更新林相图的计算机辅助方法

常规编制与更新林相图的方法要求昂贵的光学机械纠正仪器、较高的操作技术和成本。文章提出一种利用计算机辅助纠正航空象片更新林相图的方法,可以降低成本,提高成图效率。为了实现这种方法,首先根据航空摄影理论分析,推导出适合编程的航空纠正数字化线性模型和算法。该模型要求在底图和航片上有四对控制点坐标,求算纠正参数。采用数字化仪测定这些坐标,求解参数,然后根据该线性模型对航片上编图要素的数字化坐标进行非共面变换,进而更新林相图。为完成这一过程需要四个程序模块和处理流程。使用这种方法更新林相图既能保证精度要求,又具有操作简单的优点。     

Landscape-ecological mapping of the Netherlands

The Landscape-ecological Mapping of the Netherlands project (LMN project) started in 1983 with the aim of establishing a landscape-ecological database for use in developing and evaluating national land-use plans. The project, working with grid cells of 1 km², has four working objectives: a) development of mapping potential for basic landscape-ecological data, b) assessment of susceptibility to interventions, c) evaluation of significance for nature conservation and d) production of vulnerability maps, as a combination of susceptibility and significance. In addition to information on soil, groundwater, ecotopes, flora and fauna, the database also incorporates information on physiographical features and entire landscapes. The resulting database is a geographic information system (GIS). This article describes the second phase of the project (1985–1989), covering the Randstad area, and focusses on the methods and the applications potential of the database.     

利用芥蓝×青花菜DH群体构建AFLP连锁图谱

 利用青花菜(Brassica oleracea var. italica) 与芥蓝(Brassica oleracea var. alboglabra) 杂交后代双单倍体(DH) 群体为材料, 通过AFLP技术构建了一个甘蓝类作物较高密度的遗传连锁图谱。该图谱包括9个主要连锁群及2个小连锁群, 总图距801.5 cM, 含337个AFLP标记, 标记间平均距离为3.6 cM,为甘蓝类作物基因定位、比较基因组学及重要经济性状QTL分析提供了一个框架图谱。     

利用大白菜DH群体构建AFLP遗传连锁图谱

 以大白菜‘汴早- 26’和‘光90E16’的F₁ 代进行游离小孢子培养所获得的含有59个株系的DH群体为试材, 利用AFLP技术通过63对引物筛选共获得346个AFLP多态性标记, 运用JoinMap 3.0软件构建大白菜遗传连锁图谱。该图谱主要包括10个连锁群, 总图距为708 cM, 平均图距为2.0 cM。     

草生栽培对(木奈)园温湿度和果实品质的影响

1999- 2 0 0 1年进行了草生栽培对园温湿度和果实品质影响的试验。结果表明 :草生栽培可以有效地改善果园生态环境 ,降低久旱暴雨后的裂果率 ,有利于提高果的商品质量和经济效益。     

Effect of mutant β-catenin gene on the proliferation of hepatocytes

AIM:The β-catenin is a key molecule in the Wnt signal pathway, which plays a critical role in normal development and tumorigenesis. However, the mechanisms of the β-catenin on the cell growth control are still not completely defined. The aim of this study was to test the hypothesis that the mutant β-catenin may regulate the hepatocyte proliferation. METHODS: The immortalized murine hepatocyte cell line, AML12, was used for this study. A plasmid that contain mutant β-catenin S33Y was transfected into the AML12 cells and a stable cell line AML12S33Y was established. The cell growth property of this cell line and the parental cell were compared by flow cytometry analysis and direct cell count. The cells were also tested for the ability to form soft agar colonies, and the ability to form tumors in the severe immune deficient mice (SCID). RESULTS:1. The mutant β-catenin containing cell line AML12S33Y has higher proliferating index compared with the parental AML12 cells (P<0.01), suggesting that mutant β-catenin promotes cell growth. 2. The mutant β-catenin cells formed small colonies in soft agar after 4 weeks of culture, but did not generate tumor in SCID mice. CONCLUSION:The mutant β-catenin promotes liver cell growth.     

Effect of tea-polyphenols on the activation of NF-κB and the expression of TGF-β1 mRNA in THP-1 cells incubated with VLDL,LDL or ox-LDL

AIM:To investigate the effect of tea-polyphenols (TP) on the activation of NF-κB and the expression of TGF-β₁ mRNA in THP-1 cells (a human acute monocytic leukemia cell line). METHODS:THP-1 cells were incubated with the different concentrations of TP, VLDL, LDL or ox-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-κB was detected with immunohistochemistry technique, the positive index of the TGF-β₁ mRNA expression was detected by hybridization in situ, and accumulation of total cholesterol (TC) in cells incubated with 0.4-40 μg/L TP was determined with oxidase assay. RESULTS:The nuclear malposition rate of NF-κB, the positive index of the TGF-β₁ mRNA expression and TC in THP-1 cells incubated with 0.4-40 μg/L of TP were lower than those with 0 μg/L of TP in TP-V group, TP-L group and TP-O (P＜0.05). The differences of these markers in THP-1 cells incubated with more than 40 μg/L TP in TP-V group, TP-L group and TP-O were not statistically significant, compared with TP-C group (P＞0.05). CONCLUSION:TP inhibited the activation of NF-κB, the expression of TGF-β₁ mRNA and the foam cell formation in the mono-macrophage.     

Predicting the spatial distribution of ground flora on large domains using a hierarchical Bayesian model

Accomodation of important sources of uncertainty in ecological models is essential to realistically predicting ecological processes. The purpose of this project is to develop a robust methodology for modeling natural processes on a landscape while accounting for the variability in a process by utilizing environmental and spatial random effects. A hierarchical Bayesian framework has allowed the simultaneous integration of these effects. This framework naturally assumes variables to be random and the posterior distribution of the model provides probabilistic information about the process. Two species in the genus Desmodium were used as examples to illustrate the utility of the model in Southeast Missouri, USA. In addition, two validation techniques were applied to evaluate the qualitative and quantitative characteristics of the predictions.This revised version was published online in May 2005 with corrections to the Cover Date.     

SSR技术及其在果树上的应用

SSR(Simple sequence repeat)技术以其丰富的多态性、共显性遗传、重复性好和操作简便等优点日益受到重视,已成为植物遗传和育种研究中不可缺少的分子标记。对SSR技术的原理和特点作了简要的介绍,较详细地分析了如何获得SSR引物,特别是综述了果树上SSR引物的研究现状,同时将其与其它几种主要的分子标记进行了比较分析,认为SSR标记检测的位点多态性水平明显高于RFLP,而且重复性优于RAPD;着重介绍了SSR技术在果树种质资源和构建果树遗传图谱及基因定位等研究中的应用现状;指出SSR技术将在果树科研上起到重要的作用。