高职高专院校图书馆知识管理实践探索

本文阐述了知识管理与高职高专图书馆知识管理的概念,分析了高职高专院校图书馆知识管理的特征与现状,提出了适合一套适合高职高专院校图书馆实际的知识管理实施策略。     

一种基于数据动态迁移的分级备份系统的研究

随着运营商信息化进程的不断深化，业务数据量处于高速的增长，管理信息系统的高效访问以及安全性正在变得越来越重要。本文以信息系统的数据价值评估为基础，提出一种数据动态迁移模型，并引入虚拟带库作为近线存储，形成完整的解决方案。实现传统备份到分级备份的升级改造，优化信息系统备份架构，提高数据的访问以及保护的效率。     

“微”时代视域中公共图书馆知识服务研究

在信息内容图像化、信息互动性强的"微"时代,公共图书馆仅仅作为信息中心无法满足大众多层次的文化需求。从"微"时代公众阅读特点入手,从知识的发现、知识转变为服务、服务的实现三个方面提出公共图书馆知识服务构想,更好地满足微时代背景下公众信息需求。     

图书馆科技查新工作初探

介绍了科技查新的内涵、原则及工作要求,通过介绍科技查新的工作流程,分析得出科技查新工作质量控制的措施。     

基于总分馆制的县—乡（村）图书馆服务网络构建

介绍了当前我国基层公共图书馆的发展状况,提出了构建基于总分馆制的县—乡（村）图书馆服务网络的构思,并对构建服务网络中的一些问题给出自己的思考。     

超星数字图书馆版权授权模式研究

版权是我国数字图书馆发展的瓶颈，指出超星版权模式是我国数字图书馆行业比较成熟的授权方式，总结了超星版权模式的发展历程，分析了其发展过程中存在的不足，对超星数字图书馆版权模式的发展提出了建设性意见。     

Randomly primed,strand-switching,MinION-based sequencing for the detection and characterization of cultured RNA viruses

RNA viruses rapidly mutate, which can result in increased virulence, increased escape from vaccine protection, and false-negative detection results. Targeted detection methods have a limited ability to detect unknown viruses and often provide insufficient data to detect coinfections or identify antigenic variants. Random, deep sequencing is a method that can more fully detect and characterize RNA viruses and is often coupled with molecular techniques or culture methods for viral enrichment. We tested viral culture coupled with third-generation sequencing for the ability to detect and characterize RNA viruses. Cultures of bovine viral diarrhea virus, canine distemper virus (CDV), epizootic hemorrhagic disease virus, infectious bronchitis virus, 2 influenza A viruses, and porcine respiratory and reproductive syndrome virus were sequenced on the MinION platform using a random, reverse primer in a strand-switching reaction, coupled with PCR-based barcoding. Reads were taxonomically classified and used for reference-based sequence building using a stock personal computer. This method accurately detected and identified complete coding sequence genomes with a minimum of 20× coverage depth for all 7 viruses, including a sample containing 2 viruses. Each lineage-typing region had at least 26× coverage depth for all viruses. Furthermore, analyzing the CDV sample through a pipeline devoid of CDV reference sequences modeled the ability of this protocol to detect unknown viruses. Our results show the ability of this technique to detect and characterize dsRNA, negative- and positive-sense ssRNA, and nonsegmented and segmented RNA viruses.     

人兽共患寄生虫病候选疫苗分子筛选方法研究进展

人兽共患寄生虫种类多、宿主广泛且危害严重。血吸虫病、棘球蚴病、囊尾蚴病、旋毛虫病、弓形虫病等是常见的重要人兽共患寄生虫病。人类和家畜饱受寄生虫病的危害,这对公共卫生和畜牧业造成了很大的影响。控制传染源、切断传播途径和保护易感群是控制人兽共患寄生虫病流行的综合防控措施。在综合防控策略中,疫苗的使用是切断循环链、控制乃至消灭人兽共患寄生虫病的理想和有效途径之一。选用高效的抗原筛选方法挖掘潜在的疫苗候选分子是开发疫苗的前提和关键。抗原筛选技术的更新换代使得研究者发掘出了更多新抗原和保护性多肽。现有的抗原筛选方法主要包括传统的粗抗原筛选法、cDNA文库筛选法、蛋白质组学筛选法、生物信息学及多组学技术联合筛选法。很多抗原筛选的方法是伴随寄生虫疫苗研究的发展应运而生的,粗抗原筛选法是基于抗原抗体相互反应的免疫学原理而设计的,此方法筛选的天然抗原可引起机体较强的免疫反应;cDNA文库筛选抗原的优势在于筛选更有针对性,所以候选产物的成分更单一、明确;蛋白质组学筛选法是基于质谱而兴起的一种筛选技术,它既可对未知蛋白组分进行鉴定,还可对鉴定结果进行差异比较,在未知分子的发现和功能特殊的靶分子筛选中发挥着重要作用;随着后基因时代的到来,生物信息学及多组学联合筛选技术使得抗原筛选逐步进入了多维、立体的筛选模式,也使得候选抗原及其表位的功能研究更加深入,这为基因工程疫苗和多肽疫苗候选分子的筛选提供了技术手段。     

Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential

A recombinant phage library harbouring Mycoplasma meleagridis (MM) genomic DNA fragments was generated in the bacteriophage lambda gt11 expression vector. The library was screened for expression of MM specific antigens with a polyclonal antiserum that had been preadsorbed with antigens of the most common unrelated avian mycoplasma species. A 49-amino acid antigenic domain unique to MM was isolated, expressed in Escherichia coli, and its serodiagnostic potential was demonstrated. An antiserum raised against this MM-specific antigenic domain recognized a cluster of seven membrane-associated MM proteins with molecular masses ranging from 34 to 75 kDa. Overall, this study resulted in the identification of a potent serodiagnostic tool and revealed the complex antigenic nature of MM.     

高校数字图书馆有效服务模式研究

在分析高校数字图书馆有效服务模式、主要特征的基础上,构建了一个以用户为中心的高校数字图书馆这一信息服务模式,并探讨了实现这一有效服务模式的途径与策略。