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951.
There is now clear evidence for a prolonged increase in atmospheric CO2 concentrations and enrichment of the biosphere with N. Understanding the fate of C in the plant-soil system under different CO2 and N regimes is therefore of considerable importance in predicting the environmental effects of climate change and in predicting the sustainability of ecosystems. Swards of Lolium perenne were grown from seed in a Eutric Cambisol at either ambient (ca. 350 μmol mol−1) or elevated (700 μmol mol−1) atmospheric pCO2 and subjected to two inorganic N fertilizer regimes (no added N and 70 kg N ha−1 month−1). After germination, soil solution concentrations of dissolved organic C (DOC), dissolved inorganic N (DIN), dissolved organic N (DON), phenolics and H+ were measured at five depths down the soil profile over 3 months. The exploration of soil layers down the soil profile by roots caused transient increases in soil solution DOC, DON and phenolic concentrations, which then subsequently returned to lower quasi-stable concentrations. In general, the addition of N tended to increase DOC and DON concentrations while exposure to elevated pCO2 had the opposite effect. These treatment effects, however, gradually diminished over the duration of the experiment from the top of the soil profile downwards. The ambient pCO2 plus added N regime was the only treatment to maintain a notable difference in soil solution solute concentration, relative to other treatments. This effect on soil solution chemistry appeared to be largely indirect resulting from increased plant growth and a decrease in soil moisture content. Our results show that although plant growth responses to elevated pCO2 are critically dependent upon N availability, the organic chemistry of the soil solution is relatively insensitive to changes in plant growth once the plants have become established. 相似文献
952.
To evaluate the pathways and dynamics of inorganic nitrogen (N) deposition in previously N-limited ecosystems, field additions of 15N tracers were conducted in two mountain ecosystems, a forest dominated by Norway spruce (Picea abies) and a nearby meadow, at the Alptal research site in central Switzerland. This site is moderately impacted by N from agricultural and combustion sources, with a bulk atmospheric deposition of 12 kg N ha−1 y−1 equally divided between NH4+ and NO3−. Pulses of 15NH4+ and 15NO3− were applied separately as tracers on plots of 2.25 m2. Several ecosystem pools were sampled at short to longer-term intervals (from a few hours to 1 year), above and belowground biomass (excluding trees), litter layer, soil LF horizon (approx. 5-0 cm), A horizon (approx. 0-5 cm) and gleyic B horizon (5-20 cm). Furthermore, extractable inorganic N, and microbial N pools were analysed in the LF and A horizons. Tracer recovery patterns were quite similar in both ecosystems, with most of the tracer retained in the soil pool. At the short-term (up to 1 week), up to 16% of both tracers remained extractable or entered the microbial biomass. However, up to 30% of the added 15NO3− was immobilised just after 1 h, and probably chemically bound to soil organic matter. 16% of the NH4+ tracer was also immobilised within hours, but it is not clear how much was bound to soil organic matter or fixed between layers of illite-type clay. While the extractable and microbial pools lost 15N over time, a long-term increase in 15N was measured in the roots. Otherwise, differences in recovery a few hours after labelling and 1 year later were surprisingly small. Overall, more NO3− tracer than NH4+ tracer was recovered in the soil. This was due to a strong aboveground uptake of the deposited NH4+ by the ground vegetation, especially by mosses. 相似文献
953.
954.
César Valenzuela-Solano David M. Crohn James A. Downer 《Biology and Fertility of Soils》2005,41(1):38-45
Consistent use of mulches over several years can provide significant N to avocado. Study of a 3-year-old Ventura, California, avocado orchard mulched annually for 3 years with 12–14 Mg ha–1 chipped eucalyptus showed that total N in the mulched soil was double that of the control. Mulched intact soil cores released 53 kg ha–1 more N annually than control treatments. A litterbag study showed that net mineralization of the applied mulch commenced approximately 8.5 months following application. Mulched soils tended to be warmer and moister than control soils and temperatures varied less. Laboratory incubations of mulch and soil layers showed that net mineralization rates (mg kg–1 day–1) were greatest in the lowest decomposed mulch layer, but that more N mineralized overall (g m2) in the soil due to its greater density. 相似文献
955.
956.
Shahbaz Khan Hafeez ur Rehman Muhammad Ashfaq Wahid Muhammad Farrukh Saleem Mumtaz Akhtar Cheema Shahzad Maqsood Ahmed Basra 《Journal of plant nutrition》2016,39(12):1681-1687
The impact of soil (1, 2 kg ha?1) and foliar (100, 200 mg L?1) boron (B) with control (no B) was evaluated on phenology and yield formation of Camelina each applied at stem elongation and flowering stages. Foliar (200 mg L?1) or soil B (2 kg ha?1) resulted in earlier flowering and maturity, increased fruit bearing branches (19.68%), number of siliqua, seeds per siliqua (4.6%), biological yield (15%), seed yield (24%), harvest index (11.4%) and oil contents (23%) than no B. Increased fruit bearing branches, seed filled siliqua or seed numbers, harvest index and oil quality can be attributed to changes in dry matter accumulated of stem with simultaneous increase in siliqua dry weight with foliar or soil applied B. In crux, foliar (200 mg L?1) or soil applied (2 kg ha?1) B seems promising to improve seed and oil yield, harvest index of Camelina sativa under B deficient condition. 相似文献
957.
添加硝化抑制剂DMPP对红壤水稻土硝化作用及微生物群落功能多样性的影响 总被引:2,自引:0,他引:2
通过室内培育试验,研究了不同施氮水平下添加硝化抑制剂(DMPP)处理对红壤水稻土NH4+-N、NO3–-N含量、微生物生物量碳及微生物群落功能多样性的影响。结果表明:56天培养期内,不同处理的NH4+-N含量总体呈下降趋势,而NO3–-N含量呈上升趋势。随施氮水平提高,培养期内NH4+-N平均含量从0 mg/kg处理的24.10 mg/kg增加到400 mg/kg处理的412.10 mg/kg,NO3–-N平均含量从0 mg/kg处理的41.88 mg/kg增加到400 mg/kg处理的99.83 mg/kg。添加DMPP显著抑制硝化作用进行,抑制效果随施氮量增加而提高,400 mg/kg施氮水平下,添加DMPP硝化率和硝化速率比不添加DMPP处理分别下降了29.0%和44.3%,下降幅度远大于其他施氮水平处理。施氮水平也影响土壤微生物生物量碳和微生物群落功能多样性。施氮量从0 mg/kg增加到400 mg/kg,土壤微生物生物量碳下降了12.5%,AWCD值下降了78.4%,Shannon指数下降了22.3%;与不添加DMPP处理相比,添加DMPP处理的土壤微生物生物量、AWCD值、Shannon指数分别提高了2.1%、23.9%、7.8%,尤其在400 mg/kg施氮水平下,提高的幅度更加明显。 相似文献
958.
TheA-value method, involving the application of a higher15N rate to a reference non-N2-fixing plant, was used to assess the magnitude of N2 fixation in two bambara groundnut cultivars at four growth stages [vegetative, 0–47 days after planting (DAP); early pod-filling, 47–99 DAP; mid-pod-filling, 99–120 DAP; physiological maturity, 120–148 DAP). The cultivars were Ex-Ada, a bunchy type, and CS-88-11, a slightly spreading type. They were grown on a loamy sand. Uninoculated Ex-Ada and CS-88-11 were used as reference plants to measure the N2 fixed in the inoculated bambara groundnuts. In this greenhouse study, soil was the major source of N in bambara groundnuts during vegetative growth, and during this period it accounted for over 80% of the N accumulaed in the plants. However, N2 fixation became the major source of plant N during reproductive growth. There were significant differences between the two cultivars in the ability to fix N2, and at physiological maturity, almost 75% of the N in CS-88-11 was derived from the atmosphere compared to 55% in Ex-Ada. Also, the total N fixed in CS-88-11 at physiological maturity was almost double that in Ex-Ada. Our data indicate that the higher N2 fixation in CS-88-11 was due to two factors, a higher intensity of N2 fixation and a longer active period of N2 fixation. The results also suggest that bambara groundnut genotypes could be selected for higher N2 fixation in farining systems. 相似文献
959.
The effects of wheat straw and different forms of N on denitrification and N immobilization were studied in an anaerobic water-sediment system. The water-sediment system was supplemented with various combinations of wheat straw and 15N-labelled and unlabelled (NH4)2SO4 or KNO3, and incubated anaerobically at 30°C for 10 days. 15N-labelled and unlabelled NO
inf3
sup-
, NO
inf2
sup-
, NH
inf4
sup+
, and organic N were determined in the water-sediment system. The gases evolved (N2, CO2, N2O, and CH4) were analyzed by gas chromatography at regular intervals. Larger quantities of 15N2–N and organic 15N were formed in wheat straw-amended systems than in non-amended systems. Trends in CO2 production were similar to those of N2–N evolution. The evolution of N2O and CH4 was negligible. Denitrification processes accounted for about 22 and 71% of the added 15NO
inf3
sup-
–N in the absence and presence of wheat straw, respectively. The corresponding denitrification rates were 3.4 and 12.4 g 15Ng-1 dry sediment day-1. In systems amended with 15NO
inf3
sup-
–N and 15NO
inf3
sup-
+NH
inf4
sup+
–N without wheat straw, 1.82 and 1.58%, respectively, of the added 15NH
inf3
sup-
–N was immobilized. The corresponding figures for the same systems supplemented with wheat straw were 5.08 and 4.10%, respectively. Immobilization of 15NO
inf4
sup+
–N was higher than that of 15NO
inf3
sup-
–N. The presence of NO
inf3
sup-
–N did not stimulate NH
inf4
sup+
–N immobilization. 相似文献
960.
Mariangela Hungria Joan M. Ellis Ralph W. F. Hardy Allan R. J. Eaglesham 《Biology and Fertility of Soils》1993,15(3):208-214
Summary Bradyrhizobial strain BTAi 1 nodulates both stems and roots of Aeschynomene spp. Previous work has shown that it contains bacteriochlorophyll a and forms photosynthetic reaction centers, and has provided indirect evidence of photosynthesis by bacteroids within stem nodules. Here we report physiological and biochemical characteristics of BTAi 1 ex planta, which also suggest the presence of photosynthetic activity. Light-stimulated uptake of 14CO2 by BTAi 1 was detected at all stages of growth. Inhibitors of photosynthesis, 1,10-orthophenanthroline and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), and the uncoupler NH4Cl, immediately suppressed light-driven 14CO2 uptake and increased O2 uptake. BTAi 1 is strictly aerobic and was unable to grow without organic C even in the light; also, it was unable to grow chemoautotrophically in an atmosphere enriched with H2 and CO2. In micro-aerobic conditions, strain BTAi 1 expressed acetylene reducing activity ex planta in an N-free medium. The highest rates of light-stimulated 14CO2 uptake and acetylene-reducing activity occurred during the exponential and early stationary phases of growth. Acetylene-reducing rates at a low glucose concentration were increased following a light-dark cycle in comparison with continuous dark conditions. 相似文献