蜜糖文心兰的组织培养与工厂化生产技术创新

介绍了蜜糖文心兰的组织培养体系与工厂化生产技术,指出用花梗和侧芽作外植体进行组织培养,可从再生芽中诱导出类原球茎,再通过芽和类原球茎2种途径进行增殖.在培养中观察发现,发育粗壮但未分化假鳞茎的再生芽诱导类原球茎能力最强,发育程度不同的类原球茎增殖特性不一,愈伤态类原球茎具较强增殖能力,增殖率最高;通过液体振荡培养7 d可以促进增殖潜能的形成,淘汰发育不良褐化死亡的类原球茎,使类原球茎发育同步化,通过悬浮培养后类原球茎出苗整齐一致,提出了液体悬浮培养和固体培养结合的高效工厂化生产新程序.     

Studies on Genetic Transformatiom of Embryogenic Suspension Cultures of Sweetpotato

Genetic transformation of embryogenic suspension cultures of sweetpotato cv. Lizixiang wasconducted by using Agrobacterium tumefaciens strain A208SE harboring the binary vectors pROA93 with β-glucronidase (GUS) and neomycin phosphotransferase (NPT Ⅱ ) genes. The results indicated that embryogenicsuspension cultures precultured for 1 -3 d were suitable for the transformation. The optimal cocultivation timewas 4 - 5 d. The optimal concentration of kanamycin was 50-75 mg L-1 for suspension culture and 100 mg L-1for embryogenic callus proliferation and plant regeneration. The optimal concentration of carbencillin was 100mg L-1. Transgenic plants identified with GUS assays and PCR analyses were obtained.     

悬栅消能率的投影寻踪回归数值模拟及检验

应用投影寻踪回归技术，对非正态、非线性悬栅消能率实验数据，用1／5数据建模拟合，4／5数据留做预留检验，拟合合格率92％，预留检验合格率92％，并与激光测速得出的消能率及原型观测的消能率完全吻合。     

植物细胞生长周期中内外源细胞激动素的变化

应用细胞同步培养技术、高压液相色谱分析（ＨＰＬＣ）和酶偶联免疫法（ＥＬＩＳＡ）分析了胡萝卜细胞在悬浮培养时生长分裂周期的变化并对内源激动素类物质（玉米素Ｚ－Ｚｅａｔｉｎ、核糖基玉米素ＺＲ－ｚｅａｔｉｎｒｉｂｏｓｉｄｅ、二氢玉米素ＤＨＺ－ｄｉｈｙｄｒｏｚｅａｔｉｎ、核糖二氢玉米素ＤＨＺＲ－ｄｉｈｙｄｒｏｚｅａｔｉｎｒｉｂｏｓｉｄｅ、异戊烯基核糖基腺嘌呤ＩＰ－ｉｓｏｐｅｎｔｅｎｙｌａｄｅｎｉｎｅ和核糖基异戊烯基腺苷ＩＰＲ－ｉｓｏｐｅｎｔｅｎｙｌａｄｅｎｉｎｅｒｉｂｏｓｉｄｅ）含量进行定量分析．从用荧光显微技术分析ＤＮＡ含量变化结果看出，附加有激动素０．１×１０－６·Ｌ－１的悬浮液中，胡萝卜细胞的分裂周期为８．５ｈ，比不加外源激素的处理短１ｈ．在无外源激素存在的情况下，细胞分裂期中内源细胞分裂素类物质除ＺＲ之外，其余的Ｚ、ＤＨＺ、ＤＨＺＲ、ＩＰ和ＩＰＲ的含量在分裂的第６．５ｈ均有一个峰值出现，ＺＲ在第４．５和８．５ｈ有峰值出现．外加细胞分裂素的处理中．Ｚ、ｉＰ和ｉＰＲ整个周期无明显含量变化，ＤＨＺ和ＤＨＺＲ在第６．５ｈ出现峰值．ＺＲ在第８．５ｈ时也出现峰值．说明附加细胞激动素可协调Ｚ．ｉＰ和ｉＰＲ的积累，加速利用?     

8自由度乘坐动力学模型及时域仿真

为克服频率响应法对车辆平顺性预测的局限性，建立了8自由度的汽车乘坐动力学模型和随机路面激励的时域模型。乘坐动力学模型考虑了包括坐椅在内的车辆的垂直、俯仰和侧倾3种运动。利用MATLAB／SIMULINK仿真工具对某型车辆在B级路面行驶时的平顺性进行了时域仿真分析。研究结果表明，此方法能够快速地对车辆在随机路面行驶的平顺性进行预测和评价。     

恩诺沙星混悬液在猪体内残留消除规律研究

采用高效液相色谱法研究恩诺沙星(口服)混悬液在猪体内各组织中的残留消除规律.恩诺沙星(口服)混悬液,按每头猪10 mg/kg体重的剂量灌服给药,连续使用3 d之后,宰杀猪,取组织.组织样品经磷酸盐缓冲液提取,C18固相萃取柱净化,过膜,用流动相0.05 mol/L磷酸溶液/三乙胺一乙腈(82+18)溶解,微孔过滤,进行...     

悬液芯片系统在检测领域的研究进展

悬液芯片系统诞生于20世纪90年代中期,由美国Luminex公司的研制。该系统使用荧光编码的聚苯乙烯微球作为特异性反应的固相载体,通过偶联试剂的作用,将蛋白质、寡核苷酸、小分子肽类及脂肪偶联到微球的表面构成不同的检测探针。在反应体系中,检测探针通过特异性反应（如抗原―抗体,配体―受体,核酸互补碱基对）捕获与探针相对应的分析物,用荧光标记物标记与探针结合的分析物得到悬液芯片系统的检测物。依据微球内部红色和橙色荧光染料比例的不同,将供悬液芯片系统使用的聚苯乙烯微球分为100种不同的型号。检测器对荧光标记物荧光强度进行检测的同时能分辨不同型号的微球,并将不同型号微球对应的标记物的荧光强度进行分别记录,最终实现一次分析多种检测物的目的。作者综述了悬液芯片系统的原理及在蛋白质、核酸检测领域的研究进展。     

苹果体细胞悬浮培养及植株再生研究

以苹果试管苗叶片的再生不定芽嫩叶为试材,对苹果体细胞悬浮系的建立及植株再生的影响因素进行了研究.结果表明:在MS+NAA 0.5 mg/L+BA 2.0 mg/L培养基上可诱导获得高活力的愈伤组织.将该愈伤组织转入MS+2,4-D 2.0 mg/L+BA 1.0 mg/L液体培养基中培养,采用无菌筛网分离获得含单个细胞和少于8～10个细胞的小细胞团进行继代培养,建立体悬浮细胞培养系.将悬浮细胞转到MS+2,4-D 2.0 mg/L+BA 1.0 mg/L的固体增殖培养基上暗培养25 d后,可形成微型愈伤组织,将该愈伤组织转到MS+BA 5.0 mg/L+IAA 0.5 mg/L的植株再生培养基上暗培养30 d后转入光下,光照培养30 d后53％的愈伤组织可再生植株.该研究建立的苹果体细胞悬浮培养技术,不仅可用于细胞融合及遗传转化过程中杂种细胞和转化细胞的分离及植株再生研究,而且对于利用组织培养技术筛选变异株系也具有重要意义.     

滑柱摆臂式悬架车辆之转向梯形断开点的确定

根据机构学原理,将目前农用运输车采用的滑柱摆臂式悬架等效成铰接四杆机构;通过实例计算和断开点主要影响因素分析,提出了正确选择转向梯形断开点位置的新方法。     

Soil salinity and its distribution determined by soil sampling and electromagnetic techniques

Abstract. Diagnosis of soil salinity and its spatial variability is required to establish control measures in irrigated agriculture. This article shows the usefulness of electromagnetic (EM) and soil sampling techniques to map salinity. We analysed the salinity of a 1‐ha plot of surface‐irrigated olive plantation in Aragon, NE Spain, by measuring the electrical conductivity of the saturation extract (EC_e) of soil samples taken at 22 points, and by reading the Geonics EM38 sensor at 141 points in the horizontal (EM_H) and vertical (EM_V) dipole positions. EM_H and EM_V values had asymmetrical bimodal distributions, with most readings in the non‐saline range and a sharp transition to relatively high readings. Most salinity profiles were uniform (i.e. EM_H=EM_V), except in areas with high salinity and concurrent shallow water tables, where the profiles were inverted as shown by EM_H > EM_V, and by EC_e being greater in shallow than in deeper layers. The regressions of EC_e on EM readings predicted EC_e with R² > 84% for the 0–100 to 0–150 cm soil depths. We then produced salinity contour maps from the 141 EC_e values estimated from the electromagnetic readings and the 22 measured values of EC_e. Owing to the high soil sampling density, the maps were similar (i.e. mean surface‐weighted EC_e values between 3.9 dS m^?1 and 4.2 dS m^?1), although the electromagnetically estimated EC_e improved the mapping of details. Whereas soil sampling is preferred for analysing the vertical distribution of soil salinity, the electromagnetic sensor is ideal for mapping the lateral variability of soil salinity.