基于GIS技术的长汀县森林资源分类经营管理

依据国家林业局颁发的《全国森林资源经营管理分区施策导则》所提供的森林资源分类分区指标,利用GIS空间分析技术,综合长汀县林业基本图层、遥感影像数据、基础地理数据等,对福建省长汀县的森林资源进行了有效的分区分类,将其分为严格保护类型组、重点保护类型组、保护经营类型组和集约经营类型组4个类型,对分区的结果进行分析,并提出了相应的经营管理对策,为森林资源的有效经营管理提供了决策支持。     

牡丹峰国家森林公园开发强度控制策略

通过构建景观敏感度和景观阀值评价体系,对牡丹峰国家森林公园各景区的景观敏感度和景观阀值进行评价;根据评价得到的各个景区的敏感度和阀值级别,分别制定了各个景区相应的开发利用策略。     

四种桉树青枯菌DNA提取方法及PCR检测灵敏度比较

以带有青枯菌Ralstonia solanacearum的桉树组织为材料,采用4种不同的提取方法抽提青枯菌DNA,同时利用青枯菌的特异性引物进行PCR扩增,比较了4种DNA的提取方法及PCR检测的灵敏度。结果表明,煮沸法和热裂解法操作相对简单,但检测灵敏度较低,检测限分别为104CFU/mL和103CFU/mL;简化提取法和试剂盒法检测效果较好,均可检测到102CFU/mL的青枯菌;简化提取法比试剂盒法操作更简单,检测成本低,具有更高的应用价值。     

Bryophytes as potential indicators of forest integrity

Forest integrity has been proposed as one conservation endpoint that integrates desirable characteristics such as natural biodiversity, stand structure and continuity. Although its defining criteria are still under discussion, any surrogates must effectively represent or predict their status, and be easier to measure than the criteria themselves. Bryophytes have been proposed as such surrogates, because they are important components of forest integrity, and considerable research indicates that some groups are sensitive to the changes associated with specific forest management regimes. The objectives of this paper are (1) to review the issues in determining indicators of forest integrity, including desirable qualities in such indicators, (2) to review the state of knowledge concerning bryophytes as components of forest integrity (i.e. their responses to forest management practices), and (3) to assess bryophytes as potential indicators of forest integrity, in terms of both qualities desirable in indicators and our understanding of bryophyte response patterns. Although bryophytes possess some characteristics that suggest potential indicator value, many challenges prevent their reliable application. I highlight key areas in which research is required to identify operational bryophyte indicators of forest integrity. Along with a standardized protocol to select and calibrate such indicators, we urgently require strategic research to compile data on undisturbed reference forests on which to base selection of endpoints; species-specific ecological tolerances, with consideration of complex interactions; mechanisms of response to disturbance, with consideration of temporal aspects; population viability thresholds; and recruitment effects on community assembly. Whether we succeed in finding bryophyte indicators of forest integrity, this research would also provide the data to monitor and interpret the integrity of the bryophyte community.     

林分平均胸径100倍圆法测定每公顷断面积的理论初探(之二)--误差分析与修正系数

通过对100Dg圆公式含义的分析,对影响林分断面积精度的2个主要因子:样地株数密度与林分平均胸径估测值进行了误差分析与探讨,提出了样地株数密度估计林分株数密度采用典型选样,林分平均胸径估测值采用目测法等相应解决办法。其中,当目测值有偏差时采用修正系数可使100Dg圆的可靠性进行了理论上的探讨。     

单板条双向定向重组装饰板材研制

介绍了一种单板条双向定向重组装饰木板的制造方法。采用该法制成的重组木板是由多条厚度为0.5~5mm、宽度为10~100mm、长度不限的单板条以宽面倾斜贴合并经施胶固化而成的板材;板材中单板条的长度方向与板材长度方向大致相同,单板条的宽度方向与板材的板面相交成15°~85°的夹角。该重组木板的制备工艺包括:1)单板制备;2)单板条制备;3)单板条端头加工;4)单板条干燥;5)单板条施胶;6)单板条陈化;7)双向定向组坯;8)压固成型;9)毛坯板材加工处理。采用该法制成的重组木板材表面具有类似于天然木材表面的条带状花纹,具有很好的装饰效果,可直接用于地板、家具面板和建筑装饰面材等领域。     

日本乙型脑炎病毒ELISA抗体检测试剂盒的研制及初步应用

采用日本乙型脑炎病毒单克隆抗体预包被酶标板,将纯化的日本乙型脑炎病毒(JEV)作为检测用抗原,利用包被捕获法建立了用于检测猪乙型脑炎抗体的间接ELISA法。采用建立的ELISA法对50份已知阴性血清样本检测,临界OD450nm值为0.343,ELISA与IFA对200份血清进行平行检测,总符合率为92.9%,与商品化的同类国产试剂盒的符合率为95%。与其他常见的猪病毒阳性血清抗体无交叉反应,2~8℃保存12个月稳定。研制的ELISA抗体检测试剂盒为临床JEV血清抗体检测及其疫苗的免疫效果评价提供了技术手段。     

Accounting for unknown foster dams in the genetic evaluation of embryo transfer progeny

Animals born by embryo transfer (ET) are usually not included in the genetic evaluation of beef cattle for preweaning growth if the recipient dam is unknown. This is primarily to avoid potential bias in the estimation of the unknown age of dam. We present a method that allows including records of calves with unknown age of dam. Assumptions are as follows: (i) foster cows belong to the same breed being evaluated, (ii) there is no correlation between the breeding value (BV) of the calf and the maternal BV of the recipient cow, and (iii) cows of all ages are used as recipients. We examine the issue of bias for the fixed level of unknown age of dam (AOD) and propose an estimator of the effect based on classical measurement error theory (MEM) and a Bayesian approach. Using stochastic simulation under random mating or selection, the MEM estimating equations were compared with BLUP in two situations as follows: (i) full information (FI); (ii) missing AOD information on some dams. Predictions of breeding value (PBV) from the FI situation had the smallest empirical average bias followed by PBV obtained without taking measurement error into account. In turn, MEM displayed the highest bias, although the differences were small. On the other hand, MEM showed the smallest MSEP, for either random mating or selection, followed by FI, whereas ignoring measurement error produced the largest MSEP. As a consequence from the smallest MSEP with a relatively small bias, empirical accuracies of PBV were larger for MEM than those for full information, which in turn showed larger accuracies than the situation ignoring measurement error. It is concluded that MEM equations are a useful alternative for analysing weaning weight data when recipient cows are unknown, as it mitigates the effects of bias in AOD by decreasing MSEP.     

Study on Biological Characteristics and Drug Sensitivity of Haemophilus parasuis Isolated in Sichuan Province

The study was aimed to investigate the biological characsteristics and drug sensitive tests of Haemophilus parasuis isolated in Sichuan province.This paper reported the biological characteristics,16S rRNA gene PCR and drug sensitive test of the isolates by technique of microbiological and molecular biology.The results showed that the isolates had micro anaerobic and could produce satellite phenomenon,the isolates were just same in biochemical characteristics with field isolates in other area.All the isolates amplified 821 bp 16S rRNA gene.And drug sensitive tests showed that the isolates were highly sensitive to cefradine,ampicillin,cefradine,amoxicillin,cefaclor,ofloxacin,cefotaxime and furadantin,the sensitive rate was 75.61% to 87.80%,but the isolates were high resistance to lincomycin,carbenicillin,enrofloxacin,amikacin and sulfamethoxazole,and multiple-drug resistance was found among the isolates,the number of bacterial strains resistant to antibiotics were more between five and eight,the resisitance strains were 21.95% to 30.49%,7 isolates were even resistant to 13 antibiotics.The results showed the phenomenon of drug resistance of the isolates was very serious.