茄子新品种中丰红茄的选育

中丰红茄是以自交系HY8为母本,以自交系GD6为父本配制而成的茄子一代杂种。中熟,植株生长势较强,耐热,采收期长;分枝性中等,主茎第7~8节着生门茄,果实棍棒形,纵径19.9cm,横径5.6cm。果皮深紫红色,肉色乳白,肉质致密,品质佳;单果质量0.27kg左右。中丰红茄田间对青枯病、黄萎病、炭疽病的抗性强于对照油罐茄和广红茄。每667m2平均产量为4560kg。适合在湖南、湖北、云南、四川、重庆等地栽培。     

小果型西瓜新品种苏蜜8号的选育

苏蜜8号是以SW032为母本,以SW058-1为父本配制而成的小果型西瓜一代杂种。早熟,果实发育期约30d(天)。植株生长势中等,耐低温弱光,易坐果。果实高圆形,单果质量1.8~2.3kg。果皮底色浅绿,覆深绿色窄条带,果皮厚0.4~0.5cm。果肉黄色,中心可溶性固形物含量11.0%~12.2%,质地酥嫩,汁液多,纤维少,风味佳。每667m2产量2000~2500kg,适宜长江中下游地区春季大棚栽培。     

辣椒新品种海丰1052

海丰1052是以08-123为母本,F07-253-2-1为父本配制的早熟鲜食线椒一代杂种。始花节位为第7~8节,果实顺直,果面光滑,果肩略皱,辣味较强,青熟果亮绿色,老熟果鲜红色,果实纵径31.2cm,横径1.5cm,平均单果质量为32.6g,VC含量为672mg·kg-1,可溶性糖含量1.7g·kg-1,蛋白质含量为14.0g·kg-1,辣椒素含量为0.25g·kg-1。抗CMV、TMV,中抗疫病。一般每667m2产量3000kg左右。适合北方露地、秋延后大棚栽培,广东、广西、山西、陕西等地露地栽培。     

辣椒新品种驻椒21的选育

驻椒21是以辣椒雄性不育两用系AB0582为母本,以自交系B0318为父本选育而成的辣椒一代杂种。早熟,全生育182d(天)果实羊角形,果长19.2cm,果宽3.7cm,平均单果质量44.6g,青熟果黄绿色,每667m2产量3380kg左右,辣椒素含量26.2mg·kg-1,VC含量1040mg·kg-1。适宜早春保护地和春露地地膜覆盖栽培。     

韭菜迟眼蕈蚊在不同寄主上产卵及对LED光趋性的研究

为了实现活虫捕捉,预测田间种群发生规律,了解田间种群抗性发生动态,通过韭菜迟眼蕈蚊在4科9种不同寄主上的产卵趋性和对6种不同颜色LED光趋性测定,获得最佳寄主与光源,结合韭菜迟眼蕈蚊采集笼制备与野外田间应用,成功获得活虫。韭菜迟眼蕈蚊在马铃薯上产卵量最大,为93.56粒,占产卵总量的45.27%,且对绿色光趋性最强,诱虫量达26.75只,占全部诱虫量的40.38%。在韭菜迟眼蕈纹采集笼的野外应用中,露地捕虫量为700~800只,温室100~200只,大棚300~400只。     

Effect of rhynchophylline on TGF-β1/Smad pathway for processing ventricular remodeling in spontaneously hypertensive rats

AIM: To investigate the effect of rhynchophylline (Rhy) on blood pressure, cardiac hypertrophy and myocardial fibrosis in spontaneously hypertensive rats (SHR). METHODS: Spontaneously hypertensive rats were randomly divided into model group, high dose (10 mg·kg^-1·d^-1) and low dose (2.5 mg·kg^-1·d^-1) group of rhynchophylline, captopril group (17.5 mg·kg^-1·d^-1). Wistar-Kyoto rats were used as normal control. Respectively, systolic blood pressure was measured by tail cuff every 2 weeks. After 10 weeks, heart weight index and left ventricular weight index were calculated. The myocardial hydroxyproline and plasma angiotensin Ⅱ were detected. Moreover, basic myocardial histopathological changes and myocardial collagen fibres were observed by HE staining and Masson staining, respectively. The protein expression of TGF-β₁ and Smad3 in the myocardium was measured by the methods of immunohistochemistry and Western blot. RESULTS: Compared with SHR model group, Rhy significantly reduced blood pressure (P<0.05), the levels of HYP in the myocardium (P<0.05) and the levels of AngⅡ in the plasma (P<0.01). The pathological damages of the myocardial tissues and collagen deposition were attenuated. The protein expression of TGF-β₁ and Smad3 was significantly reduced by the treatment with Rhy (P<0.01). CONCLUSION: Rhynchophylline reduces blood pressure and adjusts to improve ventricular remodeling of SHR. The mechanism may be involved in the TGF-β₁/Smad pathway and reducing AngⅡ content.     

Effect of peroxisome proliferator-activated receptor γ agonist rosiglitazone on intestinal injury in rats undergoing orthotopic autologous liver transplantation by inhibiting inflammatory response

AIM: To investigate the effect of rosiglitazone, a peroxisome proliferators-activated receptor γ(PPARγ) agonist, on the expression of PPARγ, the activation of NF-κB and intestine injury in the rats undergoing orthotopic autologous liver transplantation(OALT).METHODS: Sprague-Dawley male rats were randomly divided into 4 groups:control group, sham group, OALT group and rosiglitazone(0.3 mg/kg, iv) pretreatment(ROS+OALT) group. The OALT model was established, and the intestinal tissues were collected 8 h after the liver reperfusion. The intestinal tissue sections were stained to visualize the damage. The expression of PPARγ and NF-κB in the tissues, the concentrations of diamine oxidase(DAO) and fatty acid-binding protein 2(FABP2) in the serum and the concentration of TNF-α and IL-6 in the tissues were measured.RESULTS: Compared with sham group, the intestinal mucosa of the rats showed obvious pathological injury after liver reperfusion in OALT group and ROS group, the Chiu,s scores of intestinal mucosa was significantly higher, and the serum concentrations of DAO and FABP2 increased(P<0.05). After rosiglitazone pretreatment, the injury of intestinal mucosa of the rats was alleviated, the Chiu,s scores was lower and the serum concentrations of DAO and FABP2 decreased(P<0.05), the PPARγ expression was obviously up-regulated in the intestinal tissues, the nuclear translocation of NF-κB was reduced and the concentrations of IL-6 and TNF-α were decreased.CONCLUSION: During perioperative period of OALT in rats, the inflammatory responses are obvious. Furthermore, obvious intestinal injury occurs. PPARγ agonist rosiglitazone obviously up-regulates PPARγ expression and inhibits the inflammation in the intestines, thus protecting against intestinal injury in rats undergoing OALT.     

适合甜菜品种鉴定的SRAP核心引物的筛选

为筛选适合甜菜品种纯度鉴定的SRAP核心引物,以12个进口国外甜菜品种为材料,对546对SRAP引物组合进行扩增,利用8%的非变性聚丙烯酰胺凝胶电泳进行分离,选择适合甜菜品种纯度鉴定的SRAP核心引物组合。结果从546对SRAP引物组合中筛选出扩增条带清晰、多态性丰富、重复性好的引物组合23对,这23对引物组合共扩增出177条多态性条带,引物的PIC值均大于0.6。平均每对引物扩增7.7条多态性条带,从理论上讲,这些核心引物完全可以实现对现有甜菜品种的鉴定。     

棒曲霉素控制技术及检测方法研究进展

系统阐述了棒曲霉素的的理化性质,分析了国内外对棒曲霉素检测、去除及降解技术研究的现状及存在问题,提出了我国目前应着力解决的关键技术性问题:①建立食品中棒曲霉素的全程控制技术体系,从源头做起,在食品生产的不同阶段嵌入关键控制技术,实现全程监测、预警及控制;②建立棒曲霉素的现场快速检测方法及监测技术体系,为实时控制提供基础数据;③进一步研究完善食品中棒曲霉素的安全去除技术方法;④研究开发食品中棒曲霉素安全的降解技术,构建降解与去除相耦合的新型工艺技术体系。     

刺山柑提取物对正常小鼠耳廓微循环的作用

研究刺山柑提取物(CSE)对小鼠耳廓微循环的影响,为改善微循环障碍性疾病提供实验依据。将正常小鼠50只随机分为5组(空白对照组,阳性对照组,CSE 1.25、2.5、5 mg/(10g·天)组。每天给药一次,连续灌胃7天。用WX-6微循环显微仪分别于末次给药后六个时间点即0.5、1.0、1.5、2.0、2.5、3.0 h于给药前耳廓相同部位测量耳廓微循环微动脉、微静脉血管口径及毛细血管开放的数目。与空白组比较,CSE低、中、高剂量组能明显增加耳廓微血管管径及毛细血管开放数目(P0.05),增强作用与作用时间及剂量成正相关,以CSE高剂量更为显著。CSE对正常小鼠微循环具有改善作用。