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排序方式: 共有232条查询结果,搜索用时 281 毫秒
1.
刺槐外斑尺蠖Ectropis excellens Butler危害刺槐、枣树及农作物,在河南省安阳市一年发生4代,9月下旬至10月上旬以蛹在树干周围1.5m的土层中越冬,翌年4月中旬羽化。鸟类、天敌昆虫等对害虫有较好的控制作用,防治措施为营造混交林、保护天敌、喷施灭幼脲等生物制剂。 相似文献
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Studies were carried out to optimize production of nucleopolyhedrovirus of American bollworm,Helicoverpa armigera (Hübner), by treating larvae individually with an inoculum dose that allowed maximal larval growth and also gave the highest
occlusion bodies (OB) yield/larva. The maximum virus yield of 12.2x 109 OB/larva was obtained when 6-day-old larvae were fed individually with a dose of 1 x 103 OB. Topical spiracular treatment of larvae as old as 8 days with 10 μ of 2x 107 OB ml-1 gave the highest yield, of 15.2x 109 OB from 13-day-old larvae, of 12.8x 108 OB from prepupae and of 1.49x 108 OB from pupae at the time of their death. These studies showed that dietary inoculum is the best route for 6-day-old larvae
and topical spiracular treatment is the best for 8-day-old larvae. 相似文献
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研究结果表明,限食程度越高,茶尺蠖幼虫存活率越低.不论在茶叶或人工饲料上,幼虫忍耐饥饿的能力随龄期的增加而提高,适当限制取食可延长幼虫历期或各龄龄期 相似文献
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通过对家蚕核型多角体病毒(Bombyx mori,nucleopolyhedrovirus:BmNPV)ORF 75基因核苷酸序列进行生物信息学分析,BmNPVORF 75基因位于病毒基因组70 485 bp和71 264 bp之间,编码259个氨基酸残基的多肽,预测分子相对质量为30.8 kDa,等电点为7.67,分子式为C1409H2157N351O390S19;蛋白在大肠杆菌内的半衰期大于10 h;酸性氨基酸(Asp Glu)占10.5%,碱性氨基酸(Arg Lys)占10.8%;蛋白的不稳定指数为42.67,是不稳定蛋白.利用Prosite数据库扫描,查到4类9个蛋白修饰位点.通过Blast搜索氨基酸同源序列发现,BmNPVORF 75同源蛋白存在于所有测序的鳞翅目昆虫杆状病毒中,不存在于其他非鳞翅目昆虫杆状病毒中.同源序列比对结果,BmNPVORF75蛋白的氨基酸序列与苜蓿银纹夜蛾核型多角体病毒(Autographa californica MNPV)ORF92之间的同源性达到100%,应为相同基因.依照BmNPVORF75同源基因序列相似性,对13种昆虫杆状病毒进行进化树构建分析,其中,BmNPVORF75与薄荷灰夜蛾多粒包埋型核型多角体病毒(Rachiplusia ouMN-PV)ORF89基因进化距离很近,同源性较高. 相似文献
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Ebling PM 《Pest management science》2004,60(7):631-638
The biological activity of the Ireland strain of Choristoneura fumiferana (Clem) nucleopolyhedrovirus (CfMNPV) propagated in different hosts was determined to provide the basis upon which genetically modified CfMNPV, or other naturally occurring isolates, should be compared. Occlusion bodies (OB) derived from CF-203 cells were significantly larger and more pathogenic than those propagated in vivo when tested against the fifth larval instar of C fumiferana (Clem) and C occidentalis Freeman. The dose-responses (LD50 and LD95, expressed as occlusion bodies per larva) of C fumiferana larvae to in vitro-propagated OBs were 274 and 5785, respectively. The values of LD50 and LD95 to C occidentalis larvae were 19 and 118, respectively. There were no significant differences in pathogenicity or size when OBs propagated in C fumiferana larvae were tested against either insect species, nor were there significant differences for OBs propagated in C occidentalis larvae. The LD50 and LD95 of in vivo-produced OBs to C fumiferana were 925 and 61988, respectively. The LD50 and LD95 to C occidentalis were 50 and 453, respectively. OBs propagated in vitro had a mean volume of 13.13 microm3, whereas those propagated in vivo ranged from 0.84 to 1.41 microm3. The median survival time-responses (ST50) of fifth-instar C fumiferana or C occidentalis larvae to OBs propagated in vivo were not significantly different from those propagated in vitro at the dosage levels tested. Values of ST50 of C fumiferana larvae to in vitro- and in vivo-produced OBs at dosages causing less than 50% mortality rangedfrom 9.6 to 9.8 days post-inoculation (dpi), whereas a LD95 dose resulted in ST50 values ranging from 7.3 to 7.7 days. ST50 values of C occidentalis larvae at dosages causing less than 50% mortality ranged from 9.8 to 10.2 dpi, whereas a LD95 dose resulted in ST50 values ranging from 9.5 to 9.8 dpi. The median feeding cessation time-response (FT50) of fifth-instar C fumiferana larvae to OBs propagated in vitro (5.7 days) was not significantly different from the FT50 of those propagated in vivo in either insect species (5.3 and 5.7 days) at the dosage level tested (LD95). No significant differences in FT50 values were observed between OBs propagated in either larval host. The FT50 of C occidentalis larvae to OBs propagated in vitro (7.7 days) was not significantly different from that to those propagated in vivo in C occidentalis larvae (7.6days), but somewhat different (7.2 days) from that to those propagated in C fumiferana larvae. Results indicate that CfMNPV can be propagated in vivo in either C fumiferana or C occidentalis larvae (or sequentially through both) without alteration in infectivity, although the use of the CF-203 cell line yields the most biologically active OBs. 相似文献
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13种杀虫剂防治茶尺蠖室内药效试验 总被引:1,自引:0,他引:1
本文报道了13种杀虫剂在室内水培茶树枝条上模拟田间喷雾对茶尺蠖的药效试验。结果表明:乙基多杀菌素60克/升悬浮剂3000倍液、甲胺基阿维茵素苯甲酸盐3%微乳剂15000倍液、甲氧虫酰肼240克/升悬浮剂5000倍液、氰氟虫腙240克/升悬浮剂1500倍液.药后7d的防效均在91%以上,虫酰肼20%悬浮剂1000倍液的防效为86.48%.虽然氟虫双酰胺20%水分散粒剂6000倍液的防效稍低于上述5个处理的防效,但虫粪干重为第二少.其保叶效果仅次于乙基多杀菌素60克/升悬浮剂。因此,乙基多杀菌素60克/升悬浮剂、甲胺基阿维菌素苯甲酸盐3%微乳剂、甲氧虫酰肼240克/升悬浮剂、氰氟虫腙240克/升悬浮剂、虫酰肼20%悬浮剂、氟虫双酰胺20%水分散粒剂是比较理想的防治茶尺蠖的首选药剂.建议在茶园轮换使用。而对照药剂联苯菊酯10%乳油3000倍液对茶尺蠖的防效仅为23%.已不适合用于防治茶尺蠖。 相似文献
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以纯化的茶尺蠖核型多角体病毒(Ectropis obliqua nucleopolyhedrovirus,EcobNPV)作为抗原免疫大耳白兔,获得多克隆抗体,Indirect-ELISA效价为1∶51 200;工作浓度分别为EcobNPV 1∶1 600、兔抗血清1∶3 200。用所制备的多克隆抗体对提纯的多角体进行Western-blot分析表明制得的抗体对茶尺蠖核型多角体病毒有良好的特异性。应用于不同来源茶园昆虫多角体病毒分离株的检测,取得了理想的检测效果。 相似文献