增施氮肥、硼肥对花期遮阴条件下玉米籽粒产量的影响

遮阴已成为影响夏玉米产量的重要因子，提高玉米耐阴性可为高产、抗逆栽培提供理论依据。以‘苏玉29’为试验材料，系统研究了小喇叭口期增施氮肥、硼肥缓解花期遮阴胁迫对夏玉米形态建成、物质积累和产量的影响。结果表明：遮阴处理降低了株高、茎粗、单株叶面积和干物质积累量，并延长了散粉吐丝间隔期。与遮阴处理比，小喇叭口期增施硼肥、氮肥不同程度提高了株高、茎粗、单株叶面积和干物质积累量，且同时增施硼肥、氮肥增幅最大。遮阴处理显著增加了秃尖长，降低了穗粒数和粒重，增施硼肥氮肥缓解了遮阴对突尖长、穗粒数和粒重的影响。增施硼肥、氮肥和同时增施硼肥、氮肥处理产量分别较遮阴处理提高36.7%、8.0%和43.1%。遮阴处理降低叶面积影响干物质积累，产量的降低与突尖增长、穗粒数较少和粒重降低有关。小喇叭口期增施硼肥和氮肥是缓解花期遮阴对产量不利影响的有效措施。 关键词：玉米；遮阴；氮肥；硼肥；产量；农艺性状     

基于扩散理论的华北春玉米生理成熟后籽粒脱水过程分析

玉米机收籽粒可以显著提高玉米的生产效率,是玉米生产的发展方向。生理成熟后的籽粒含水率是决定机收质量的关键,受品种、密度和气候等多种因素影响。准确估算生理成熟后玉米籽粒含水率,进而分析其主要影响因素,最终确定玉米收获时间和筛选适宜机收的品种,对玉米主产区华北的春玉米籽粒机收发展具有重要意义。因此,于2017年和2018年在河北省泊头、南大港、玉田和山西榆次进行了两年田间春玉米试验,每年设置7个共性品种,每个品种3个密度,对生理成熟后籽粒含水率、品种性状、气象和管理要素进行了监测,并利用基于扩散理论考虑空气温湿度的脱水模型对籽粒含水率进行了模拟,进而计算脱水曲线下的面积(AUDDC),用以筛选脱水优异的玉米品种。结果表明,基于扩散理论的籽粒脱水模型对玉米生理成熟后籽粒含水率的模拟效果较好;年份、地点和品种对生理成熟时籽粒含水率(M_0)和水分扩散速率(k)具有显著影响,密度对脱水参数影响不显著。逐步线性回归分析得到灌浆期参考作物蒸发蒸腾量(ET_0)、最高气温和灌水量对M_0具有显著的正效应,生理成熟后30 d内ET0和灌浆中后期降雨对k具有显著的正效应,全生育期降雨对k具有显著的负效应。品种性状中对M_0影响最大的为苞叶层数(正效应),对k影响最大的为叶片数(负效应)。通过模型计算得到,生理成熟后10d华北地区春玉米籽粒含水率可以下降到28%,籽粒含水率下降到25%的概率为50%。由模型计算得到各品种生理成熟后10 d内的AUDDC,与AUDDC平均值比较发现‘京农科728’‘张1453’‘华农887’‘广德5’和‘金科玉3306’为脱水表现优异的品种。     

多效唑对杂交中稻不同密肥群体产量和抗倒伏性的影响

为探究高产稻田中防止倒伏发生的早期诊断技术,2015-2016年,以大面积推广的杂交中稻高产新品种蓉18优1015为试验材料,设计3因素完全因子试验,研究不同密肥群体下喷施多效唑对稻谷产量和抗倒伏性的影响。结果表明,随着施氮量和移栽密度的增加,稻谷产量提高,植株抗倒力下降,以施氮量150 kg·hm^-2和移栽密度18.75 万穴·hm^-2的产量较高。不同密肥群体下施用多效唑对产量和植株抗倒伏性有显著影响,施用多效唑后植株抗倒力增强,但产量因穗粒数下降而减少。不同密肥处理下施用多效唑对产量影响各异。于水稻最高苗期施用多效唑使植株重心高度、弯曲力矩、倒伏指数显著降低,折断弯矩则明显提高,穗粒数平均减少5.24~7.87粒。多效唑对产量的影响表现为低施氮量下因穗粒数减少而减产;中施氮量下产量差异不显著;高施氮量下植株未倒伏、籽粒灌浆结实正常,因结实率和千粒重高而增产。综上所述,肥力水平和施氮量高的稻田于最高苗期施用多效唑有利于水稻产量提高和控制后期倒伏。本研究为指导大面积水稻高产稳产提供了科学依据。     

Application of urease inhibitor improves protein composition and bread‐baking quality of urea fertilized winter wheat

Background: Nitrogen losses is an economic problem for wheat production and a high risk to the environment. Therefore, improved N fertilizer management is a key to increasing the N efficiency and minimizing N losses. To increase N efficiency, enhanced fertilizers such as urea combined with urease inhibitor can be used. Aims: The aim of present study was to evaluate the effects of different N forms on grain storage protein subunits in winter wheat and to examine whether the observed changes correlate with parameters of baking quality. Methods: The investigation was performed over two consecutive years at two locations in Germany. Protein subunits were analyzed by SDS‐PAGE. Results: Protein concentrations were similarly increased after fertilization with ammonium nitrate and urea + urease inhibitor. Analysis of the individual storage protein fractions indicated that both fertilizers specifically enhanced ω‐gliadins and HMW glutenins, but the effect was more pronounced in the ammonium nitrate treatment. Application of urea + urease inhibitor had greater influence on the protein composition and resulted in higher specific baking volume as well as the best fresh keeping ability, in comparison with urea treatment. Conclusion: Considering that the urea + urease inhibitor treatment resulted in almost comparable improvements of NUE and baking quality, with the additional benefit of reduced N losses in combination with easy handling, urea + urease inhibitor can be recommended as a viable alternative to both urea alone and ammonium nitrate treatments. This opens up an opportunity for the reduction of N loss in wheat production when use of urea is preferred.     

122份栽培桃品种（系）黄白肉性状的分子标记辅助鉴定

【目的】 类胡萝卜素裂解双脱氧酶基因（Carotenoid Cleavage Dioxygenases 4,CCD4）控制桃果肉颜色（白/黄）,CCD4存在3种等位基因。本研究利用Indel、SSR荧光标记毛细管电泳及SNP鉴定等基因分型技术分析我国主要桃黄白肉品种（系）中CCD4等位基因的差异,为主要黄/白肉品种（系）的基因型鉴定、亲本选配和选择相应的分子标记对不同来源子代的果肉颜色进行鉴定奠定基础。【方法】利用已经报道的桃不同果肉颜色中CCD4等位基因3种突变类型,合成不同引物进行PCR扩增,LTR反转录转座子插入突变经1%的琼脂糖凝胶电泳检测,CT单元重复的PCR产物在ABI3730XL测序仪上进行SSR荧光标记毛细管电泳检测,SNP标记经Sanger测序后利用ContigExpress软件分析CCD4等位基因的碱基替换（A→T）。综合以上结果,统计每份材料中CCD4等位基因的突变类型与果肉颜色的一致性。【结果】通过对不同来源的122份桃品种（系）材料进行基因型分析,发现CCD4发生LTR反转录转座子插入突变材料的基因型共有31份,占总材料的25.4%,其中纯合插入突变材料的片段扩增长度为729 bp,共有8份,占总突变的25.8%;CCD4发生微卫星重复序列突变材料存在2 bp的插入,扩增片段长度为179 bp,该类型共有68份,占总材料的55.7%,其中纯合插入材料25份,占总突变的36.8%;CCD4发生A→T碱基替换突变的材料较少,仅有1份,占总材料的0.82%,实际应用中可以不考虑该种类型。CT和LTR插入的两种突变类型的黄肉品种（系）有7份,占总材料的5.7%。研究结果表明,LTR反转录转座子插入突变和微卫星序列重复突变是黄肉桃中CCD4等位基因的主要突变类型。其中CCD4发生一种纯合突变或两种杂合突变桃品种（系）为黄肉类型,分子标记鉴定结果与调查的122份桃品种（系）黄白肉表型性状完全一致,准确率为100%。【结论】采用分子标记明确了122个桃品种（系）黄/白肉性状的基因型,为不同亲本组合子代表型鉴定的标记类型选择提供了技术支撑,为建立桃种质材料黄/白性状的分子辅助育种体系和黄肉桃的选育奠定了基础。     

The use of remote sensing to detect the consequences of erosion in gypsiferous soils

Tillage practices on sloping ground often result in unsustainable soil losses impairing soil functions such as crop productivity, water and nutrients storage, and soil organic carbon (SOC) sequestration. A sloping olive grove (10%) was planted in shallow gypsiferous soils in 2004. It was managed by minimum tillage; the most frequent management practice in central Spain. The consequences of erosion were studied in soil samples (at 0–10, 10–20, and 20–30 cm depths) by analyzing SOC, available water and gypsum content, and by detecting spectral signatures using an ASD FieldSpecPro® VIS/NIR-spectroradiometer. The Brightness index (BI), Shape index (FI), and Normalized Difference Vegetation Index (NDVI) were derived from the ASD spectral signatures and from remote sensing (Sentinel-2 image) data. The development of these young olive trees was estimated from the measured diameter of the trunks (17 ± 18 cm diameter). In 20–30 cm of the soil, the carbon stock (38 ± 18 Mg ha⁻¹) as well as the available water content (12 ± 6%) was scarce, affecting the productivity of the olive grove. The above-mentioned indices obtained from the laboratory samples and the pixels of the Sentinel-2 image were significantly (p < 0.01) correlated, with a correlation coefficient of around 0.4. The BI was related to the gypsum content and the slope of the plot. The FI was related to the carbon and water contents. The NDVI derived from the satellite image identified the influence of soil degradation on the trees and the carbon content. The spatial-temporal changes of the indices might help in tracking soil changes over time.     

Maize harvest index and water use efficiency can be improved by inhibition of gibberellin biosynthesis

Possibilities to improve maize harvest index and nutrient utilization efficiency by application of plant growth regulators were investigated. In container experiments, the effects of different growth regulators on the development of the maize (Zea mays L.) cultivars Pioneer 3906 and Fabregas were tested. Paclobutrazol (PAC) and chlorocholine chloride (CCC), two inhibitors of gibberellin biosynthesis, as well as gibberellic acid (GA₃) were applied at growth stage V5. Three weeks after application of PAC, shoot growth of both maize cultivars was strongly affected with a significant decrease in plant height in the PAC treatment by 44% and 36% for Pioneer 3906 and Fabregas, respectively. The growth‐retarded plants had higher leaf areas and reduced transpiration rates. The higher shoot growth after GA₃ application was accompanied by a reduction in leaf area and an increase in transpiration rate during 1 week before anthesis. CCC treatment showed no significant effects on plant height, leaf area and transpiration rate. The PAC‐treated cultivar Pioneer 3906 produced several cobs per plant, which were mainly barren at maturity. However, PAC application to Fabregas resulted in just one cob per plant with good kernel development and a grain yield, which was not significantly reduced in comparison with the control. With this similar grain yield in combination with a straw yield decrease of 32%, the harvest index was significantly improved by 12%. In addition, with PAC‐treated Fabregas plants, a 19% increased water use efficiency of the grain (WUE_grain) during the critical period of kernel setting was achieved. In this maize cultivar, CCC application also improved harvest index by 5%, but no effect on WUE_grain occurred. GA₃ treatment decreased harvest index of both maize cultivars, and it either reduced WUE_grain (Pioneer 3906) or showed no effect (Fabregas). Utilization efficiencies of N, P and K were not increased with growth regulator application, even in the PAC‐treated Fabregas plants with a significantly improved allocation of assimilates to the grain, mirrored by the higher harvest index. The results indicate that fertilizer applications must be adjusted to the reduced demand of growth‐retarded plants, most likely leading to higher nutrient utilization efficiencies.     

相变材料在绿色储粮中的应用探讨

相变材料能够储存或释放显热,在熔化或凝固过程中虽然温度不变但吸收的潜热却相当大。根据相变材料可以吸收环境热能并在需要时向环境发出热能的特征,研究相变材料在粮食仓储中的应用,减小外界环境对粮仓温度的影响,达到恒温绿色生态储粮的目的。     

储粮害虫磷化氢抗性分子遗传学研究进展

由于长期单一使用磷化氢,储粮害虫对其产生的抗药性日益增强,已成为威胁粮食安全储藏的重大问题。自从发现两个磷化氢抗性基因rph1和rph2,以及完成赤拟谷盗全基因测序以来,储粮害虫磷化氢抗性基因的相关研究迅速发展。近年来,在抗性基因的鉴定、表达、进化、突变等研究方面取得了一定成果,为储粮害虫磷化氢抗性治理提供了新的科学指导。本文就储粮害虫磷化氢抗性分子遗传学的研究进展进行综述。