抗新城疫病毒HN蛋白单抗可变区基因的克隆与序列分析

提取抗新城疫HN蛋白单克隆抗体C3-B7杂交瘤细胞总RNA,进行RT-PCR,扩增出轻重链可变区基因。凝胶回收纯化后与pMD-18T载体连接,重组载体转化于宿主菌DH5α,筛选出阳性重组子,菌液PCR鉴定后进行测序。结果显示,VH基因全长357 bp,编码119个氨基酸;VL基因全长332 bp,编码110个氨基酸。这为单链抗体的构建及表达奠定了基础。     

Influences of aluminum ions on the determination of zpc (zero point of charge) of variable charge soils

Influence of Al dissolution on soil ZPC (zero point of charge) measured by a potentio-metric titration (PT) method and a modified salt titration (STPT) method was examined using two strongly weathered soils from Thailand and two volcanic ash soils from Japan. The amount of dissolved Al ions increased with the increase in the concentration of a supporting electrolyte for the strongly weathered soils, while the increase was negligible for the volcanic ash soils.

ZPC value of the strongly weathered soils determined by the PT method was lower than that by the STPT method, due to the greater Al dissolution associated with the higher electrolyte concentration used in the PT method. Al ions adsorbed onto the soil surface would shift the ZPC to a higher pH value not as a result of the formation of hydroxy Al polymers, but due to the blocking of permanent negative charge sites, which could otherwise lower the ZPC. The σp value, as a measure of permanent charge or the amount of 11 or O11 adsorbed by a soil required to attain the ZPC, could be used to describe this phenomenon.

In the STPT method, the salt concentration was not high enough to causc a significant Al dissolution at the ZPC, which is considered to be a more suitable condition than in the PT method because the ZPC value can be evaluated at a low salt concentration as in the ease of field conditions for crop production. Thus, the STPT method is rccommendcd for the determination of the ZPC.     

Ion Transport Dynamics in Acid Variable Charge Subsoils

This is a mini-review of the research work conducted by the authors with the objective of studying ion transport in variable charge subsoils collected from different areas around the world. An attempt is made in these studies to relate the unique behavior manifested during ionic transport in these subsoils with their mineralogical, physical and chemical properties, which are markedly different from those in soils from temperate regions. The variable charge subsoils have a relatively high salt sorption capacity and anion exchange capacity (AEC) that retards anions downward movement. The AEC correlates closely with the anion retardation coefficients. Ca²⁺ applied with gypsum in topsoil may be transported to the subsoil and may improve the subsoil chemical properties. These results may help in developing appropriate management strategies under a range of mineralogical, physical, and chemical conditions.     

基于逆模型解耦的风力提水变论域模糊控制

针对风力提水系统风、辅动力存在耦合使控制效果难以保证的问题,该文提出了一种基于逆模型解耦的变论域模糊控制方法。采用神经网络方法辨识风力提水系统的逆系统,并与被控对象进行串联,建立伪线性系统,实现风、辅动力解耦。同时采用模糊控制器对风、辅动力分别进行独立控制,对模糊控制器的论域进行改进,增强系统的环境适应能力。逆模型仿真试验中变量泵控制和柴油机控制量平均误差分别为6.3%和4.5%,实际运行中离心泵的转速平均误差控制在5%以内,说明该文方法能有效抑制风速变化的影响。研究结果对风力提水系统的推广具有重要的意义。     

海河流域作物水分敏感系数空间分布

作物水分敏感系数是指导有效灌溉和优化配水的关键参数,研究其空间分布格局对流域水资源优化配置具有重要作用。该文通过空间统计建模,利用探索性空间数据分析技术,分别基于Moran′I统计量、Moran散点图以及空间关联局域指标(local indicators of spatial association,LISA)对作物水分敏感系数(Ky)进行全局、局部空间自相关分析,探索Ky在流域内的空间分布格局。结果表明,Ky在流域空间上呈现出东部平原地区较大,西部及北部山地较小的趋势,其值在0.749～1.668变化。Ky总体上存在显著的空间正相关关系(Moran′I为0.6009～0.6077,且p<0.0001),而且呈现明显的空间集聚特征;高-高集聚区位于北京、天津等东部平原地区,低-低集聚区位于承德、秦皇岛和大同等北部和西部山地,高-高和低-低集聚类型区占据整个流域的80%,其中约有一半表现显著(p<0.05),而低-高和高-低集聚区仅有少数;Ky空间自相关程度随距离的增加而减弱,在距离为240～280km时自相关系数接近于0。总之,Ky形成以东部平原地区为"高敏感核心区",逐渐向西部及北部山地发散并降低的核心-边缘空间分布格局。研究结果可为该地区节水灌溉和水资源优化配置提供指导。     

一类变系数性全时滞系统的稳定性

本文利用ΛЯДУНОВ-РАзумихин定理,对一类变系数线性全时滞系统给出了一致稳定、一致渐近稳定和不稳定的代数判别准则.     

广东省杉木树种二元立木材积可变参数模型研究

采用155株建模样本,利用现代建模理论和方法,以山本式固定参数模型和可变参数模型建立了广东省杉木树种的二元立木材积模型。模型自检及64株检验样木验证表明,固定参数模型和可变参数模型精度均符合建模要求,能满足生产需要,但后者具有更优的适用性能。     

变频调速技术在水泵控制中的应用

介绍水泵变频调速控制系统的节能原理,系统结构及节能效果。     

鼠类机会性寄生虫感染的实验研究

将住家附近诱捕到的74只黄胸鼠、43只褐家鼠和56只小家鼠随机分为实验Ⅱ组和对照组。实验Ⅰ组90只野鼠饮用1.0/L醋酸地塞米松水溶液；实验Ⅱ组66只野鼠给予皮下注射醋酸可的松；对照组不作任何处理。结果从实验Ⅰ组和实验II组的野鼠粪便和各种组织内共分离出5种机会性寄生虫，即微小隐孢子虫，结肠小袋纤毛虫，溶组织内阿米巴，弓形虫和卡氏肺孢子虫。然后将分离的虫体分别制成接种物，接种各种实验动物。接种后.经免疫处理的实验动物均出现典型的临床症状或死亡；未经免疫处理的实验动物则呈隐性感染。结果证明在机体免疫功能低下时.实验动物接种机会性病体后均能获得感染。提示我们，鼠类机会性寄生虫可能成为人体和其他动物感染的传染源，在公共卫生学上有重要意义。