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951.
黄芪多糖对雏鸡外周血T淋巴细胞转化功能的影响 总被引:37,自引:3,他引:34
将108只1日龄伊莎系蛋用公雏均分为3组:一组为对照,其余2组在3日龄时,于背侧颈部皮下分别注射0.2、0.4mL黄芪多糖注射液(0.01g/mL)1次,再分别于7、21、35、49日龄时采用MTT比色法及微量全血培养3H-TdR掺入法检测外周血T淋巴细胞转化率的动态变化。结果表明,黄芪多糖对21、35日龄雏鸡T淋巴细胞转化功能有增强作用,且与剂量有相关性,而对7、49日龄雏鸡的作用不明显。MTT比色法与3H-TdR掺入法的检测结果无显著差异(P<0.05)。 相似文献
952.
以提取的血清1型鸭疫里氏杆菌(R.a)Jb2株的脂多糖作为包被抗原,成功地建立了一种检测1型R.a抗体的间接ELISA方法,特异性试验和重复性试验证明此方法的特异性高、重复性好。并比较了间接ELISA方法、间接血凝试验、玻片凝集试验和琼脂扩散试验对抗体检出的敏感性,结果表明间接ELISA方法最敏感,人工感染后的鸭第72小时即可检出血清抗体为阳性(3/4);其次是间接血凝试验,第96小时即可检出50%(2/4);玻片凝集试验在感染9天后才出现阳性(2/3);而琼扩试验在感染后11天仍为阴性反应。应用建立的间接ELISA方法,初步调查了南京地区鸭场此病的感染情况,发现所调查的13日龄以前雏鸭的阳性率为0,而21~35日龄鸭的阳性率为873%。 相似文献
953.
鸡传染性法氏囊病(IBD)和新城疫(ND)病毒接种同一鸡胚收取种毒试制IBD、ND二联弱毒冻干疫苗,并按《规程》方法分别检验。结果证明安全、效力均能达到合格标准,两种病毒互相不干扰抗体的产生。 相似文献
954.
黄瓜种质资源遗传亲缘关系的RAPD分析 总被引:82,自引:6,他引:76
利用RAPD技术对多个生态型的黄瓜材料的遗传亲缘关系进行了研究。从200个10碱基随机引物中筛选出20个用于PCR反应,39.2%的扩增条带表现多态性;每个生态型品种都具有其特有的扩增(缺失)带以区别其他生态型品种;聚类分析将供试材料分为3大类群:华北类群、华南类群和欧洲温室类群。从分子水平验证了传统的黄瓜地域分类标准及黄瓜是遗传基础狭窄的蔬菜作物。对利用RAPD技术进行亲缘控制研究的可能性进行了探讨。 相似文献
955.
Abstract – Describing habitat use by stream fishes is important from both basic ecological and fisheries management points of view. The most widely used methods of measuring habitat use vary in degree of effort required, level of intrusiveness and in the level of spatial and temporal resolution. In this paper, we describe a remote monitoring technique that can provide detailed and continuous data on habitat use of individual fish in the field. The technique is based on the passive integrated transponder (PIT) system, in which a newly developed flat-bed antenna is placed on the stream bottom and simply requires a PIT-tagged fish to swim over it. We present data obtained from work using this new technology on brown trout ( Salmo trutta ) in stream enclosures, in which we describe habitat use and temporal patterns of movement by individuals and relate such data to growth rate and sex of the individual fish as well as to pool depth and time of day. In addition, we describe the range of applications of the flat-bed PIT-antenna as well as the advantages and disadvantages of using the system. NOTE 相似文献
956.
E.coli K1 strain is a representative strain of neonatal sepsis and meningitis, which causes disease by blood circulation to the brain. The molecular mechanism of E.coli K1 that adhering and invading the brain microvascular endothelial cells (BMECs) and cross the blood brain barrier (BBB) has been focused on by many scholars. In this review, we focused on the gene regulation mechanisms and signaling pathway of E.coli K1 crossing the blood brain barrier to understand the molecular mechanism of the infection caused by E.coli and provide reference for prevention and treatment programs. 相似文献
957.
以杂交马褂木幼林为供试对象,进行不同肥料种类和施肥量试验。结果表明:追肥1a后,各施肥处理间树高生长量有极显著差异,以施洞氮复合肥0.25kg/株和马褂木无机专用肥0.25k∥株的树高生长量最大;追肥3a后,各施肥处理间树高生长量有极显著差异,胸径生长量没有显著差异,以施尿素0.25kg/株的树高生长量最大,胸径和树高生长量分别为3.09em和1.85m,比对照分别提高72%和111%。综合成本效益考虑,适合推广的肥料种类和施肥量依次为马褂木无机专用肥0.1ks/株、马褂木无机专用肥0.25k∥株和尿素0.25kg/株。 相似文献
958.
《Journal of Crop Improvement》2013,27(1):51-65
Abstract Only a few studies have been conducted in which regenerability of barley has been examined. In the current study, 17 barley genotypes (nine two-row malting type: Aster, Emon, Ruen, Jubiley, PV101, Körten, Krassi 2, Perun and Igri, and eight six-row feed types: Karnobat, Hemus, Jerun, Veslets, Aheloi 2, Diana, Panagon, and Izrgev) were evaluated for tissue-culture response from seedlings during a three-year period. Regenerable calli were obtained from all tested genotypes. Although there was much variation in regeneration capacity among the cultivars, plants were obtained from all cultivars. The majority of green plants grown to maturity were fertile and normal in appearance. The frequency of embryogénie callus induction and regenerability was influenced by genotype and growth conditions of the donor plants. Genotype was the most important determinant of the in vitro response. The best in vitro performance, based on ability to form regenerable calli and to regenerate plants was observed for Ruen, Aster, and Emon. 相似文献
959.
《Plant Production Science》2013,16(4):335-341
AbstractThe contribution of cell wall components and nonstructural carbohydrate (NSC) to grain filling in rice (Oryza sativa L.) was clarified by investigating the differences in the dynamics of hemicellulose, sugar composition of hemicellulose, β-(1→3),(1→4)-glucan, and NSC among cultivars with different grain filling capacities. This investigation was performed using the stems of standard, high yield and low harvest index (HI) cultivars. Hemicellulose concentration in stems tended to decrease slightly during the grain filling stage. This decrease was attributed to a decrease in β-(1→3),(1→4)-glucan concentration, which was detected as a decrease in glucose composition of hemicellulose in the stems during the grain filling stage. The rate of decrease and decrease in the amount of β-(1→3),(1→4)-glucan in the stems differed among the cultivars. These were higher in high yield and high HI cultivars than in relatively low yield and low HI cultivars. Moreover, a positive correlation was observed between the rate of decrease in β-(1→3),(1→4)-glucan and NSC, indicating similarities in the dynamics of β-(1→3),(1→4)-glucan and NSC among the cultivars. When the top half of panicle was removed, β-(1→3),(1→4)-glucan and NSC concentrations in the culm and leaf sheath did not decrease during the grain filling stage. Therefore, the β-(1→3),(1→4)-glucan in stems might be one of the sources that supply substrate to panicle as well as NSC. 相似文献
960.
《Plant Production Science》2013,16(3):230-234
AbstractTo elucidate the possible participation of hemicellulose decomposition in lodging resistance, we studied the change of hemicellulose and cellulose content in the stems of rice during the ripening stage by methylation analysis and the expression of related genes by Northern blotting. In the rice stem in ripening stage, content of (1-3,1-4)-β-glucan, a component of hemicellulose, decreased markedly although the content of arabinoxylan, a major component of hemicellulose, and cellulose showed little change during the same growth period. On the other hand, expression of the Gns 1 gene, which may encode (1-3,1-4) -β-glucanase that catalyzes the degradation of (1-3,14) -β-glucan, increased sharply in the stem. The mechanism of decomposition of (1-3,1-4) -β-glucan in rice stem and the possible association with lodging resistance is discussed. 相似文献