枯草杆菌YLNF基因编码蛋白产物性质的研究

将枯草杆菌ylnF基因经PCR扩增,酶切后插入表达质粒pET15b中,转化大肠杆菌BL21(DE3),诱导表达,金属螯合亲和层析一步纯化,酶活性测定。结果表明:ylnF基因编码产物是依赖于NAD 的前咕啉-2氧化酶,一个西罗血红素生物合成末端的酶。SDS-PAGE显示YlnF亚基分子量约22kD,全酶分子量约25kD,显示酶为单体酶。将Asp102定点突变Ala102,酶活丧失,表明Asp102在催化中起重要作用。     

西葫芦新品种春玉2号的选育

以从韩国引进的一个白皮西葫芦(代号014)材料中选出的稳定自交系007太-30-1-1为母本,以从美国引进的一个深绿色皮西葫芦(代号9505)材料中选出的稳定自交系9505青-2-3-3-6为父本配制而成早熟一代杂交种春玉2号。该品种属矮生类型,生长势强,瓜形长棒状,瓜皮浅绿色,瓜长26-30cm,瓜粗8cm左右。总产量比早青一代(CK)增产11.4%￣14.3%,一般保护地栽培产量为75000kg/hm2左右,已在陕西、甘肃、河北等地推广种植。     

双孢蘑菇同核原生质体育种技术研究：Ⅱ.同核原生质体的杂交

为建立双孢蘑菇同核原生质体杂交育种体系,本文对同核原生质体亲合性反应及杂交异核体F_1代的农艺性状和F_2代的分离与变异规律进行了研究。结果表明:双孢蘑菇同核原生质体的杂交是一个较复杂的性亲合与核迁移过程,其杂交率较低。在按气生型×气生型、气生型×匍匐型、匍匐型×匍匐型组合的520个杂交配对中,共获得113个杂交异核体,平均杂交率为22%。本研究同时建立了以菌落形态、菌丝生长速度、羧甲基纤维素酶相对活性以及酯酶同功酶电泳为手段的杂交异核体鉴定体系。     

强化木地板耐磨性能及国产化耐磨纸的研究

对强化木地板的耐磨性能和国产化耐磨纸进行了研究,重点考察耐磨纸的生产方式和热压工艺过程对产品耐磨转数和磨耗量的影响。结果表明Al2O3添加量和添加方式对产品的耐磨性能有很大影响,新研制的B型耐磨纸和进口耐磨纸有相似的磨损曲线,其耐磨转数与Al2O3添加量之间呈线性正相关,耐磨转数的增加主要表现在延长第二磨损区域,即提高初始磨损的IP值。热压工艺过程与产品的耐磨性能之间无明确关系。     

Characterization of the goldfish fecal microflora by the fluorescent in situ hybridization method

ABSTRACT:   Bacterial populations in goldfish feces were characterized by the fluorescent in situ hybridization (FISH) method. A total of nine different group-specific rRNA-targeted oligonucleotide probes were used. Approximately half of the microbial cells (57.8 ± 16.7%) were detected with a probe EUB338 and found to be bacteria. The microbial cells in 33–35 of the 35 samples from five specimens strongly hybridized with probes ALF1b, BET42a and GAM42a, suggesting that goldfish intestinal bacteria are mainly composed of α, β and γ-subclasses of Proteobacteria. The fact that a probe AER66 reacted with 25.6 ± 14.2% of the total microbial cells in all 35 samples, demonstrated that genus Aeromonas was the dominant species in the goldfish intestines. Genus Bacteroides including Bacteroides type A detected with a probe BAC303 was observed in 15 of 35 samples while other taxonomic groups determined with HGC69a, CF39a and P72 were detected in 6–11 of 35 samples. These results strongly suggest that Bacteroides shows the greatest daily fluctuation and interindividual variation in the intestines of goldfish. Moreover, the FISH method was proven to be useful for rapid enumeration of taxonomic groups of fish intestinal bacteria.     

企业投资人机智能化决策系统的设计

本文简要介绍了企业投资人机智能化决策系统的框架,着重讨论了人机智能化决策系统的特征及其与智能决策支持系统(IDSS)的区别,对人机智能化决策系统的结构与进化问题也进行了分析说明。     

断奶仔猪多系统衰竭综合症发病机理之研究进展

断奶仔猪多系统衰竭综合症(PMWS)是由2型圆环病毒(PCV2)引起猪的一种疾病。PMWS的主要病理特征是淋巴细胞衰竭。目前认为PMWS的发病机理与免疫系统有关。在此,就目前关于PCV2-PMWS-免疫系统三者之间关系的研究进展和未来主要的研究方向作一综述。     

有机质配施对日光温室CO2 浓度及番茄生理的影响

 研究了有机质配施对温室内CO₂ 浓度变化以及番茄的生理效应的影响。结果表明, 稻草和膨化鸡粪按适当比例配合撒施处理可以提高叶绿素含量, 增强光合, 抑制呼吸, 加快羧化, 从而显著提高产量。同时证实了由于稻草的持续分解, 使得温室内的CO₂ 在生育后期仍然能保持较高浓度, 可以满足长节栽培条件下番茄生长发育对CO₂ 的需求。     

Effect of CO_2 on Flammulina velutipes Fruit Body Development, and Control of CO_2 Levels During Mushroom Cultivation in Refrigerated Warehouses

Depressed fruit sales in recent years have leftunused many pear refrigeration warehouses(PRWs)located in traditional pear-producingareas of Hebei Province such as Zhao County,Ningjin and Jingzhou.As a consequence,severalproducers have commenced cultivatingFlammulina velutipesin PRWs in order to providethis mushroom during the off-season.However,the environment insidethe PRWs resultedinloweryields and poor quality mushrooms.Amongvarious environmental factors such as temperature,illuminati…     

家畜胚胎干细胞的分离培养和鉴定

家畜胚胎干细胞的分离培养和鉴定工作对于研究和利用家畜胚胎干细胞具有重大意义。本文对文献中报道的各种分离、培养和鉴定方法进行了综述 ,并介绍了几种主要家畜的胚胎干细胞在近十余年间的研究进展情况。