机器视觉技术在精细农业中的研究进展

精细农业以节约投入、增加产出、提高投入物利用率、减少环境污染为目的.快速、准确地采集各种农田信息,有效地监测农业对象是实施精细农业的重要基础.机器视觉技术由于其非破坏性、精度高、成本效率高、信息量大、灵活等特点,在精细农业中得到了广泛的应用.为此,通过对大量参考文献进行分析,发现机器视觉在精细农业中的主要研究方向集中在农业机械自动导航、作物生长信息检测、变量控制等方面.同时,对机器视觉技术在上述领域中的研究情况进行分析和总结,并讨论了未来机器视觉技术在精细农业中应用存在的问题以及发展前景.     

三种不同变量施肥执行机构的比较研究

目前,国内外有3种变量施肥执行机构。为此,通过对3种国内外变量施肥执行机构的设计方案进行比较研究,分析出各自的特点,选择出适合黑龙江垦区国产大豆精密播种机的变量施肥执行机构,为设计研制适合国产大豆精密播种机的变量施肥执行机构创造条件。     

金属带式无级变速器的速比控制

在分析了速比控制阀特性的基础上，考虑到无级变速传动系统的复杂性，设计了模糊控制器，开发了基于速比反馈的速比控制系统，并进行了台架模拟试验。试验结果表明，实际速比能够较好地跟踪目标速比的变化，所设计的控制系统能够满足实际控制的需要。     

立轴柱形变螺距螺旋开沟机临界转速分析

通过对螺旋开沟机所输土块的运动及受力情况进行分析可知：当土块与坑壁之间的摩擦力能阻止土块随叶片旋转时，土块在螺旋叶片上所受作用力相互平衡。由此，推导山立轴柱形变螺距螺旋开沟机的临界转速。此即为保证土壤在螺旋而上上升的基本条件，而实际转速则大于临界转速。该分析为进一步设计立轴柱形变螺距螺旋开沟机提供了依据。     

基于ITS序列的曼陀罗属植物的分类学研究

根据曼陀罗属核糖体基因转录间隔区(ITS)序列设计通用引物,得到33个不同来源的曼陀罗属植物的ITS序列,并以小天仙子(Hyoscyamus bohemicus)为外群,应用遗传距离与系统树分析法对曼陀罗属植物之间的分类进行了初步探讨。结果表明:在供试样品中,紫花曼陀罗(Datura tatula)和曼陀罗(Daturastramonium)的ITS序列完全相同;多刺曼陀罗(Datura ferox)、栎叶曼陀罗(Datura quercifolia)和曼陀罗(Datura stramonium)之间的亲缘关系很近;紫花曼陀曼(Datura tatula)、曼陀罗(Datura stramonium)、无刺曼陀罗(Datura inermis)和光滑曼陀罗(Datura laevis)4个种间不存在遗传距离;传统分类中曼陀罗属的Dutra亚属内除光曼陀罗(Datura leichhardtii)和异色曼陀罗(Datura discolor)在ITS序列表现为比较独立的2个种外,其他各种间的亲缘关系也较近;在ITS序列上湿地曼陀罗(Datura ceratocaula)与曼陀罗(Daturastramonium...     

加强农作物种子质量监督抽查 保证用种安全和规范经营行为

近年来,由于种子市场竞争激烈,种子质量纠纷案件有所上升,以次充好、假冒伪劣种子案件时有发生,扰乱了种子市场秩序,危害了农业生产安全,增加了社会的不稳定因素。因此,加强农作物种子的监督抽查,防止不合格种子流入市场,确保农业生产安全和社会稳定具有重要意义。     

Random amplified polymorphic DNA markers reveal low genetic variation and a single dominant genotype in Eichhornia crassipes populations throughout China

Genetic variation within and between 34 populations of Eichhornia crassipes (water hyacinth) in China was surveyed using random amplified polymorphic DNA (RAPD) markers. A total of 1009 individuals were analysed, for which 12 RAPD primers amplified 69 reproducible bands, with 22 (32%) being polymorphic. The percentage of polymorphic loci (p) within a population ranged from 4.4% to 17.4%, and the mean Nei's gene diversity (H_e) was 0.046 ± 0.0145, indicating a low genetic diversity of E. crassipes in China. Each population contained at least four RAPD phenotypes (genotypes), and the same particular genotype was invariably dominant in all the populations sampled. The mean proportion of distinguishable genotypes was 0.29. Analysis of molecular variance revealed a large proportion of genetic variation (83.9%) residing within populations and a slightly larger proportion (88.1%) within localities, indicating a low genetic differentiation of E. crassipes populations, both locally and regionally. Human-mediated dispersal, vigorous clonal growth, and a generally low level of sexual reproduction were thought to be responsible for such a pattern of genetic structure.     

Phylogenetic Analysis of Field Isolates of Feline Calcivirus (FCV) in Japan by Sequencing Part of Its Capsid Gene

The molecular epidemiology of the infectious disease caused by feline calcivirus (FCV) in Japan was investigated by analysing the phylogenetic relationship among 21 Japanese field isolates, including the F4 strain, and 30 global isolates. Parts of the capsid gene (B–F) of the isolates were amplified by RT-PCR, and the amino acid sequences were compared with those from the global isolates. Thirty-seven and 14 out of a total of 51 isolates were clustered into two distinct genogroups, I and II respectively, by UPGMA and NJ analysis. Seven of the 21 Japanese isolates (33%) fell into group I together with 30 global isolates, while the other 14 Japanese isolates (67%) belonged to group II. The bootstrap repetition analysis of groups I and II formed by the NJ method gave a value of 99.0%. The 14 latter Japanese isolates were clearly separated from the isolates in group I, and they were different from any previously known FCV, forming a new genogroup, which implies that this lineage has been confined to Japan. Comparing the amino acid sequences shared by groups I and II, the amino acid at position 377 in B region was asparagine (Asn or Asp (NH₂)) in group I, while it was lysine (Lys) in all the strains in group II. Similarly, the amino acid at position 539 in the F region was alanine (Ala) or proline (Pro) in group I, while it was valine (Val) in group II; glycine (Gly) at position 557 in group I was serine (Ser) in Group II; and phenylalanine (Phe) or leucine (Leu) at position 566 in genogroup I was tyrosine (Tyr) in group II.     

Comparison of homogeneity and heterogeneity of residual variance using random regression test-day models for first lactation Japanese Holstein cows

Using a large‐scale data set that included first lactation test day records from 1975 to 2000 for Japanese Holsteins, genetic parameters for milk yield were estimated by using random regression (RR) test‐day models (TDM) with heterogeneous and homogeneous residual variances. It is necessary for the RR‐TDM to include a function that explains the shape of the lactation curve. The RR‐TDM with the LW curve, which combined Wilmink's curve and a Legendre polynomial, was used for fitting the model for milk yield. In recent years, increases in residual variance have been noted for Japanese dairy cattle. Thus, three kinds of heterogeneous residual variance over the calving year were considered: H1, H2 and HG. Linear and quadratic exponential functions for the calving year were used in H1 and H2, respectively. Residual variance of HG was divided into five groups according to calving year. Homogeneous residual variance was HO. All heterogeneous residual variances increased with calving year in an almost linear fashion. Residual variance increased over the study period. However, there is no need to consider heterogeneous residual variances in genetic evaluations, because the heterogeneity of residual variance over the years did not affect the ranking of top sires and cows.