老虎须组培实生苗快繁技术及其生根炼苗的移栽效果

为快速繁殖老虎须实生苗应用于实际,采用老虎须种子进行消毒,接种获得无菌实生苗,用叶片和叶柄诱导愈伤组织,进行增殖培养、生根培养和炼苗移栽。结果表明:2%次氯酸钠2 mL和0.1%氯化汞8mL组成的消毒液对老虎须种子消毒灭菌效果最好;温度对老虎须种子发芽率的影响不显著;最好的愈伤诱导培养基为MS+BA 0.2mg/L+NAA 0.1mg/L;MS+BA 3.0mg/L+NAA 0.1mg/L是最适宜的增殖培养基,MS+BA 0.5mg/L+IBA 0.5mg/L是最适宜的生根培养基。草炭∶珍珠岩为1∶1的基质移植练苗较好。

Rapid Propagation Technique of Tissue Culture Seedlings of Tacca chantrieri and Transplanting of Rooting Seedlings after Adaptation

In order to rapidly propagate T.chantrieri seedlings and apply in actual production, sterile seedlings were gained after seed disinfection and inoculation, calli were induced with leaves and petioles, then enrichment culture, rooting culture and hardening-seedling transplantation were conducted.Results: 2% sodium hypochlorite 2 mL plus 0.1% mercuric chloride 8 mL have the best sterilization effect on T.chantrieri seeds;temperature has no significant influence on seed germination rate;the best callus induction medium is MS+BA 0.2 mg/L+NAA 0.1 mg/L;the optimal enrichment medium is MS+BA3.0 mg/L+NAA 0.1 mg/L, and the optimal rooting medium is MS+BA 0.5 mg/L+IBA 0.5 mg/L, 1∶1 of peat and perlite is suitable for hardening-seedling transplantation.