扁豆栽培技术

扁豆又名鹊豆,原产印度和爪哇,我国自古已有栽培。扁豆为一年生草本植物,种子适宜发芽温度为22-23℃,植株能耐35℃左右高温。根系发达强大、耐旱力强,对土壤适应性广,在排水良好而肥沃的沙质土壤或壤土种植能显著增产,是调节蔬菜伏缺较好的花色品种。     

辣椒分子连锁遗传图谱的构建及抗疫病QTL定位

以抗疫病材料perennial和感疫病材料83-60杂交得到的F2群体144个单株为试材,利用AFLP、RAPD、SSR等分子标记,采用JoinMap3.0构建辣椒分子连锁遗传图谱,该图谱包括12个连锁群,70个标记位点,其中AFLP标记54个,RAPD标记15个,SSR标记1个,覆盖长度为429.02cM,平均图距为6.13cM,连锁群长度在4.30～85.85cM之间。对F2群体进行辣椒疫病的室内人工接种,利用Windows QTL Cartographer2.0软件进行QTL分析,在第4连锁群上检测到两个QTL,解释表型差异的贡献率分别为9.5%和16.4%。     

奶牛重要经济性状的QTL作图

对奶牛产奶性能的５个性状以及２个重要的次级性状ＱＴＬ作图的方法和进展进行了综述,并讨论了奶牛ＱＴＬ定位中存在的问题。     

促紫藤盆景连年开花

紫藤根系发达。性喜阳光但也耐荫，其耐寒也耐旱，不择土壤，若露地栽培，管理简便。然而，一旦入盆养护，却不那么容易，故有“盆栽紫藤不开花”之说法。若在栽培养护中能做到以下几点，会枝叶茂盛，连年开花。     

洋石楠的四季管理

洋石楠又名石楠杜鹃，系原产于我国的高山杜鹃与欧美的杜鹃品种杂交培育的人工杂交种之一。喜凉爽湿润的半阴环境，怕酷热和烈日暴晒，稍耐寒，不耐旱，怕积水。生长适温15℃～25℃。     

柴达木双峰驼简况及其资源保护与利用浅议

柴达木双峰驼是一个体大,善驮、挽,耐寒、耐旱、耐粗饲,集产绒、产毛、产乳、产肉及役用等多种经济形状于一体的优良地方品种.近年来,由于骆驼的生产周期长,役用范围缩小等原因,数量大幅下降,平均每年减少870峰.保种工作刻不容缓.     

The QTL analysis of seedling cold tolerance in a double haploid population derived from anther culture of indica／japonica

A double haploid population,derived from an- ther culture of F_1 of intersubspecies crossZheyeqing 8/Jingxi 17(ZYQ 8/JX 17)wasused to investigate the seedling cold tolerance(SCT)in growth cabinet.By dynamically ana-lyzing every day's survival percentages of the parents and DH lines under 4-d cold tempera- ture(6-10℃)plus 9-d normal temperature(25℃)conditions,we mapped the quantitativetrait loci(QTLs)for SCT based on a molecularlinkage map constructed from this population.Results showed that two parents had signifi-     

大葱高产栽培技术

大葱适应性广，耐旱能力强，市场需求量大，只要栽培管理到位，很容易夺得高产。     

黄瓜叶面积遗传及QTL定位分析

选用2个叶面积大的黄瓜品种（D9320、D2005）和2个叶面积小的黄瓜品种（D0455、D0401）配制杂交组合，筛选叶面积差异较大的亲本组合D0401×D9320构建F2分离群体，采用加性－显性（AD）模型进行遗传分析。结果表明：黄瓜叶面积是由多基因控制的数量性状，以显性效应为主，存在一定的加性效应，狭义遗传力和广义遗传力较高。采用SSR分子标记构建遗传连锁图谱，利用复合区间法对黄瓜叶面积进行QTL定位。结果表明：CSWACC03、CSWCT05BZ和CSCTTT15a位于同一个连锁群上，连锁群总长度为68.3 cM；检测出1个与黄瓜叶面积有关的QTL，与最近标记的距离为3.6 cM，且变异贡献率为8.65 %。     

Bulked Segregant Analysis to Detect QTL Related to Heat Tolerance in Rice （Oryza sativa L.） Using SSR Markers

The study was undertaken to assess the genetic effect of quantitative trait loci （QTLs） conferring heat tolerance at flowering stage in rice. A population consisting of 279 F2 individuals from the cross between 996, a heat tolerant cultivar and 4628, a heat-sensitive cultivar, was analyzed for their segregation pattern of the difference of seed set rate under optimal temperature condition and high temperature condition. The difference of seed set rate under optimal temperature condition and high temperature condition showed normal distribution, indicating the polygenic control over the trait. To identify main effect of QTL for heat tolerance, the parents were surveyed with 200 primer pairs of simple sequence repeats （SSR）. The parental survey revealed 30% polymorphism between parents. In order to detect the main QTL association with heat tolerance, a strategy of combining the DNA pooling from selected segregants and genotyping was adopted. The association of putative markers identified based on DNA pooling from selected segregants was established by single marker analysis （SMA）. The results of SMA revealed that SSR markers, RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively, accounted for 17 and 3% of the total variation respectively. The heat tolerance during flowering stage in rice was controlled by multiple gene. The SSR markers, RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively, accounted for 17 and 3% of the total variation respectively. The two genetic loci, especially for RM3735 on chromosome 4, can be used in marker-assistant-selected method in heat tolerance breeding in rice.