湘南典型残次阔叶林人工促进更新技术研究

于2003年在湖南省汝城县益将国有林场天然残次阔叶林内设置试验样地和对照样地各3个,采用林地清理、补植、补播等手段促进天然更新,并分别于2006年和2011年进行2次人工促进干预;同时,在6个样地内各设置3个样方,共计18个样方,分别于2003、2008、2013年冬,对幼树、幼苗、萌条的数量及地径进行统计,并对样方内胸径≥5 cm的立木进行每木调查。结果表明:种子补播保存率43%,幼苗补植保存率20%,平均保存率32%。树高≤30 cm且地径0.5 cm、31 cm≤树高≤50 cm且0.5 cm地径0.7 cm幼树的数量下降率分别是对照样地的17.5%、21.59%,树高≥51 cm且地径0.7 cm幼树的数量下降率是对照样地的121.66%。2008年单位面积蓄积量是对照样地的2.28倍,2013年达到3.80倍;经过10年更新,单位面积蓄积量是试验前的11.57倍,对照样地单位面积蓄积量仅是试验前的2.55倍;单位面积蓄积量是对照样地的4.54倍。人工促进天然更新所需的人工成本为1 860元/hm2,仅为同期人工营造杉木幼林所需人工成本5 310元/hm2的35%。     

云南省古林箐自然保护区物种多样性及保护

古林箐自然保护区位于北回归线以南,哀牢山南端以东的滇东南高原向东南半岛延伸的结合部,南亚热带与热带的过渡地带。在这特殊的自然地理条件下,成为我国三大生物多样性中心之一的滇东南—桂西北的核心部位,区内有发育完整,保存完好的森林生态系统和丰富的生物资源。但在管理中存在许多方面问题,据此提出保护措施。     

HPLC法测定天然植物粗提物中芍药苷含量

建立高效液相色谱法检测天然植物粗提物饲料原料中芍药苷的测定方法。用十八烷基硅烷键合硅胶色谱柱,以乙腈-0.1%磷酸溶液(14:86)为流动相,检测波长230nm,流速：1.0 mL/min;柱温：30 ℃;进样量：10μL。结果显示,芍药苷的线性方程为Y=10.6017X－14.8514,(r=0.99966,n=6),本方法的回收率为100.4~102.1%,RSD均小于2.0%。结果表明,本方法准确,可靠,适用于天然植物粗提物饲料原料中芍药苷的测定。     

非洲猪瘟自然弱毒株演变进程

自2018年非洲猪瘟(African swine fever,ASF)传入中国以来疫情得到了有效控制。但2021年以后,我国的ASF流行情况出现了新的变化,临床中出现了“自然变异株”。这些毒株可能导致感染后临床症状不典型,容易与其他疫病混淆等问题。这与国外的流行演变规律一致,即当ASF在一个国家或地区流行较长时间后,其临床表现将由急性发病转变为缓慢发病或出现新的临床表现。经过多年的观察积累,国外已经对该病毒的流行规律特别是自然弱毒株的演变进程有了大量记载,但国内目前尚无这方面的描述。为此,就ASF自然弱毒株的演变进程进行综述,从而提出加强血清学诊断产品研发、加快产业化报批进程、适时开展血清学监测追溯等建议,以期为我国ASF科学防控提供参考。     

基于CFD的Venlo温室夏季组合降温措施模拟研究

针对我国南方地区夏季高温高湿的气候条件,对采用天窗、外遮阳、内喷雾降温措施的试验Venlo温室内温度状况进行了模拟研究。以室外气候参数为边界条件,考虑作物和环境的相互作用,内喷雾系统和室内环境的质热交换以源项的形式加入到控制方程中,采用CFD(computational fluid dynamics)中的稳态方法求解控制方程,模拟Venlo型温室不同调控措施及组合下的温室内温度分布特点,分析各种调控措施的调控效果。模拟结果表明:采用加入源项的方法模拟内喷雾系统和室内空气的质热交换,其模拟值和实测值均方根误差RMSB为0.514 4℃,最大绝对误差为0.75℃,平均相对误差为1.3%,说明所建立的CFD模型有效。3种降温措施下,以外遮阳+自然通风的降温贡献率最大(80.6%),能耗最高的喷雾系统降温贡献率仅为34.8%,较高的环境湿度影响了喷雾系统的降温效率。CFD夏季降温模型的建立为温室作物系统的环境控制策略的制定提供了科学依据。     

基于ANSYS Workbench的乘坐式割草机刀盘模态分析

刀盘是割草机重要部件,其工作时固有频率与振型的大小直接关系到整机的可靠性与安全性。为此,利用CAD/CAM软件Pro/E建立刀盘的三维实体模型,并将其导入有限元分析软件ANSYS Workbench,对所建模型进行模态有限元仿真分析,提取前6阶固有频率,与刀盘转速进行比较,计算出其工作时的危险转速范围。分析结果表明:刀盘最高工作转速远低于其1阶临界转速,有效地避免了共振的发生,验证了刀盘设计的合理性,可为刀盘其它的动力学分析奠定基础。     

昆虫病原真菌在害虫防治中对天敌生物的影响研究进展

昆虫病原真菌是一类重要的微生物杀虫剂,广泛应用于农林害虫的生物防治。由于寄主范围广和主动侵染的特点,昆虫病原真菌在侵染、致死靶标害虫的同时,对包括天敌昆虫在内的非靶标节肢动物等会产生潜在的致病或亚致死风险。本文总结了昆虫病原真菌在害虫防治中的研究进展及对天敌生物的影响,并展望了昆虫病原真菌作为极具潜力的生物农药与天敌生物联合防治害虫的未来发展方向。     

Genetic and non-genetic factors influencing KLH binding natural antibodies and specific antibody response to Newcastle disease in Kenyan chicken populations

This study aimed at investigating the influence of genetic and non-genetic factors on immune traits to inform on possibilities of genetic improvement of disease resistance traits in local chicken of Kenya. Immune traits such as natural and specific antibodies are considered suitable indicators of an individual's health status and consequently, used as indicator traits of disease resistance. In this study, natural antibodies binding to Keyhole Limpet Hemocyanin (KLH-NAbs) was used to measure general disease resistance. Specific antibodies binding to Newcastle disease virus (NDV-IgG) post vaccination was used to measure specific disease resistance. Titers of KLH-NAbs isotypes (KLH-IgM, KLH-IgG and KLH-IgA) and NDV-IgG were measured in 1,540 chickens of different ages ranging from 12 to 56 weeks. A general linear model was fitted to determine the effect of sex, generation, population type, phylogenetic cluster, line, genotype and age on the antibody traits. A multivariate animal mixed model was fitted to estimate heritability and genetic correlations among the antibody traits. The model constituted of non-genetic factors found to have a significant influence on the antibody traits as fixed effects, and animal and residual effects as random variables. Overall mean (±SE) concentration levels for KLH-IgM, KLH-IgG, KLH-IgA and NDV-IgG were 10.33 ± 0.04, 9.08 ± 0.02, 6.00 ± 0.02 and 10.12 ± 0.03, respectively. Sex, generation and age (linear covariate) significantly (p < 0.05) influenced variation across all the antibody traits. Genotype effects (p < 0.05) were present in all antibody traits, apart from KLH-IgA. Interaction between generation and line was significant (p < 0.05) in KLH-IgM and NDV-IgG while nesting phylogenetic cluster within population significantly (p < 0.05) influenced all antibody traits, apart from KLH-IgA. Heritability estimates for KLH-IgM, KLH-IgG, KLH-IgA and NDV-IgG were 0.28 ± 0.08, 0.14 ± 0.06, 0.07 ± 0.04 and 0.31 ± 0.06, respectively. There were positive genetic correlations (0.40–0.61) among the KLH-NAbs while negative genetic correlations (−0.26 to −0.98) were observed between the KLH-NAbs and NDV-IgG. Results from this study indicate that non-genetic effects due to biological and environmental factors influence natural and specific antibodies and should be accounted for to reduce bias and improve accuracy when evaluating the traits. Subsequently, the moderate heritability estimates in KLH-IgM and NDV-IgG suggest selection possibilities for genetic improvement of general and specific immunity, respectively, and consequently disease resistance. However, the negative correlations between KLH-NAbs and NDV-IgG indicate the need to consider a suitable approach that can optimally combine both traits in a multiple trait selection strategies.     

A STUDY ON HEAT AND MOISTURE STATE OF HULL LAYER FOR THERMAL INSTULATION OF A COLD STORAGE

This paper analyses the thermal moist characteristics of the hull layer beside an air layer at lower temperature in an envelope for a cold storage and presents a new method of waterproof by air layer. With test data from engineering practice, the theory on the barrier of self moisture proof at the surface sublayer of the hull layer in an attic on a cold storage is proven. The dried zone about three phase point is confirmed. It is a new knowledge for thermophysics on thermal design of a cold storage with an optimum thermal performance.     

景观生态学原理在河道生态岸堤构

分析了传统水工设计中岸堤(岸坡)构建的弊端、简要介绍了国内外学者在河流的生态整治方面的思想与观点。从景观生态学的角度着重介绍了岸堤(岸坡)生态系统的组成和主要特征、生态岸堤的多种功能和景观生态学内涵；提出了在生态岸堤设计中的坚持生态优先，同时重视景观性和稳定性；根据使用材料的不同对岸堤进行分类,对生态岸堤的植物群落的构建进行分析。本文可为从而为生态岸堤的构建、河流、洪泛区的治理提供一定的思路和参考。