全文获取类型
收费全文 | 5154篇 |
免费 | 235篇 |
国内免费 | 441篇 |
专业分类
林业 | 240篇 |
农学 | 367篇 |
基础科学 | 130篇 |
781篇 | |
综合类 | 1962篇 |
农作物 | 309篇 |
水产渔业 | 203篇 |
畜牧兽医 | 1111篇 |
园艺 | 417篇 |
植物保护 | 310篇 |
出版年
2024年 | 11篇 |
2023年 | 83篇 |
2022年 | 139篇 |
2021年 | 179篇 |
2020年 | 163篇 |
2019年 | 175篇 |
2018年 | 136篇 |
2017年 | 223篇 |
2016年 | 217篇 |
2015年 | 206篇 |
2014年 | 290篇 |
2013年 | 359篇 |
2012年 | 374篇 |
2011年 | 427篇 |
2010年 | 355篇 |
2009年 | 338篇 |
2008年 | 307篇 |
2007年 | 317篇 |
2006年 | 283篇 |
2005年 | 196篇 |
2004年 | 179篇 |
2003年 | 111篇 |
2002年 | 107篇 |
2001年 | 84篇 |
2000年 | 92篇 |
1999年 | 90篇 |
1998年 | 49篇 |
1997年 | 47篇 |
1996年 | 50篇 |
1995年 | 40篇 |
1994年 | 21篇 |
1993年 | 31篇 |
1992年 | 36篇 |
1991年 | 24篇 |
1990年 | 17篇 |
1989年 | 19篇 |
1988年 | 13篇 |
1987年 | 11篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1974年 | 1篇 |
1956年 | 4篇 |
1955年 | 2篇 |
排序方式: 共有5830条查询结果,搜索用时 15 毫秒
121.
AIM: To investigate the correlation of gene modification between histone H3 lysine 4 (H3K4) trimethylation and DNA methylation in IgA nephropathy patients. METHODS: H3K4 trimethylation variations were analyzed in peripheral blood mononuclear cells (PBMCs) from 40 IgAN patients and 40 healthy controls by the method of chromatin immunoprecipitation linked to microarrays (ChIP-chip). ChIP real-time PCR was used to validate the microarray results. Quantitative real-time PCR (qRT-PCR) was employed to analyze mRNA expression. DNA methylation in 4 selected positive genes was analyzed by the method of methylated DNA immunoprecipitation-quantitative PCR (MeDIP-qPCR). RESULTS: IgAN patients displayed higher H3K4 trimethylation level and lower DNA methylation level than those of the healthy controls. There were significant differences in DNA methylation and H3K4 trimethylation of 4 selected genes between IgAN patients and the healthy controls (P<0.05). CONCLUSION: Our studies indicate that significant alterations of H3K4 trimethylation and DNA methylation exist in IgAN patients. There is a correlation of gene modification between DNA mathylation and histone H3 lysine 4 trimethylation in IgAN patients. 相似文献
122.
Plants display an array of classical strategies to maximize phosphate (Pi) acquisition from sparingly soluble P sources. Acclimation to Pi-stress via elegant Pi-starvation induced (PSI) adjustments would reduce our current overreliance on expensive, polluting and non-renewable Pi-fertilizers. Nevertheless, differences in the ability of various species to solubilize sparingly soluble P-sources have been often evidenced; inter-cultivar variations are scarcely documented. Brassica is known as an effective, non-mycorrhizal user of sparingly soluble P-sources. Various growth parameters and biomass accumulation by genetically diverse Brassica cultivars were determined in four experiments using hydroponics and quartz sand culture media. Role of PSI root mediated pH changes, organic anions (OAs) exudation and altered root architecture in mobilization and acquisition of sparingly soluble P-forms [Jordan rock-P (RP) at 2 g l−1 and Ca3(PO4)2 (TCP) at 0.2 g l−1 respectively] was investigated. Cultivars showed considerable genetic variations in biomass accumulation, various growth parameters and root–shoot ratio. Concentration and total uptake of P, specific absorption rate of P, P-transport rate and P-utilization efficiency (PUE) were also significantly (P < 0.001) different for various cultivars and their dry matter was significantly correlated with P-uptake [r = 0.94** (significant at 1% level)]. P-tolerant cultivars showed substantial decrease in solution media pH because of H+ efflux and exuded more carboxylates than low P-sensitive cultivars under P-starvation. P-uptake by cultivars increased linearly with decreasing pH. The amount and types of OAs exuded from the roots of P-starved plants differed from those of plants grown under P-sufficient environment. In split pot study, with TCP and RP supplied spatially separated from other nutrients, efficient cultivars were still able to mobilize RP and TCP more efficiently than inefficient cultivars. In rhizobox study, the elongation rates of primary roots decreased but the elongation rates of the branched zones of primary roots and the length of lateral roots increased under P-starvation. Tested cultivars showed genetic diversity in accessing, mobilization, acquisition and utilization of Pi from sparingly soluble P forms. An arrange marriage of plant traits can explain cultivar’s access to different forms of sparingly soluble P, and in addition to altered lateral root topology and enhanced P-uptake and PUE, enhanced H+ efflux and OAs exudation are key factors in Pi scavenging from extra cellular sparingly soluble P-forms. 相似文献
123.
本文报导了一个新的大豆斑驳粒微量“总黄酮”的分析方法,。采用Zr^4+与黄酮类化合物生成有荧光的络合物,借薄层色谱扫描技术,测定滤纸荧光斑点的峰面积积分值,用于定量分析。Zr^4+在酸性条件下与黄酮类化合物的络合作用可使类黄酮化合物定性,并有利于区别于黄烷醇类化合物。该方法应用盱大豆感染SMV产生斑驳的发生机理研究,有良好的效果。 相似文献
124.
在低刈条件下狗牙根419繁育性的研究 总被引:1,自引:1,他引:0
刈割高度狗牙根419孕穗率和种子成熟度有严重影响,且留槎高度越低,影响越严重。在低刈条件下,花序轴产生2种生长向,即匍匐型和超缩短的集合茎。 相似文献
125.
高温条件下复合抗应激剂对肉鸡的影响 总被引:5,自引:0,他引:5
本文研究了高温条件下复合抗应激剂对肉鸡的影响。结果表明:经历32 ℃一周的5 ~8 周龄肉鸡采食量、增重、饲料转化率均显著下降(P< 0 .01) ,饮服复合抗应激剂显著改善增重和饲料转化率(P< 0 .01) , 采食量未见明显改变,饲料转化率达到常温水平。32 ℃16 小时血浆皮质酮、T3 显著下降(P< 0 .01) 、T4 显著升高(P< 0 .01) ;32 ℃120 小时血浆皮质酮、T3 升高并超出常温组(P< 0 .05) 、T4 较16 小时下降但仍高出常温组(P< 0 .05) 。饮服复合抗应激剂时血浆皮质酮、T3 、T4 显现相同变化规律,但这种变化得到显著缓解 相似文献
126.
探讨不同时期山羊睾丸组织中干细胞的分布和特点,以45 d和75 d山羊胎儿及出生后4月龄山羊睾丸为试验材料,进行了HE染色和Oct4抗体的免疫组化染色。结果显示,在45 d山羊胎儿睾丸组织中睾丸实质细胞聚集呈团状,形成岛屿状组织结构;发育到75 d山羊胎儿睾丸组织中睾丸的间质与实质组织结构差异明显,睾丸生精小管已经形成,生精小管管腔闭锁;出生后4月龄山羊睾丸组织结构明显,生精小管形态特征清晰,但管腔仍处于闭锁状态;Oct4免疫组织化学显示在45 d山羊胎儿睾丸组织中Oct4阳性细胞呈岛屿状分布,多见于生精小管前期的组成细胞;对于75 d山羊胎儿睾丸组织Oct4阳性细胞呈环状分布于生精小管中,睾丸间质组成细胞中则较少;在出生后4月龄山羊睾丸中阳性细胞主要分布于睾丸组织生精小管基底膜,沿基底膜环状分布。试验证实了在早期山羊睾丸组织中Oct4主要表达在生精前体细胞。 相似文献
127.
在介绍Exendin-4的基础上,综述了目前最新的Exenatide用于治疗糖尿病的研究进展,并基于大量临床前数据,阐释了其功能、作用机制和毒理学研究。 相似文献
128.
本试验以装有瘤胃瘘管的3头荷斯坦奶牛为试验动物,利用体外批次培养试验法.研究了在奶牛三种不同品质的粗饲料日粮中分别添加不同浓度的戊酸(1.25、2.50、5.00、10.00mg/g)对其体外发酵pH、氨氮(NH3-N)浓度和气体产量的影响.结果表明:(1)培养液pH值均随着培养时间的延长呈逐渐下降的趋势,但各试验组和对照组均值差异不显著(P>0.05).(2)培养液NH3-N浓度的变化趋势基本一致.随着培养时间的延长,培养液NH3-N浓度在3~6 h降低,9~24 h又逐渐升高.(3)培养液气体产量的变化均随培养时间的延长呈逐渐的上升趋势. 相似文献
129.
LI Mengwei PENG Lijuan PENG Kaiping XIE Fang LIANG Xin GUO Yangxia YANG Chengjian 《中国畜牧兽医》2007,47(9):2767-2778
This study was aimed to evaluate the effects of inhibiting rumen bacteria,fungi and protozoa with adding linoleic acid and linolenic acid on in vitro rumen fermentation and fatty acid metabolism in buffaloes.Both fatty acids were supplemented with substrate and roughage (3:7) at the rate of 3% on dry matter (DM) basis in an in vitro batch culture system,there were 5 repetitions for each group.At the same time,four groups were set up:Control group and inhibition groups of protozoa,bacteria and fungi.After 24 h of incubation,total gas production,CH4,pH,VFA,NH3-N,MCP and LFA concentrations were measured.The results showed that:①With the addition of linolenic acid,compared with control group,the gas production decreased significantly after inhibition the growth of bacteria and protozoa,CH4 production increased significantly after inhibition of the growth bacteria and fungi,and CH4 production decreased significantly after inhibition of the growth protozoa (P<0.05).With the addition of linoleic acid,compared with control group,the gas production decreased significantly after inhibiting the growth of bacteria,fungi or protozoa,and CH4 production was significantly lower than other groups after inhibition of protozoa (P<0.05).② After inhibiting the growth of bacteria,fungi or protozoa,the pH and MCP concentration were affected significantly with the addition of linolenic acid (P<0.05),there was no significant effect on NH3-N concentration with the addition of linoleic acid (P>0.05).③ Compared with control group,the content of acetic acid and propionic acid was reduced significantly after inhibiting the growth of bacteria,fungi or protozoa (P<0.05).The butyric acid was reduced significantly after inhibiting the growth of bacteria with the addition of linolenic acid (P<0.05).The butyric acid was reduced significantly after inhibiting the growth of bacteria,fungi or protozoa with the addition of linoleic acid (P<0.05).④ Compared with control group, the concentrations of C11:0, C12:0, C13:0, C14:0, C14:1n5, C15:1n5, C16:1n7, C16:0, C18:3n3, C18:2n6c, C18:0, C20:2n6, C20:3n6, C20:1, C20:3n3, C20:0, C21:0, C22:6n3, C22:2n6, C22:0 was reduced significantly after inhibiting the growth of bacteria with the addition of linolenic acid, the concentrations of C12:0, C13:0, C14:0, C15:0, C16:1n7, C16:0, C17:0, C18:3n6, C18:3n3, C18:2n6c, C18:1n9t, C18:0, C18:2(cis-9,trans-11), C18:2(trans-10,cis-12), C20:2n6, C20:1, C20:0, C21:0, C22:6n3, C22:0, C23:0, C24:1n9, C24:0 was reduced significantly after inhibiting the growth of bacteria with the addition of linoleic acid (P<0.05).The results revealed that the addition of linoleic acid and linolenic acid could significantly manipulate in vitro rumen fermentation parameters,CH4 yield and fatty acid composition after inhibiting the growth of bacteria,fungi or protozoa.Protozoa greatly contributed to total gas and CH4 production while bacteria significantly affected rumen fatty acid metabolism. 相似文献
130.
从江泽民同志“四个同样重要”理论出发,结合云南农业大学大学精神建设、社会科学学科建设、学报社科版建设以及校园文化建设等方面的基本情况,论证了进一步解放思想对于繁荣高校哲学社会科学的重要意义。 相似文献