利用RIL群体和自然群体检测与花生含油量相关的SSR标记

以远杂9102×中花5号杂交后代衍生的重组近交系F₈代家系为材料，在含油量测试的基础上，选用10份低油材料(平均含油量52.91%)、12份高油材料(平均含油量58.85%)以及亲本进行SSR引物筛选，通过631对SSR引物扩增，筛选出来源于7对引物的13个有显著差异的片段可以有效区分低油材料和高油材料。以这7对差异引物在F₈ RIL群体中扩增，对20份低油家系材料(含油量<55%)和45份高油家系材料(含油量>56%)进行统计分析，获得1个与花生含油量相关的分子标记2A5-250/240，其中，标记2A5-250为低油材料(含油量<55%)所拥有，相符率为95.0%，标记2A5-240为高油材料(含油量>56%)所拥有，相符率为88.9%。用SSR标记2A5-250/240检测11份高油(平均含油量为55.93%)栽培种花生和11份低油(平均含油量为48.41%)栽培种花生，结果表明，标记2A5-240与高油栽培种花生的符合率为63.6%，2A5-250与低油栽培种花生的符合率为90.9%。在19份高油(平均含油量为58.60%)野生花生中，10份野生花生能检测到标记2A5-240。综合分析RIL群体和自然群体的研究结果表明，标记2A5-250/240可用于花生含油量分子标记辅助选择。     

Identification of differentially expressed genes associated with bud dormancy release in tree peony （Paeonia suffruticosa） by suppression subtractive hybridization

A subtractive cDNA library was developed to study genes associated with bud dormancy release in tree peonies.In order to identify genes that are highly expressed in buds released from dormancy,588 clones were examined by differential screening.Of these,185 clones were selected to be sequenced.A total of 37 unique sequences were obtained of which only 31 sequences have matches in the NCBI database or the Arabidopsis thaliana protein database.Semi-quantitative RT-PCR was used to confirm further the expression...     

Oil Palm — Achievements and Potential

Abstract

Cultivation of the oil palm (Elaeis guineensis Jacq.) has expanded tremendously in recent years such that it is now second only to soybean as a major source of the world supply of oils and fats. Presently, Southeast Asia is the dominant region of production with Malaysia being the leading producer and exporter of palm oil. This paper reviews the various factors that have led to oil palm occupying its present position, including biological, technical, managerial, environmental, and socio-political aspects. Biological features recognised as critical to the high productivity of the crop are examined. These include its perennial and evergreen nature (giving a continuous year-round canopy cover intercepting a high proportion of incoming radiation), the year-round production of fruit bunches and the high partition of total assimilates into harvested product. Scientific and managerial aspects contributing to the success of the crop include the significant genetic improvements and production of high quality planting materials, the development and application of finely-tuned agronomic practices, the appropriate scale and efficient organisation of oil palm plantations and the continuous R&D and good infra-structural support provided in the main producing countries. The programmes of crop improvement through the utilisation of traditional breeding and selection methods, the development and benefits of vegetative propagation techniques using tissue culture and ongoing efforts to apply molecular and genetic engineering techniques to improve and modify oil composition, are reviewed. Finally, the nutritional qualities of palm oil as a healthy component of diet are briefly described.     

PASSAT领驭2.0自动变速箱偶尔出现紧急运行状况检修分析

结合PASSAT领驭2.0轿车偶尔出现自动变速箱Ⅲ挡运行的紧急运行状况故障,分析了故障码、有关传感器电路,以及故障产生的原因,据此找出了故障点,并排除了故障。     

牡丹抑制栽培技术要领

通过对抑制栽培中苗木品质、储藏条件、温控设施、温度、湿度、光照等条件的论述，总结出牡丹抑制栽培过程中需注意的各种技术要领。     

Organic acid content and composition of the olive fruits during ripening and its relationship with oil and sugar

In this work, the contents of oil, sugar and organic acids of olive fruit samples (Domat, Memecik and Uslu) were determined by using HPLC at different maturation stages. Olive fruit samples presented a common organic acid profile, composed of four constituents: oxalic, citric, malic and succunic acids. Total organic acid content of Domat variety was found to be 4787.1 ± 4.53 mg/100 g in January. Memecik variety had the highest value at the beginning of the ripening period (6385.08 ± 4.90 mg/100 g). The amount of organic acid in Uslu variety reached up to the value of 10942.5 ± 30.41 mg/100 g. The results were evaluated statistically to determine relationship between the oil, sugar and organic acid content in three olive varieties during maturation. A positive correlation was found between the total sugar and organic acid (r = 0.54, p < 0.01) whereas a low positive correlation was observed between the contents of oil and total organic acids (r = 0.46, p < 0.05) in olive varieties.     

禽霍乱油乳剂灭活苗（TJ株）的研制与应用

从天津地区某些发病鸡场分离的２０余株禽多杀性巴氏杆菌中筛选４株（ＴＪ株）作为制苗用菌种，制成油乳剂灭活苗，进行物理性状检验与安全性，免疫性试验，用实验室制备的禽霍乱油乳剂灭活苗（ＴＪ株）对大港，静海等发病鸡场进行免疫接种，完全控制了禽霍乱的流行。     

木材干燥窑的保温性与节能分析

常规砖砌壳体木材干燥窑热能消耗大，节能潜力很大，本文通过对窑体保温材料和大门密，保温性能改进措施的分析及应用，结果表明，壳体热损失减少６０％以上，总能源可减少１２％以上，节能效果显著，此外对用热油作为导热介质，也探讨了其节能的可行性。     

Oil separation from wet-milled corn germ dispersions by aqueous oil extraction and aqueous enzymatic oil extraction

The many recent dry-grind plants that convert corn to ethanol are a potential source of substantial amounts of corn oil, if an economical method of separating it can be developed. Oil separation from corn germ by aqueous extraction (AE) was studied. Batches of 5–20% germ in a buffer solution were preheated in a pressure cooker, ground in a blender or colloid mill, churned in an incubator/shaker and centrifuged to separate a free oil fraction. The highest oil yields were obtained when the germ mass fraction was between 0.1 and 0.16. Addition of an enzyme solution to the ground germ suspension prior to churning, aqueous enzymatic extraction (AEE) gave an oil yield twice as high as the AE yield, carried out using similar conditions. Both types of extraction produced the most oil after a 122 °C cook followed by churning at 160 rpm and 70 °C. Oil yield increased linearly with dispersion loading, from 0.5 up to 1.0 g/cm² based on beaker cross-section (0.09–0.12 germ mass fraction). Over a several month period the germ used for the extractions became more difficult to extract, despite storage at 4 °C.     

牡丹切花保鲜研究进展

牡丹是我国的传统名花之一,牡丹切花在国际市场有一定的需求。笔者对有关牡丹切花保鲜研究概况和研究方法进行了系统的论述,并对牡丹切花保鲜研究中存在的问题和研究重点进行了展望。