a combination of the irregular light, low natural light intensities and a decrease in daily light integral (DLI), and could be restored by maintaining a continuous 19 h photoperiod with incandescent lamps (<5 μmol m⁻² s⁻¹), illustrating that photoperiod was an important factor for flowering in LD species grown under low light intensities. Growth in terms of carbon gain was marginally affected by the irregular light and a 25% reduction in electricity costs was achieved without major reductions in plant quality in spring. Our results illustrate that plant production of LD species can be maintained in a cost-efficient light control system where the use of supplemental light is based on weather forecasts and electricity prices.     

重庆市万州区1年生尾巨桉生长情况调查

通过对重庆市万州区营造尾巨桉(3229品系)的调查结果表明,该树种在该区域的适应性和速生性较强,可进一步扩大种植范围,在万州区乃至三峡库区具有一定的发展前景。     

基于最小二乘法的落叶松木粉粒径数学建模与分析

【目的】建立落叶松木粉粒径与长宽比的数学模型，通过分析数学模型和其二阶导数，揭示木粉长宽比随粒径减小的变化趋势及变化的根本原因，获得最大长宽比对应的粒径，为建立长宽比与力学性能之间的定量关系提供参考。【方法】利用光学显微镜拍摄获得木粉的显微图像，测算获得目标木粉成熟管胞的平均长度、平均宽度以及木粉粒径的大小。通过数字图像处理技术提取单木粉颗粒的矩形度、长宽比：将原始木粉显微图像由 RGB颜色空间转到Lab颜色空间，提取其b分量；对b分量图像用3×3模板进行中值滤波；用K-means算法将去噪后图像聚类为2类，得木粉的二值图像；对二值图像用5×5的结构元素进行先开启后闭合的数学形态学运算；用八连通区域法标记图像中的单木粉颗粒；对标记后图像用目标区域像素点个数统计法计算获得单木粉颗粒的几何面积，用主轴法获得单木粉颗粒的最小外接矩形的长、宽、面积；计算获得单木粉颗粒的长宽比、矩形度数据。采用最小二乘法对木粉粒径与长宽比进行数据拟合，通过分析评判多项式、高斯和傅里叶3种拟合函数后选用高斯方程表达得木粉粒径与木粉长宽比的数学模型，再根据其拟合曲线方程计算得其二阶导数，结合模型的二阶导数和测算得的木粉管胞数据对高斯模型进行分析与讨论。【结果】矩形度不随木粉粒径的减小而变化，均值在0.6～0.8之间。长宽比随粒径减小出现先增大后减小的趋势：木粉粒径在1100～576μm 时，长宽比数值从接近于1开始逐渐增大；木粉粒径为576μm时(与本文目标木粉成熟管胞的平均长度563.82μm接近)，长宽比达到最大数值4.6；木粉粒径在576～30μm时，长宽比逐渐减小；而粒径小于50μm 时(与目标木粉成熟管胞的平均宽度46.498μm接近)，长宽比数值再次趋近于1。【结论】长宽比的变化与管胞破裂密切相关：木粉粒径大于管胞长度时，木粉主要通过纵向断裂使粒径减小；粒径与管胞长度接近时，长宽比较大；粒径小于等于管胞宽度范围内，木粉主要是横向断裂，而长宽比基本不再发生变化且趋近于1。长宽比和冲击强度随粒径减小的变化趋势是一致的，长宽比是影响材料力学性能本质因素之一。     

小花忍冬硬枝扦插影响因素分析

小花忍冬是观赏性和抗逆性兼具的优良树种之一。为了解决其种子繁殖子代分化变异大的问题,结合小花忍冬特性,通过不同基质、激素及不同浓度的处理,对其进行了硬枝扦插试验。结果表明：基质种类、激素种类、激素浓度都直接影响其扦插成活率,激素种类直接影响地下根系的生长量。其中,使用珍珠岩︰沙子=1︰2、NAA、100 mg/L组合,...