中药穴位注射对奶牛胎衣不下的疗效观察

奶牛产后胎衣不下对奶牛业造成极大的经济损失。本试验旨在研究一种简便易行、成本低、高效的非手术治疗方法。本试验应用当归及复方丹参注射液经百会穴及交巢穴注射,使胎衣排出率达100%,用药后的当年受孕牛占96.2%。可见,此方法可以有效治疗奶牛产后胎衣不下并能提高奶牛的当年受孕率。     

Cryopreservation of carnation (Dianthus caryophyllus L.) shoot tips by encapsulation-vitrification

Shoot tips excised from in vitro cultured plants of Dianthus caryophyllus L. (cv. Pallas, cv. Pink Candy and cv. Wanessa) were successfully cryopreserved using an encapsulation-vitrification method. Shoot tips (2–3 mm in length) were encapsulated in sodium alginate, precultured on liquid Murashige and Skoog (1962) medium supplemented with various sucrose concentrations (0.25, 0.5, 0.75, 1.0 M) for 24 h or 48 h and dehydrated with the vitrification solution PVS2 (up to 4 h) at 24 °C or 0 °C prior to direct immersion in liquid nitrogen (−196 °C). A maximum of shoot regeneration from cryopreserved shoot tips was obtained with the following combinations: preculture in 0.5 M sucrose and 180 min dehydration treatment at 0 °C for cv. Pallas (60% shoot formation), or preculture in 0.75 M and 200 min dehydration at the same temperature for cv. Pink Candy (66.6% shoot formation) and cv. Wanessa (73% shoot formation).     

Pretreatment of hypertonic saline attenuates the hepatic ischemia reperfusion injury induced by neutrophils

AIM: To explore the effect of the pretreatment of hypertonic saline (HTS) in hepatic ischemia reperfusion (I/R) injury.METHODS: The rats were divided into sham group (sham group), ischemia reperfusion group (IR group) and pretreatment of hypertonic saline group (HTS group). Partial hepatic ischemia reperfusion model was used. The rats were sacrificed at the time of 1 h, 3 h, 6 h, 12 h and 24 h after reperfusion in each group, respectively. Blood samples were obtained to examine ALT. The expression of the CD11b/CD18 (Mac-1) on the neutrophils was analyzed by flow cytometry. RT-PCR and Western blotting were used to examine the expression of intercellular adhesion molecule-1 (ICAM-1) in livers and chromatometry was performed to detect the activity of myeloperoxidase (MPO) in livers. The morphology of hepatocytes and the structure of sinusoid were observed by histological examinations. RESULTS: ① HTS pretreatment decreased the level of ALT at the time points of 3 h, 6 h and 12 h after reperfusion (P<0.05). ② Mac-1 expression in HTS group was lower at 6 h and 12 h after reperfusion compared with IR group (P<0.05). ③ MPO activity in HTS group was lower at 6 h, 12 h and 24 h compared with IR group (P<0.05). ④ RT-PCR and Western blotting analysis indicated that the pretreatment of HTS inhibited the expression of ICAM-1 in livers after reperfusion. ⑤ Moderate hepatocyte swelling and few neutrophil infiltration were observed in HTS group.CONCLUSION: Pretreatment with HTS has the effect on hepatic ischemia reperfusion injury by inhibiting the expression of Mac-1 on circulating neutrophils and the expression of ICAM-1 in the liver.     

蘑菇培养料加入864菌液发酵与二次发酵的效果比较

通过加入８６４菌液发酵和二次发酵栽培蘑菇的试验,比较培养料中微生物群落、培养料成份及产菇量,说明加入８６４菌液发酵是代替二次发酵栽培蘑菇的较好方法。     

意大利蜜蜂近距离采蜜量与工蜂日龄的关系

在距蜂箱１０ ｍ 的地点放置质量浓度为０．７５ ｋｇ· Ｌ－ ３的蔗糖液，以测定各日龄意蜂的个体采蜜量．结果表明，采集蜂最小日龄为３ 日龄，１３～２７ 日龄段的工蜂采蜜量最高，每只蜜蜂每次采集６９．４４～７５．２５ ｍ ｇ；１２ 日龄以前的标记蜂采蜜量不稳定，随着日龄的增长逐渐趋于稳定；在测定地点，标记蜂１１ 日龄开始明显增多，直到２８ 日龄后减少．根据实验结果可以初步判定，近距离采集的适龄采蜜蜂为 １３～２７ 日龄．此项研究结果对蜜蜂饲养管理技术的改进有重要参考价值．     

二阶泛函微分方程周期解存在性的重合度方法

利用 Ｍａｗ ｈｉｎ 的重合度理论，研究了二阶泛函微分方程周期解的存在性，并举例说明了其应用⒚     

菊花无土栽培技术研究

采用无土栽培技术，对９０余个品种的２６０余盆菊花进行了营养液配方及基质、栽培方式的试验研究，认为在太原市水质及气候条件下，菊花的无土栽培方式以虹吸式、半液式为宜，营养液配方采用３号配方、１号配方或朱士吾提出的通用配方略加调整。     

输油管道仿真技术及其应用

系统阐述了输油管道仿真软件的基本理论,即数学模型与数值解法.介绍了现代管道仿真技术及其发展趋势,列举了一些目前国际上比较通用的商业软件,给出了开发国内输油管道软件的建议.     

不同硝酸钾溶液浓度对垂直一维入渗特性影响研究

通过室内不同浓度溶液的垂直一维入渗试验,分析了不同浓度碳酸钾溶液影响下的湿润锋和累积入渗量的变化规律以及土壤含水量和硝态氮浓度的分布规律,由不同浓度溶液累积入渗量,依据GREEN-AMPT入渗模型,反推出湿润锋面平均吸力和饱和导水率,结果表明增大溶液浓度提高了垂直一维入渗的饱和导水率,但减小了湿润锋面的平均吸力,溶液浓度通过影响湿润体的饱和导水率和湿润锋面的平均吸力来影响入渗量,二者的综合作用是增大了入渗量。     

最佳线性无偏预测估计种畜育种值（BLUP）可视化计算机程序

应用VB6可视化编程软件，设计较先进的最佳线性无偏预测估计种畜育种值（BLUP法）程序，增强了软件的功能，使其更具易用性、广泛性。