抗鸡CD25单克隆抗体的制备与鉴定

用纯化的鸡IL-2Rα(chCD25)重组蛋白免疫BALB/c小鼠,取其脾细胞与SP2/0骨髓瘤细胞融合,对杂交瘤细胞及时筛选,阳性孔经3次有限稀释法克隆,成功获得1E1,1G1,4H5,5E1,6A1,6C2,6C9 7株能稳定传代并分泌抗chCD25单克隆抗体的杂交瘤细胞.该7株单克隆抗体的腹水间接ELISA效价达106以上.除了1E1为IgG2b外,抗体类型均为IgG1,轻链皆为κ.经Western blot和免疫细胞化学分析表明,7株单克隆抗体皆与chCD25重组蛋白和真核表达蛋白EGFP-chCD25反应.抗chCD25单克隆抗体的获得为开展禽类分子免疫学研究提供了一种新的工具.     

甘蔗新品种桂糖25号的选育

桂糖25号(桂糖96-44),亲系:CP72-1210×崖城71-374,中茎,早熟、高糖、高产,宿根性好,抗逆性强,适应性较广。在国家甘蔗品种“863”区域试验中,产蔗量(95.5t/hm2)较对照(新台糖10号)增产17.2%,甘蔗蔗糖分(15.24%)较对照高1.00%,含糖量(14.5t/hm2)较对照增产26.1%。适应于广西、广东、云南、福建等蔗区种植。     

1α,25-二羟维生素D_3对大鼠成骨细胞增殖分化及周期的影响

在SD大鼠成骨细胞(Osteoblast,OB)体外培养体系中添加不同浓度1α,25-二羟维生素D_3(0、10~(-9)、10~(-8)、10~(-7) mol/L),作用24、48、72 h,测定OB增殖率、碱性磷酸酶(ALP)活性,作用48 h流式细胞仪测定OB周期.结果显示,10~(-9)mol/L 1α,25-二羟维生素D_3作用24、48、72 h均促进OB增殖(P<0.05或P<0.01),抑制ALP活性(P<0.01);10~(-8)、10~(-7)mol/L作用24、48 h,OB增殖率与对照组差异不显著(P>0.05),但24 h时ALP活性均明显升高(P<0.05或P<0.01),48 h则抑制了ALP活性并使OB滞留在G_2/M期(P<0.05或P<0.01);72 h时10~(-7) mol/L组OB增殖率极显著低于其余各组(P<0.01),并使ALP活性升高(P<0.01).表明低浓度1α,25-二羟维生素D_3能促进OB增殖,抑制其分化;高浓度1α,25-二羟维生素D_3能抑制OB增殖,促进其分化,并使细胞滞留在G_2/M期.     

25%嘧菌酯悬浮剂对黄瓜霜霉病的防效研究(英文)

[目的]研究25%嘧菌酯悬浮剂对黄瓜霜霉病的防效。[方法]试验设空白对照和25%嘧菌酯悬浮剂450、600、750 g/hm2以及80%代森锰锌可湿性粉剂300 g/hm2 5个处理,于2012、2013连续2年开展了25%嘧菌酯悬浮剂防治黄瓜霜霉病田间药效试验。[结果]25%嘧菌酯悬浮剂对黄瓜霜霉病有较高的防治效果,其防效为70.0%~83.6%,使用时推荐剂量为25%嘧菌酯悬浮剂600 g/hm2,于黄瓜霜霉病发病前或初开始喷药,间隔7 d喷一次药,共喷药3次。[结论]该研究可为25%嘧菌酯悬浮剂在生产上的推广应用提供理论依据。     

In vivo calcitropic effect of some vitamin D compounds in the marine Antarctic teleost,Pagothenia bernacchii

The Antarctic notothenioid, Pagothenia bernacchii, were found to have plasma total and free calcium levels, plasma inorganic phosphate and whole body calcium efflux rates which were similar to those seen in other teleosts. But total bone calcium was lower than reported for other teleosts. A single injection of vitamin D₃ (5 ng g^–1 fish) increased plasma total and plasma free calcium and these increases were associated with an increase in whole body calcium efflux and bone calcification. Conversely, the same treatment with 1,25-(OH)₂-D₃ reduced plasma free calcium. This seco-steroid also increased the specific activity of ⁴⁵Ca in bone at 40h post-injection but did not significantly effect total bone calcium, plasma total calcium or whole body calcium efflux. 25-OH-D₃ at the same dose had no effect on any of the parameters tested and none of the seco-steroids tested had any effect on plasma total inorganic phosphate. These data show that both D₃ and 1,25-(OH)₃-D₃ can have calcitropic effects in this marine teleost and that these two forms of vitamin D can exert different effects within the same species.     

25％吡蚜酮悬浮剂防治稻飞虱药效研究

对25％吡蚜酮悬浮剂（24mL佑67m2）与常规对照药剂10％吡虫啉可湿性粉剂（25g／667m2）防治早稻稻飞虱进行药效试验．评价25％吡蚜酮悬浮剂对稻飞虱的防治效果。结果表明：25％吡蚜酮悬浮剂对稻飞虱有较好的防效,可提高水稻产量．减少施药用工,增加经济效益。     

Relation of Vitamin D Status to Congestive Heart Failure and Cardiovascular Events in Dogs

Background

Vitamin D plays a pivotal role in cardiac function, and there is increasing evidence that vitamin D deficiency is associated with the development of congestive heart failure (CHF) in people.

Hypothesis

Serum vitamin D concentration is lower in dogs with CHF compared with unaffected controls and serum vitamin D concentration is associated with clinical outcome in dogs with CHF.

Animals

Eighty‐two client‐owned dogs.

Methods

In this cross‐sectional study, we examined the association between circulating 25‐hydroxyvitamin D [25(OH)D], a measure of vitamin D status, and CHF in dogs. In the prospective cohort study, we examined whether 25(OH)D serum concentration was associated with clinical outcome in dogs with CHF.

Results

Mean 25(OH)D concentration (100 ± 44 nmol/L) in 31 dogs with CHF was significantly lower than that of 51 unaffected dogs (123 ± 42 nmol/L; P = .023). The mean calculated vitamin D intake per kg of metabolic body weight in dogs with CHF was no different from that of unaffected dogs (1.37 ± 0.90 μg/kg metabolic body weight versus 0.98 ± 0.59 μg/kg body weight, respectively, P = .097). There was a significant association of serum 25(OH)D concentration on time to clinical manifestation of CHF or sudden death (P = .02).

Conclusion and Clinical Relevance

These findings suggest that low concentrations of 25(OH)D may be a risk factor for CHF in dogs. Low serum 25(OH)D concentration was associated with poor outcome in dogs with CHF. Strategies to improve vitamin D status in some dogs with CHF may prove beneficial without causing toxicity.     

Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench

A Micromonospora strain, isolate MT25^T, was recovered from a sediment collected from the Challenger Deep of the Mariana Trench using a selective isolation procedure. The isolate produced two major metabolites, n-acetylglutaminyl glutamine amide and desferrioxamine B, the chemical structures of which were determined using 1D and 2D-NMR, including ¹H-¹⁵N HSQC and ¹H-¹⁵N HMBC 2D-NMR, as well as high resolution MS. A whole genome sequence of the strain showed the presence of ten natural product-biosynthetic gene clusters, including one responsible for the biosynthesis of desferrioxamine B. Whilst 16S rRNA gene sequence analyses showed that the isolate was most closely related to the type strain of Micromonospora chalcea, a whole genome sequence analysis revealed it to be most closely related to Micromonospora tulbaghiae 45142^T. The two strains were distinguished using a combination of genomic and phenotypic features. Based on these data, it is proposed that strain MT25^T (NCIMB 15245^T, TISTR 2834^T) be classified as Micromonospora provocatoris sp. nov. Analysis of the genome sequence of strain MT25^T (genome size 6.1 Mbp) revealed genes predicted to responsible for its adaptation to extreme environmental conditions that prevail in deep-sea sediments.     

玉米和毛苕子间种轮作对土壤水分及硬度的影响

为探讨玉米与绿肥作物间种轮作后土壤物理性质的变化,对玉米与毛苕子的间种轮作模式进行试验以明确间种绿肥作物并翻压绿肥对土壤水分和硬度的影响.结果表明:6月25日3个模式0~20 cm 土层土壤含水量在14.9%~17.5%;20~60 cm 土层土壤含水量在18.5%~23.0%;7月27 日 0~60 cm 土层以模式一土壤平均含水量最低,为14.1%,模式二的为14.8%,模式三的最高,为16.2%;9月25日,0~60 cm 土层土壤平均含水量达到了25%左右.模式一和模式二0~30 cm 土层的土壤硬度均小于不种绿肥的模式三.说明玉米、绿肥作物间种并翻压绿肥能够很好地改善土壤的物理性质,提升土壤自身的蓄水保墒能力.     

25-OH-D3对42~63日龄黄羽肉鸡屠宰性能、肉品质和维生素D代谢产物的影响

以低钙、磷水平为基础设计日粮,研究日粮中添加25-OH-D3对42~63日龄黄羽肉鸡屠宰性能、肉品质和VD代谢产物的影响。试验选用42日龄黄羽肉公鸡1200只,随机分成4组,每组5个重复,每个重复60只鸡。处理组1~4中25-OH-D3的添加水平分别 为0、50、70和90μg/kg。结果表明,相对于对照组,饲粮中添加不同水平的25-OH-D3对42~63日龄黄羽肉鸡的屠宰性能影响不显著;可提高内脏器官指数袁促进内脏器官发育,但90μg/kg剂量对外周免疫器官发育有抑制趋势;25-OH-D3对黄羽肉鸡肉色、系水力和嫩度具有一定的改善作用,对pH值影响不显著;血清和肾脏1,25-(OH)2-D3显著升高,且与25-OH-D3添加量成正相关性,肝脏25-OH-D3含量变化不显著。结果显示,降低日粮钙磷水平,日粮中添加25-OH-D3对后期黄羽肉鸡内脏器官发育和肉品质具有一定改善作用,其中70μg/kg25-OH-D3添加量的效果最佳。