平原河网区排涝计算研究

行洪排涝是平原河网区汛期的重要任务。利用MIKE11模型中的HD模块,以江苏省泗洪县城区水系为实例,建立了一维水动力学模型,并利用产汇流计算确定了模型的边界条件,对20年一遇暴雨条件下的城区河网水量进行了模拟计算。结果表明,在现有的排涝设施条件下,泗洪县城区河网能够满足设计暴雨下的排涝要求。     

在不同种植密度下双低油菜宁杂11号、宁油16号摘薹试验研究

采用三因素裂区试验设计,研究了种植密度和摘薹对双低油菜宁杂11号、宁油16号的菜薹产量、菜籽产量及净收益等的影响。结果表明:宁杂11号各处理的菜籽产量和净收益均显著高于宁油16号,但菜薹产量略低于宁油16号。随着种植密度提高,宁杂11号的净收益显著增加,但摘薹后净收益增加不大。宁油16号摘薹后净收益大幅度增加。摘薹后油菜生育期推迟、株高降低、总角果数减少、千粒重下降,但角果长度和每角粒数能显著增加。     

Self-incompatibility in Brassicaceae crops: lessons for interspecific incompatibility

Most wild plants and some crops of the Brassicaceae express self-incompatibility, which is a mechanism that allows stigmas to recognize and discriminate against “self” pollen, thus preventing self-fertilization and inbreeding. Self-incompatibility in this family is controlled by a single S locus containing two multiallelic genes that encode the stigma-expressed S-locus receptor kinase and its pollen coat-localized ligand, the S-locus cysteine-rich protein. Physical interaction between receptor and ligand encoded in the same S locus activates the receptor and triggers a signaling cascade that results in inhibition of “self” pollen. Sequence information for many S-locus haplotypes in Brassica species has spurred studies of dominance relationships between S haplotypes and of S-locus structure, as well as the development of methods for S genotyping. Furthermore, molecular genetic studies have begun to identify genes that encode putative components of the self-incompatibility signaling pathway. In parallel, standard genetic analysis and QTL analysis of the poorly understood interspecific incompatibility phenomenon have been initiated to identify genes responsible for the inhibition of pollen from other species by the stigma. Herewith, we review recent studies of self-incompatibility and interspecific incompatibility, and we propose a model in which a universal pollen-inhibition pathway is shared by these two incompatibility systems.     

Chinese urban migrants' sense of place: Emotional attachment,identity formation,and place dependence in the city and community of Guangzhou

In this paper, we study urban migrants' sense of place in Guangzhou, China, focusing on the structural relations between place attachment, identification and dependence as the three key place dimensions. Through both quantitative structural equation modelling and qualitative analysis of in‐depth interviews data, our research suggests that migrants' sense of place demonstrates complex relationships between the three registers of emotional attachment, identity formation and functional dependence. The construction of sense of place is also related to the personal experiences of living as urban ‘outsiders’. Our research also reveals a striking difference between the city and community levels in terms of the ways in which migrants' sense of place is constructed. Urban migrants tend to exploit the functional utilities of microscopic urban spaces to meet their demands for recreation, education and socialisation. On the other hand, their sense of place to the city is largely compromised by their attachment to the hometown and conditioned by their personal identification to the city.     

超级稻新品种淮稻11号的选育及高产栽培技术

[目的]为徐淮地区优质水稻新品种的选育和生产利用提供技术支持。[方法]供试种子参加了2004～2007年江苏省中熟中粳稻预备试验和区域试验及迟熟中粳稻生产试验。[结果]徐淮11号在预备试验中的平均产量为9375.0kg/hm2;在区域试验中的平均产量为8682.0kg/hm2;在迟熟中粳稻生产试验中的平均产量为9439.5kg/hm2。淮稻11号在大面积示范试种中的一般产量为9750.0kg/hm2。淮稻11号分蘖成穗率和颖花分化能力均较高,有效穗数为285万穗/hm2,每穗实粒数为120～130粒,千粒重高达28.0～29.0g,结实率为90.0%。淮稻11号的糙米率为84.3%,整精米率为72.0%,垩白率为10.0%,垩白度为1.0%,碱消值为7.0,直链淀粉含量为18.4%,胶稠度为76mm,透明度为2级,综合品质评价达国标2级优质米标准。[结论]该试验成功选育出了超级稻新品种淮稻11号并为其推广种植提供了技术指导。     

工业化大豆蛋白7S和11S组分分离技术研究进展

该文围绕目前工业化生产11S和7S组分的分离技术成果,结合实验室的分离技术(Guo法),比较了各种分离方法的差异.结果表明:分离提取技术利用的原理几乎均建立在"碱溶酸提"和"冷沉"作用基础之上.Wu首次成功地进行了7S和11S的工业化分离,之后的研究多是对此方法的改进和修饰.而Guo法是唯一采用中性条件抽提的工业化分离方法,在节省了原料碱的同时,避免了蛋白的变性.同时,该文提出了目前工业化生产大豆组分蛋白中存在的问题,虽然不断在加工方式上进行改进,但仍存在步骤复杂、产率低、生产成本高等方面的问题,因此作者进一步对实现工业化分离大豆组分蛋白提出了需要改进和努力的方向.     

血清Ⅰ型马立克氏病病毒被膜蛋白UL11在真核细胞内的表达

本研究利用血清Ⅰ型马立克氏病病毒（Marek＇s disease virus serotype 1,MDV-1）被膜蛋白UL11原核表达产物作为免疫原,免疫小鼠制备多抗血清,通过间接免疫荧光试验,结果发现：UL11真核表达时,存在于COS-1和Sf9细胞浆中。而检测MDV的RB1B毒株感染CEF时,却发现UL11高度聚集于空斑中心,且可分布于空斑周围的所有细胞核中。这为研究UL11细胞核内扩散的分子机制,以明确UL11是否参与MDV-1致瘤的过程提供了参考。     

杂交玉米玉11特征性及高产栽培技术

杂交玉米(Zea maysL.)华玉11是华中农业大学刘永忠教授选育的玉米新品种,具有高抗、高产、稳产特性,是适合山区种植、综合性状优良的强优势杂交春玉米,该品种自2013年推广以来,在湖北、云南四川及湖南等地种植表现出高抗、高产、稳产特性.     

葛渣膳食纤维的过氧化氢改性工艺研究

为研究过氧化氢对葛渣膳食纤维的改性作用,以葛根加工淀粉后的残渣为原料,采用单因素与正交试验,以可溶性膳食纤维得率以及不溶性膳食纤维膨胀力为指标,得出了过氧化氢改性制备葛渣膳食纤维的最佳工艺条件,即H_2O_2体积分数0.3%,pH为12,温度为90℃,时间为75 min,得到可溶性膳食纤维得率最高为52.16%,不溶性膳食纤维膨胀力为7.01 m L/g。     

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为构建抗虫和耐除草剂转基因玉米Bt11的多重PCR检测方法,根据抗虫和耐除草剂玉米Bt11分子特征,同时选择玉米内源参照基因zSSIIb、外源基因Cry1Ab、P-CaMV 35S、T-NOS和PAT 5个基因作为MPCR检测基因,参照国家相关标准中特异性引物序列,通过对反应条件的优化以及方法特异性和灵敏度测试,建立MPCR检测体系.结果表明:利用已知样品对体系(退火温度为56℃,模板浓度≥200bp,引物配比为zSSIIb∶Cry1Ab∶P-CaMV 35S∶T-NOS∶PAT =0.1∶0.2∶0.2∶0.2∶0.2)验证,抗虫和耐除草剂玉米Bt11能同时被检出zSSIIb、Cry1Ab、P-CaMV 35S、T-NOS和PAT等5个基因,而其他样品均不能被同时检出这5个基因,建立体系可用于抗虫和耐除草剂转基因玉米Bt11检测.