Tissue Culture and Rapid Propagation of Actinidia chinensis

It has been briefly studied and reported that Actinidia chinensis , one of economic woody plants, has been bred with the method of plant tissue culture. Aim at the shortage of traditional plant tissue culture ways which ignored the refinement and transplant of test-tube seedling, it strengthen the study to breeding and management of Actinidia chinensis seedlings before they grow up and become mature seedlings. Through our explore, the transplant survive rate of tissue-culture seedlings attain to about 85%.This study is the improvement to traditional breeding seedling means and it is helpful to the rapid propagation of those economic woody plants.     

Tissue Culture and Rapid Propagation of Echinacea Purpurea

It is a burgeoning application field for the biotechnology that to apply the technique of plant tissue culture breed seedling. The authors study the responses of Echinacea purpurea to different proportion plant growth hormone or auxin through adopting the culture tissue technique on the MS. The integrated seedling of Echinacea purpurea can be cultivated by callus, subculture, differentiation and rhizogenesis. The surviving rate of Echinacea purpurea seedling transplant are improved by appropriate sound stimulation appropriate. Study harvest offers the gist for the large-scale breeding seedling and the officinal resource exploitation of Echinacea purpurea in our country, and avoid some traditional handicap in breeding seedling of Echinacea purpurea, such as the difficult of seed collection, low burgeoning rate, and the disadvantage for large-scale propagation.     

Physiological and pathological roles of mast cells in adipose tissue

Mast cells are important cells for the innate immunity that reside in tissues including adipose tissue and are involved in various physiological and pathological processes by producing a range of biological mediators. Adipose tissue not only acts as an energy depot and regulator of energy homeostasis that can deposit excess energy and dissipate energy through heat, but also is an active endocrine organ capable of producing hormones and adipokines. The dysfunction of adipose tissue is highly correlated with metabolic disorders, such as obesity, type 2 diabetes mellitus and so on. This review focuses on the physiological and pathological roles of mast cells in adipose tissue.     

水曲柳的组织培养

以水曲柳休眠冬芽及萌动后新芽和带芽茎段为材料,进行组织培养。冬芽消毒以用0.1%升汞去芽鳞前后各5min3、min为好,以SH、WPM附加不同激素可诱导冬芽的萌动。对于新芽,以SH为基本培养基,附以BA、NAA及谷氨酸,可诱导愈伤组织及芽枝的生成。WPM效果不如SH。     

魔芋组织培养与植株再生的研究

魔芋幼芽和幼嫩芽鞘,经0.1%升汞灭菌20分钟后,接种在附加BA.NA(均为0.6m/1)的MS培养基上,在室温25土1℃,每天照光9小时的条件下培养三周,形成愈伤组织。愈伤组织转入附加4(mg/1)BA、0.25(mg/1)IBA,3(mg/1)GA_3的MS分化培养基上,四周后分化出芽和白色地下茎,其分化颖率为40%,再转移至附加2(mg/1)BA、1(mg/1)IBA、0.3%活性炭的MS培养基中,幼茎和叶片从芽鞘中抽出,茎基部分化出根,形成完整的小植株。     

不同激素对碧云茶树丛生芽增殖培养的影响实验

为了获得碧云茶树的组培技术,加快其优良品种繁殖速度,2010～2011年进行了不同浓度的细胞分裂素6-BA和生长素NAA对碧云茶树丛生芽增殖培养的影响。结果表明,以MS为基本培养基,当6-BA为2.0 mg/L,NAA为0.2 mg/L时,对碧云茶树芽的诱导和增殖最有利,增殖率可达2.84;其中NAA差异不显著,6-BA差异显著。     

老虎须组培实生苗快繁技术及其生根炼苗的移栽效果

为快速繁殖老虎须实生苗应用于实际,采用老虎须种子进行消毒,接种获得无菌实生苗,用叶片和叶柄诱导愈伤组织,进行增殖培养、生根培养和炼苗移栽。结果表明:2%次氯酸钠2 mL和0.1%氯化汞8mL组成的消毒液对老虎须种子消毒灭菌效果最好;温度对老虎须种子发芽率的影响不显著;最好的愈伤诱导培养基为MS+BA 0.2mg/L+NAA 0.1mg/L;MS+BA 3.0mg/L+NAA 0.1mg/L是最适宜的增殖培养基,MS+BA 0.5mg/L+IBA 0.5mg/L是最适宜的生根培养基。草炭∶珍珠岩为1∶1的基质移植练苗较好。     

风箱树组织培养研究

以风箱树(Cephalanthus occidentalis L.)带腋芽的茎段为外植体,探讨不同植物激素(6-BA、IBA、NAA)对试管苗增殖以及植株再生的影响,建立了一套风箱树离体培养技术体系。试验结果表明:启动最适培养基为MS0;增殖最适培养基为MS+6-BA 1.5mg/L+IBA 0.2mg/L;生根最适培养基为1/4MS0;诱导率可达73.3%,增殖系数可达3.55,生根率89.47%,移栽成活率可达到100%。     

“巨峰”葡萄热处理茎尖培养技术研究

以"巨峰"葡萄为试材,经热处理后取其茎尖进行离体培养,通过调整激素的种类和浓度,筛选出了适宜"巨峰"葡萄茎尖分化和生根的培养基;通过对不同移栽基质的研究,筛选出了移栽成活率较高的基质配方。结果表明:38℃热处理时葡萄苗发芽率最高,可达86.67%;以GS为基本培养基,附加BA 0.5mg/L与NAA 0.1mg/L,最适宜于"巨峰"葡萄茎尖分化成苗,分化率可达70.00%;生根培养基以GS+IBA 0.06mg/L的生根率最高。适宜"巨峰"葡萄茎尖组培苗移栽的基质为河沙1份+水苔1份。     

TDZ对杏香兔耳风组培苗复壮的影响

为了了解TDZ对组培苗增殖的影响情况,探讨了TDZ在衰退组培苗复壮过程中的作用,以增殖衰退的杏香兔耳风组培苗为试验材料,就TDZ的不同添加方式(抽滤灭菌和高压灭菌)和不同浓度(0.0、0.2、0.4、0.6、0.8、1.0 mg/L)处理对组培苗增殖系数、有效芽及苗高生长的影响情况进行了试验。结果表明:当TDZ的浓度0.2 mg/L时,TDZ的添加方式对组培苗的增殖系数和有效芽数均有较大影响,抽滤灭菌后的增殖系数和有效芽数要明显高于高压灭菌的;不同浓度的TDZ对有效芽数和髙生长的影响均有极显著的差异性(P0.01),当TDZ浓度为0.8 mg/L时,苗质量总体达到最优,增殖系数为5.11,达到了预期的复壮效果;最优生根培养基配方为1/2MS+NAA 0.02 mg/L+IBA 0.1 mg/L。在杏香兔耳风组培苗复壮过程中,TDZ可作为一种有效的植物生长调节剂使用。