甘露糖修饰的壳聚糖聚乳酸-羟基乙酸共聚物纳米微球作为口蹄疫病毒核酸疫苗递送载体的特性评价

旨在对制备的甘露糖修饰的壳聚糖聚乳酸-羟基乙酸共聚物[poly （D，L-lactide-co-glycolide），PLGA]纳米微球作为口蹄疫病毒（foot-and-mouth disease virus，FMDV）核酸疫苗递送载体进行评价。采用西佛碱反应和元素分析制备具有一定取代度的甘露糖修饰的壳聚糖衍生物（mannose modified chitosan，MCS），然后，经双重乳化挥发法制备得到甘露糖修饰的壳聚糖PLGA纳米微球（MCS-PLGA-NPs）。采用纳米粒径仪检测MCS-PLGA-NPs粒径分布和表面电势（zeta）、扫描电镜考察其形态、琼脂糖凝胶电泳观察其对质粒的吸附和吸附质粒后抵抗核酸酶降解能力、CCK-8法检测MCS-PLGA-NPs的细胞毒性、激光共聚焦观察巨噬细胞对MCS-PLGA-NPs-质粒DNA复合物的摄取、荧光显微镜和Western blot验证MCS-PLGA-NPs加载质粒DNA在细胞中的表达。元素分析结果表明，成功制备了取代度为5%~10%的MCS。纳米粒径测定和扫描电镜结果表明，MCS-PLGA-NPs的zeta为正值、粒径分布均匀且形态规则呈球形。琼脂糖凝胶电泳结果显示，MCS-PLGA-NPs吸附质粒的能力随着其质量的增加而增强并且可以在一定程度上抵抗核酸酶降解质粒DNA。在细胞毒性试验中，不同浓度的MCS-PLGA-NPs与RAW264.7细胞共孵育24 h后，细胞存活率仍在85%以上。在细胞摄取试验中，用激光共聚焦显微镜可以明显观察到质粒DNA结合到纳米微球表面被RAW264.7细胞摄取。荧光显微镜和Western blot试验证明MCS-PLGA-NPs加载质粒DNA可以在细胞中进行表达。综上表明，本研究成功制备了MCS以及具有递送核酸疫苗能力的MCS-PLGA-NPs，为FMDV核酸疫苗的递送研究提供了新的方向和见解，也为该递送载体携带特定抗原靶向抗原递呈细胞表面甘露糖受体以及应用于动物免疫的研究奠定基础。     

Preparation of Alginate-Based Biomaterials and Their Applications in Biomedicine

Alginates are naturally occurring polysaccharides extracted from brown marine algae and bacteria. Being biocompatible, biodegradable, non-toxic and easy to gel, alginates can be processed into various forms, such as hydrogels, microspheres, fibers and sponges, and have been widely applied in biomedical field. The present review provides an overview of the properties and processing methods of alginates, as well as their applications in wound healing, tissue repair and drug delivery in recent years.     

Marketing mix for the promotion of biological control among small-scale paddy farmers

Understanding farmers’ decision process on the adoption of biological control (BC) may help in promoting adoption. This study explored for the first time the impact of marketing mix elements (product, price, promotion, and place) on bolstering BC for the management of rice stem borer (Chilo suppressalis Walker) among rice farmers in Fuman County in northern Iran. Farmers using BC were familiar with this method primarily via village councils and rural municipality organizations (Dehyaris) and then via distributors of trichocards. Almost half of the farmers (48.3%) had weak to moderate level of contact with distributors of trichocards. All four elements (4Ps) of marketing mix (product, place, price, and promotion) were effective on the marketing of BC agents. Place (distribution) mix, followed by price mix, were the most important factors in marketing trichocards in farmers’ views. According to the sensitivity to 4Ps of marketing mix, most farmers were grouped as semi-sensitive (45.6%) and sensitive (39.4%). Overall, the availability of biological agent products in the market, the affordable price of the products, and the strong relationship of rural people with village councils and rural municipality organizations (Dehyaris) are crucial factors for BC promotion in paddy fields of Fuman.     

水锤泵进水系统的试验与分析

着重对水锤泵的进水管直径,倾角及管长进行试验分析,从而提高了水锤泵的提水效率,增强了其适用性。     

Integrated assessment of catchment suspended sediment budgets: a Zambian example

In many developing countries, the management of sediment‐related environmental problems is severely hampered by a lack of information on sediment mobilization and delivery in river basins. The sediment budget concept represents a valuable framework for assembling such information, which can, in turn, be used to assist with the design and implementation of soil erosion and sediment control policies. However, the information necessary to construct a catchment sediment budget is difficult to assemble. Against this background, an integrated approach to establishing a catchment suspended sediment budget, involving a river monitoring station, the use of ¹³⁷Cs measurements to estimate soil erosion and deposition and floodplain accumulation rates within the catchment, and sediment source fingerprinting, has been developed and tested in the 63 km² catchment of the upper Kaleya River in southern Zambia. The approach developed not only provides detailed information on individual components of the suspended sediment delivery system, but also permits the establishment of the overall catchment sediment budget. A sediment budget for the upper Kaleya catchment is presented and both its key features and its wider implications for catchment management are discussed. Copyright © 2001 John Wiley & Sons, Ltd.     

Simulation of automatic control of an irrigation canal

Improved water management and efficient investment in the modernization of irrigation schemes are essential measures in many countries to satisfy the increasing demand for water. Automatic control of the main canals is one method for increasing the efficiency and flexibility of irrigation systems. In 2005, one canal in the irrigation scheme ‘Sector B-XII del Bajo Guadalquivir’ was monitored. This canal is representative of irrigation schemes in Southern Spain; it is divided into four pools and supplies an area of 5154 ha. Ultrasonic sensors and pressure transducers were used to record the gate opening and water levels at the upstream and downstream ends of each canal pool. Using the recorded data and the SIC (Simulation of Irrigation Canals) hydraulic model, two canal control options (local upstream control and distant downstream control) were evaluated using a PI (Proportional-Integral) control algorithm. First, the SIC model was calibrated and validated under steady-state conditions. Then the proportional and integral gains of the PI algorithm were calibrated. The controllers were tested using theoretical demand changes (constant outflow followed by a sudden demand increase or decrease) and real demand changes generated on the basis of a spatially distributed crop water balance that included a number of sources of variability (random and not random) in the determination of field irrigation timing and depth. The results obtained show that only the distant downstream controller was able to adjust quickly and automatically the canal dynamics to the varying water demands; it achieved this efficiently and with few spills at the canal tail, even when there were sudden and significant flow variations.     

Deforestation effects on soil degradation and rehabilitation in western Nigeria. IV. Hydrology and water quality

Hydrological and water-quality measurements were made on a 44·3 ha watershed under forest cover and following deforestation and conversion to an agricultural land-use. Under secondary tropical rainforest, water yield ranged from 2·2 per cent to 3·1 per cent of annual rainfall. Deforestation of 7 per cent of the watershed area increased water yield to 7·0 per cent of annual rainfall. Baseflow increased with deforestation, and increased progressively with time after deforestation. It was 5·1 per cent of annual rainfall in 1979, 15·1 per cent in 1980, 16·4 per cent in 1981 and 17·9 per cent in 1982. In comparison, surface flow was 4·5 per cent in 1979 and 6·2 per cent in 1980, but decreased to 2·3 per cent in 1981 and 2·4 per cent in 1982. Total water yield following deforestation and conversion to agricultural land-use ranged from 9·6 per cent to 21·3 per cent of the annual rainfall received. The dry season flow decreased with time as the dry season progressed, but increased over the years following deforestation. Surface runoff during the rainy season depended on ground cover and soil quality. The extent and severity of soil degradation affected the dynamics of surface flow. Because of actively growing crops, plant nutrient concentrations in surface runoff were low. Forested lysimeters had higher seepage losses than cropped lysimeters, and the water-use efficiency was 1·9–3·6 kg ha⁻¹ mm⁻¹ for cowpeas compared with 6·1–11·0 kg ha⁻¹ mm⁻¹ for maize. The delivery ratio was high immediately after deforestation and decreased to a steady value of about 3·2 per cent within 7 years. The data show five distinct phases of soil degradation in relation to generation of surface runoff. © 1997 John Wiley & Sons, Ltd.     

Protection of Fenneropenaeus chinensis (Osbeck, 1765) against the white spot syndrome virus using specific chicken egg yolk immunoglobulins by oral delivery

Two kinds of specific chicken egg yolk immunoglobulins (IgYs), IgY‐WSSV and IgY‐VP28, were, respectively, raised against the 2 mM binary ethylenimine (BEI)‐inactivated white spot syndrome virus (WSSV) and a principal envelope protein VP28. The activity of purified specific IgYs was stable under the conditions of 20–70 °C, pH 3.0–10.0 and 0–700 g L^?1 sucrose solution. In the neutralization assay, these high‐affinity IgY antibodies can specifically bind with the virus particles to protect shrimp (Fenneropenaeus chinensis) against WSSV infection. After oral delivery for 20 days, the IgY‐WSSV exerted a higher protection effect (RPS: 71.5%) than IgY‐VP28 (RPS: 63.7%). Moreover, an increase in RPS (79.2%) was found on addition of IgY‐WSSV:VP28 (0.1% IgY‐VP28 plus 0.2% IgY‐WSSV). This may indicate that neutralization of WSSV refers to the multiple‐hit model. By time‐course study of the levels of the specific IgYs in vivo, the data showed that the titre was enhanced to a relatively high level (P/N=8.35±0.45) at 3 days post administration, declined slightly (P/N=7.13±1.01) at 7 days post administration and then remained stable for further investigation. The stable antibody level potentially contributes towards blocking a large number of WSSV particles from entering and infecting on the major tissues at the early and late stages after challenge in shrimp.     

Synthesis and Characterization of 2-Decenoic Acid Modified Chitosan for Infection Prevention and Tissue Engineering

Chitosan nanofiber membranes are recognized as functional antimicrobial materials, as they can effectively provide a barrier that guides tissue growth and supports healing. Methods to stabilize nanofibers in aqueous solutions include acylation with fatty acids. Modification with fatty acids that also have antimicrobial and biofilm-resistant properties may be particularly beneficial in tissue regeneration applications. This study investigated the ability to customize the fatty acid attachment by acyl chlorides to include antimicrobial 2-decenoic acid. Synthesis of 2-decenoyl chloride was followed by acylation of electrospun chitosan membranes in pyridine. Physicochemical properties were characterized through scanning electron microscopy, FTIR, contact angle, and thermogravimetric analysis. The ability of membranes to resist biofilm formation by S. aureus and P. aeruginosa was evaluated by direct inoculation. Cytocompatibility was evaluated by adding membranes to cultures of NIH3T3 fibroblast cells. Acylation with chlorides stabilized nanofibers in aqueous media without significant swelling of fibers and increased hydrophobicity of the membranes. Acyl-modified membranes reduced both S. aureus and P. aeruginosa bacterial biofilm formation on membrane while also supporting fibroblast growth. Acylated chitosan membranes may be useful as wound dressings, guided regeneration scaffolds, local drug delivery, or filtration.     

图书馆文献传递服务浅谈

分析了图书馆开展文献传递服务的必然性,介绍了福州大学图书馆当前文献传递服务发展的现状。