Lighting Design of Chinese Traditional Buildings and Application of New Type of Little Power LED

This paper is a part of the research on the lighting techniques of the traditional Chinese buildings in Summer Palace,Beijing,which is a sub-project of the Municipal Research Project on the Correlative Techniques for Beijing's Sustainable Development(Y0604017040391).The work,which the authors took part in,on the most representative Chinese traditional buildings in Summer Palace,is taken as a case study on lighting design and lab simulated experiments and checked by practical result,illustrating a working frame for other similar lighting design works.     

观赏羽衣甘蓝的离体繁殖

以花蕾为外植体，离体繁殖羽衣甘蓝８个杂各一代材料。培养基为（ｍｇ／Ｌ）：ＭＳ＋ＢＡ１－２＋ＮＡＡ０．１－０．２＋ＩＡＡ０．５＋（或不加）ＧＡ３０．３＋３％蔗糖。平均１８％－６７％的接咎花蕾产生不定芽；药有３０％的不定芽发育成完整植物。培养物的严重褐化和再生植物的普遍玻璃质化是影响植株成活的主要因素。培养基中加入活性炭和青霉素，并给以强光照射，可提高植株成活率。     

赤霉素处理对大白菜阶段发育的影响

用赤霉素处理未通过春化阶段的大白菜，植株可以抽薹开花；赤霉素处理已通过春化阶段的大白菜，可以促进植株提前抽薹开花。大白菜品种不同，赤霉素处理的效果有差异。     

书法用笔在山水画中的美学地位

谙熟中国书画艺术的人知道,中国书法与山水画在用笔方面同源异流却又殊途同归。本文从书法与中国山水画所用之工具、“二王”笔法原则来说明书法用笔对山水画的影响,并以美术史中诸位理论家和画家所述“书画同源”的观点来证明书法用笔与山水画笔法之间的联系,从而阐明书法用笔在山水画中的美学地位。     

Artificial inoculation and susceptibility of Pinus armandii to Dothistroma septosporum

Dothistroma needle blight (DNB) is a serious needle disease of conifers that primarily affects pine species (Pinus spp.). Dothistroma septosporum is one of the DNB pathogens that has a diverse range of host species excluding Pinus armandii. In 15 inoculated P. armandii seedlings, D. septosporum acervuli were observed in 43 infected needles of ten seedlings with a mean disease severity of 1.11% at 25 weeks after inoculations, demonstrating the potential of D. septosporum to cause symptoms on the needles of P. armandii via artificial inoculation. The disease severity of P. armandii was similar to the positive control, Pinus nigra (median 0.75 for P. armandii to 0.70 for P. nigra), thus, P. armandii acts under artificial conditions as a susceptible host species.     

对中国共产党代表中国先进生产力发展要求的思考

只有中国共产党能够代表中国先进生产力的发展要求。本文从先进生产力发展的内在要求出发,论述了代表先进生产力发展要求,必须坚持科学技术是第一生产力,必须高度重视人的因素。     

芥蓝二氢黄酮醇4–还原酶基因BoaDFR的克隆与功能鉴定

以白花芥蓝‘四季粗条’为材料克隆了Boa DFR基因,Gen Bank登录号为MT472647,CDS序列长为1158 bp,编码385个氨基酸,与甘蓝型油菜、甘蓝和白菜等具有高度同源性。Boa DFR表达水平在芥蓝不同发育时期、不同器官中存在显著差异,随植株发育总体呈现先下降后上升的趋势,在幼嫩种子中表达量最高。芥蓝原生质体的亚细胞定位结果显示Boa DFR定位在细胞核上。构建了农杆菌介导的芥蓝瞬时过表达体系,将Boa DFR转入白花芥蓝‘四季粗条’和黄花芥蓝‘福州黄花’中,发现Boa DFR在两种芥蓝中均高水平表达,转基因植株叶片颜色明显变紫,花青素苷显著积累,总含量最高达461.43μg·g-1 FW,而野生型和转空载体芥蓝叶片中几乎检测不到花青素苷。通过HPLC在转基因植株叶片中共检测到8种花青素苷,均为矢车菊花青素苷,其中矢车菊–3–阿魏酰–丙二酰–槐糖苷–5–葡萄糖苷占比最高。     

‘福山包头’大白菜叶柄黑点病发生特点及抗性分析

以‘福山包头’大白菜为试材,利用田间调查、显微观察和叶片离体培养的方法,对‘福山包头’系列大白菜叶柄黑点病的发生特点及抗性进行了研究,以期为改善‘福山包头’大白菜抗病性及新品种选育提供参考依据。结果表明:(1)大白菜叶柄黑点病主要发生于叶柄表皮细胞且起始于细胞周质或细胞壁,该病可发生于大白菜的整个生育期,且进入包心期后发展较快;(2)氮素能诱导叶柄黑点病的发生,且铵态氮较硝态氮诱导效果更明显;(3)氮素浓度与叶柄黑点病的发病程度呈正相关,但氮素并不是影响叶柄黑点病发生的唯一因素;(4)‘福山包头’系列大白菜种质资源中,‘福东2号’‘福山82’对叶柄黑点病的抗性较强,‘福小87-13-13’‘皱福’的抗性较弱。