Characterization of Leptospira infection in suckling and weaning rat pups

Rats are known to be the most important reservoirs of Leptospira spp. However, the leptospiral dose and age at which rats become resistant to Leptospira infection are not yet well elucidated. Aimed to characterize leptospirosis in rat pups, we found that suckling pups (4-, 7-, and 14-day old) are susceptible to leptospires and resistance starts from the weaning age (23-day old). Susceptibility of rat pups was also affected by the infecting dose of the organisms. Jaundice, decrease in body weight, and neurological symptoms prior to moribundity was evident in infected suckling pups. However, 23-day-old infected pups did not manifest any pathological changes and were able to survive the infection similar to adult rats. Based on these results, we propose the suckling rat pup as a novel animal model of human leptospirosis to investigate pathogenesis, development of host resistance, and the mechanisms involved in rats becoming maintenance hosts for leptospires.     

互联网专车服务对出租车行业垄断的影响

通过数学建模方法可知，由于出租车公司和政府部门追求垄断利润，出租车司机和消费者的福利减少，因此，对消费者和司机来说，出租车行业垄断确实是一种伤害.互联网专车服务以低廉的价格和优质的服务，是打破出租车行业垄断的有力工具.从模型分析揭示的结果可知：传统的出租车行业必须对司机、消费者、公司和政府间的利润分配制度进行深层次的改革.只有这样，才能建立一个和谐的出租车服务体系.     

Estimation of Crown Temperature of Winter Wheat and the Effect on Simulation of Frost Tolerance

Accurate estimation of winter wheat frost kill in cold‐temperate agricultural regions is limited by lack of data on soil temperature at wheat crown depth, which determines winter survival. We compared the ability of four models of differing complexity to predict observed soil temperature at 2 cm depth during two winter seasons (2013‐14 and 2014‐15) at Ultuna, Sweden, and at 1 cm depth at Ilseng and Ås, Norway. Predicted and observed soil temperature at 2 cm depth was then used in FROSTOL model simulations of the frost tolerance of winter wheat at Ultuna. Compared with the observed soil temperature at 2 cm depth, soil temperature was better predicted by detailed models than simpler models for both seasons at Ultuna. The LT50 (temperature at which 50 % of plants die) predictions from FROSTOL model simulations using input from the most detailed soil temperature model agreed better with LT50 FROSTOL outputs from observed soil temperature than what LT50 FROSTOL predictions using temperature from simpler models did. These results highlight the need for simpler temperature prediction tools to be further improved when used to evaluate winter wheat frost kill.     

大兴安岭生态功能区建设的区划分析及“G＋X”管理模式构建

依据价值分析理念,对大兴安岭生态功能区进行了区划分析,并构建了大兴安岭生态功能区建设的＂G＋X＂管理模式。     

城区道路景观共生模式研究——以金寨县将军大道为例

对小城镇沿河主干道的道路周边用地和道路中央分隔带进行景观设计和植物配置,将生态学中的共生机制原理运用到景观规划中,将地形、地貌、地物的自然景观,满足人类精神需要的人文景观,道路行车安全的功能景观和谐优化,初步探讨出一种共生机制下的小城镇道路景观共生模式。     

关于林业数学模型中参数的取值问题

针对国内林业期刊文献中普遍存在的数学模型参数取值不规范的问题，提出林业数学模型的参数取值，应遵循小数位统一和有效数统一的原则，而且有效数统一原则应该成为最高准则；同时还对与这两项原则有关的某些特定林业数表模型中参数的取值和估计问题进行讨论。     

山东农业科技期刊学术影响力分析与动态评估

利用动态评估模型对2008~2012年山东13种农业科技期刊的学术影响力进行分析。结果显示,2008~2012年中,13种农业类科技期刊的总被引频次和影响因子总体呈上升趋势,5年间,平均总被引频次增加了84.85%,平均影响因子增加了47.35%;平均学术影响力呈快速增长趋势,5年间增长了268%。各期刊发展不均衡,其中,《山东农业科学》和《中国烟草科学》学术影响力分别位居第一和第二,且发展态势良好。     

Design and Experiment of Slave Computer Control System for Applying Variable-rate Liquid Fertilizer

In order to increase the applying rate of liquid fertilizer and reduce environmental pollution, a slave computer control system for applying variable-rate liquid fertilizer was designed. The system used SMC as core processor and electrically controlled pressure regulator as execution component. The characteristic equation of the system was obtained by using classical control theory. Results indicated that the characteristic equation met the requirements of routh-criterion, which indicated the working process of the system was stable. Performance of the slave computer was verified via bench tests. Results demonstrated that there was no significant influence on the response from interclass error. The fertilization error was less than 0.9, and the fertilization accuracy was larger than 97%. The liquid fertilizer emitted by the fertilizing devices had no significant difference in uniformity, which met the demands of the slave computer control system for applying variable-rate liquid fertilizer.     

Comparison of dietary essential amino acid requirements determined from group‐housed versus individually‐housed juvenile bluegill,Lepomis macrochirus

Two 60‐day experiments were conducted sequentially to determine (i) lysine requirement of juvenile bluegill, Lepomis macrochirus based on the dose–response method, (ii) requirements for other essential amino acids (EAAs) using whole‐body amino acid profile and (iii) whether differences in growth rates of group‐housed versus individually‐housed bluegills lead to different lysine requirement levels because of the presence and absence, respectively, of social hierarchies. Seven, semi‐purified, experimental diets (isonitrogenous, isocaloric) were prepared to contain graded levels of digestible lysine (10–31 g kg⁻¹). Experiment‐1 involved group‐housed bluegills (approximately 27 g, n = 10 fish/chamber, 4 chambers/diet) whereas experiment‐2 involved individually‐housed bluegills (approximately 30 g, n = 1 fish/chamber, 14 chambers/diet). Fish were fed twice daily to apparent satiation. Bluegill growth responses in both experiments generally improved (P < 0.05, anova ) with increasing dietary lysine levels from 10 to 16 g kg⁻¹, and then levelled off with further increase in lysine level (P > 0.05). Optimal dietary lysine level (digestible basis) was estimated to be 15 g kg⁻¹ based on broken‐line regression analyses of relative growth rate and feed conversion ratio with no differences being observed between the two rearing methods. Determined dietary requirement levels for other EAAs ranged from 2.4 g kg⁻¹ (tryptophan) to 15.3 g kg⁻¹ (leucine).     

保健食品中原花青素测定的不确定度评定

对保健食品中原花青素的测定进行不确定度的评定,建立不确定度评定程序和方法。依据《保健食品检验与评价技术规范》建立数学模型,进行不确定度的计算并合成不确定度。找出了影响保健食品中原花青素测定的主要因素,分析归纳了不确定度分量的主要来源。评定程序和方法符合规范要求,适用于同类型试验不确定度评定。