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Several diazotrophic Paenibacillus strains were isolated from extracts of surface-sterilized lodgepole pine seedling and tree tissues. One strain, Paenibacillus polymyxa P2b-2R, was found to fix high amounts of nitrogen when reintroduced to the gymnosperms, lodgepole pine and western red cedar. We wanted to determine if P. polymyxa P2b-2R could colonize rhizosphere and internal tissues, fix N and promote growth of corn (Zea mays L), an important agricultural crop. We inoculated corn seeds with P. polymyxa strain P2b-2R and grew seedlings for 30 days. Corn seedlings were harvested 10, 20 and 30 days after inoculation for evaluation of endophytic and rhizospheric colonization as well as nitrogen fixation and growth promotion. P2b-2R successfully colonized the rhizosphere as well as internal root tissues of corn (i.e., endophytically) with population densities near 106 cfu. Corn seedling growth was promoted significantly by inoculation with P2b-2R with an increase of up to 35% in length and up to 30% in biomass after 30 days of inoculation. Seedlings inoculated with P2b-2R derived up to 20% of foliar nitrogen from atmosphere after 30 days of inoculation, which is significant considering the fact that this was a short growth trial. These results suggest that P. polymyxa P2b-2R might have a broad range of plant hosts and is able to fix N and promote the growth of at least one important agricultural crop i.e. Corn. 相似文献
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Hydrophobic chlorinated hydrocarbons are adsorbed onto particles in the environment. Thus, uptake of chlorinated hydrocarbons into organisms is likely to be from the particle-adsorbed state. We investigated the transfer of chlorinated hydrocarbons from particles to phospholipid membranes, quantified by quenching of the fluorescence of a carbazole-labeled phospholipid which was used to label the membranes. The chlorinated hydrocarbons studied included DDT, DDE, methoxychlor, lindane, stirofos, and several polychlorinated biphenyls. The particles studied were porous glass, kaolinite, cellulose, and silica. Transfer of chlorinated hydrocarbons from particles to vesicles was independent of the chlorinated hydrocarbon concentration. The transfer rate did not depend on binding of lipids to particles, and was independent of the lipid composition of the vesicles. Membrane uptake of chlorinated hydrocarbons from the particle-adsorbed state was more rapid than uptake from the microcrystalline state, and uptake was faster when the chlorinated hydrocarbons were dispersed over larger particle surface areas. The transfer rates of chlorinated hydrocarbons correlated with their aqueous solubilities. In total, these results indicate that chlorinated hydrocarbons are transferred through the aqueous phase. We judge the rate-limiting step in membrane uptake to be the rate of solubilization of the chlorinated hydrocarbons from the particles into the bulk aqueous phase. Adsorption of chlorinated hydrocarbons onto particles may enhance the transfer rate by dispersing the chlorinated hydrocarbons and making them more available for solubilization in the bulk aqueous phase. The data are correlated with a model that assumes diffusion across an unstirred aqueous layer which interfaces with the bulk aqueous phase and the particle. 相似文献
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以大花香水月季(Rosa odoratavar. gigantea)为材料,研究了影响愈伤组织诱导和分化的因素,包括不同生长调节剂浓度和组合、暗培养时间等。结果表明,以带有腋芽的茎段为外植体,MS为基本培养基,腋芽萌发的最适激素浓度组合为6-BA 1.5mg/L+NAA0.12mg/L;最适诱导愈伤培养基为MS+2,4-D 4.0mg/L,诱导率为93.33%;最适诱导不定芽培养基为MS+TDZ 3.0mg/L+GA3 1.0mg/L+NAA 0.1mg/L,暗培养15d,再生率为18%。本研究通过间接器官再生途径初步建立的大花香水月季植株再生体系,为利用外源基因对月季进行遗传改良奠定了基础。 相似文献
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甘孜州植物病虫害防治,事关长江流域乃至全国的生态安全和环境安全,鉴于其发展历程和现存问题,甘孜人更易接受和采用与国外IPM(有害生物综合治理)基本一致的"预防为主,综合防治"策略。具体对策有:准确理解和贯彻落实我国"预防为主,综合防治"的植保方针;利用高新科技培育抗病虫害品种并加以合理应用;大力开发运用生物防治方法;改良化学防治,使之与可持续农业相适应;建立植物病虫害防治信息网络;积极引进和培养专业植保人才,大力提高农民的防治水平;加强植物病虫害基础科学研究,不断提高综合防治的科技含量;搞好综合防治方案的设计;做好综合防治效果的评估。 相似文献
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