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为建立倍他米松(BET)ELISA检测方法,先使BET与琥珀酸酐反应,再采用活化酯法与牛血清白蛋白(BSA)偶联制备免疫原;其次,利用免疫动物获得多克隆抗体,经Sephrose 4B-proteinA方法对抗体进行纯化;最后,建立倍他米松ELISA检测方法。结果表明,免疫抗原偶联成功,获得了亲和力较高的多克隆抗血清,间接竞争ELISA测定其滴度为102 400、IC15为6.60×10-5 ng/mL和IC50为0.97ng/mL。该方法适用于残留在动物性食品中的BET现场大批量检测。 相似文献
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Miguel Mellado Lucía M. Treviño María I. Chavez Francisco G. Véliz Ulises Macías-Cruz Leonel Avendaño-Reyes José E. García 《Reproduction in domestic animals》2023,58(1):39-47
The objective of this retrospective study was to assess the effect of receiving a single (n = 50,285) or double (n = 4392) artificial insemination (AI), 12 h apart, within a timed artificial insemination protocol on pregnancy per AI (P/AI) in nulliparous heifers (inseminated with either sex-sorted or conventional semen) and pluriparous Holstein cows in a commercial dairy herd. Also, this study aimed to investigate the relationship between temperature-humidity index (THI) and time of the first AI and fertility. Fertility of cows receiving two AI with normothermia (THI <68) was higher (p < .05) than cows receiving a single AI (42.9% vs. 36.4%). P/AI of cows receiving two AI with severe heat stress (THI >85) was higher (p < .05) than cows receiving a single AI (21.0% vs. 12.6%). Regardless of heat stress conditions, applying the first AI in the morning increased (p < .05) P/AI in cows with double AI than in cows whose first AI occurred in the afternoon (38.4 vs. 33.3%). With moderate heat stress, and sexed-sorted semen, P/AI to timed AI was higher (65.0 vs. 51.9%; p < .05) in heifers receiving double AI than those serviced once. It was concluded that double AI, 12 h apart, enhanced fertility at timed AI than herd mates with a single AI, particularly with heat stress at breeding. 相似文献
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裸子植物体细胞胚胎发生和人工种子的研究 总被引:8,自引:0,他引:8
该文介绍了一种作为森林种子的大规模增值的有效方法--裸子植物体细胞胚胎发生和人工种子的研制。其内容包括:(1)胚性愈伤组织的诱导与保存;(2)影响体胚发生的因素;(3)体胚的发育与成熟;(4)大规模培养体胚;(5)成熟体胚的干化、包理与人工种子生产;(6)体胚的萌发和转换。最后对人工种子的应用前景进行了讨论。 相似文献
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An algorithm of fault section diagnosis based on topology identification for distribution networks is presented. By decomposing the topologic matrix which describing the distribution network into two parts,one part only contain the complex coupling factor ,and the other ignore the complex coupling factor. Using this method, the fault zones in distribution network can be identified and isolated efficiently, and the vertexes of the zones can also be identified automatically. This approach adapts to the changefully network structures. 相似文献
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Chen HuaZhou Jiaqi 《保鲜与加工》1990,(4):1-6
Based on the maximum flow principle about networks, a network flow approach to reliability evaluation in large scale netwerks ty mixing inereasing flow with decreasing flow is proposed in this paper.This approach gets the initial flow of each branch in a r.etwork associated with a state of the retwork by decreasing flows of some branches correlative to another state of the network.As a result, the number of flow compute required can be reduced enormously.The numerical example shows that the approach is powerful. The comparison of this approach with the minimal cutset one is also given in this paper. 相似文献
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