解磷菌的降解能力及其培养特性的研究

采用限制性培养的方法从大庆多年耕作的农田土壤中分离筛选出的无机磷降解菌1株。在以Ca3(PO4)2为唯一磷源的平板培养中对其解磷能力进行测定,结果能观察到溶磷圈,但溶磷圈直径较小;在液体培养法中利用钼锑抗比色法对其解磷能力进行测定,在以Ca3(PO4)2为唯一磷源的培养液中的可溶性磷得率为10.01%,解磷能力较强。通过研究不同初始pH、温度、气液比对解磷能力的影响,确定分解Ca3(PO4)2的最佳条件为30℃,180r/min,pH7～8。气液比越小越有利于溶磷。     

高CO2气调储藏对大米食用品质调控效应的研究

以W45号大米为试材,定量控制储藏环境中的O2和CO2浓度,研究10℃和30℃条件下,高浓度CO2储藏6个月对大米不溶性直链淀粉含量及蒸煮品质的影响。结果表明:在低温10℃、O2浓度8%条件下,高浓度CO2可有效抑制大米不溶性直链淀粉含量的增加,CO2浓度作用效果20%10%2%,其中20%CO2气调储藏大米的不溶性直链淀粉含量与初始值相比仅增加了9.39%,显著低于对照(不充气处理);在高温30℃、O2浓度8%条件下,高浓度CO2调控大米不溶性直链淀粉的效应不显著;在10℃、30℃温度储藏条件下,高CO2处理均可显著提高大米的蒸煮品质,抑制大米的加热吸水率,增加米汤可溶性固形物含量,CO2浓度越高效果越明显,其中20%CO2处理在10℃条件下加热吸水率比对照下降了12.71%,米汤可溶性固形物含量比对照增加了24.56%。     

杨树解磷细菌蜡状芽孢杆菌和荧光假单胞菌发酵罐扩繁条件优化

对杨树高效解磷细菌蜡状芽孢杆菌(Bacillus cereus)JYZ-SD1和荧光假单胞菌(Pseudomonas fluorescens)JW-JS1在3L发酵罐里发酵条件进行优化。结果显示这两株解磷细菌在72h内都是接种量20%、转速400 r/min、通气量100ln/h条件下菌体生长量最大。为将这两株杨树根际解磷细菌更高效、更大量的扩繁提供参考依据。     

Echinacoside promotes bone regeneration by increasing OPG/RANKL ratio in MC3T3-E1 cells

Echinacoside (ECH), isolated from Cistanche tubulosa (Schrenk) R. Wight stems, was subjected to in vitro experiments to investigate its bioactivities on proliferation, differentiation and mineralization of the osteoblastic cell line MC3T3-E1. MTT assay, the alkaline phosphatase (ALP) activity and calcium deposition were determined, and the secretion of collagen I (COL I), osteocalcin (OCN), osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) were also assayed by enzyme-linked immunosorbent assay (ELISA). The results showed that ECH caused a significant increase in cell proliferation, ALP activity, COL I contents, OCN levels and an enhancement of mineralization in osteoblasts at the concentration range from 0.01 to 10 nmol·L^− 1 (p < 0.05), suggesting that ECH has a stimulatory effect on osteoblastic bone formation or has potential activity against osteoporosis. In addition, the ratio of OPG/RANKL also could be enhanced by ECH. These findings provide the potent evidence that ECH can promote bone regeneration in cultured osteoblastic MC3T3-E1 cells, which might be done by elevating the OPG/RANKL ratio, and potential evidence for echinacoside to be a promising drug or a lead compound in the development of disease-modifying drug to prevent osteoporosis.     

Expression of PDGFR-β and Kit in canine anal sac apocrine gland adenocarcinoma using tissue immunohistochemistry

Canine anal sac apocrine gland adenocarcinoma (ASAGAC) is an uncommon but highly invasive and metastatic malignancy. Toceranib phosphate (Palladia) is a receptor tyrosine kinase (RTK) inhibitor that targets several members of the split kinase RTK family. These membrane receptors are important for cell cycling, apoptosis and angiogenesis, all of which can contribute to carcinogenesis. The objective of this study was to evaluate archived, paraffin-embedded canine ASAGAC and normal canine anal sacs for immunohistochemical detection of Kit and platelet-derived growth factor receptor beta (PDGFR-β). Two of 77 neoplasms (2.6%) expressed Kit. Fifteen of the neoplasms (19.5%) were positive for PDGFR-β expression. None of the normal canine anal sac epithelium expressed Kit or PDGFR-β. Because of these results, further investigation should be considered to determine the role of RTKs in the clinical course and treatment of canine ASAGAC.     

外源性磷酸肌酸停跳液对儿童心肌保护作用的临床观察

目的:观察外源性磷酸肌酸对儿童心肌保护作用。方法:将30例心脏手术患儿随机分为实验组和对照组,每组15例。实验组采用含磷酸肌酸(CP)的st.Thomas 号停跳液,对照组采用st.Thomas 号停跳液,分别以手术中心脏停跳液用量、心脏停搏情况、心脏复跳情况、术后心律失常的发生率、血中肌钙蛋白T(Tn T)评价两种诱导停跳液的心肌保护作用。结果:(1)实验组停跳液能达到与对照组停跳液同样好的停跳效果;(2 )心脏自动复跳率、复跳后ST段改变、术后心律失常的发生率,两组间差异无显著性(P>0 .0 5 ) ;(3)实验组术中、术后6、2 4、4 8h的血Tn T水平均明显低于对照组(P<0 .0 1)。结论:磷酸肌酸停跳液较单纯晶体停跳液具有更好的心肌保护作用。     

复合磷酸盐抑制酸奶中霉菌生长的应用

研究了复合磷酸盐在酸奶中对发酵剂酸化速率和产品品质的影响,以及复合磷酸盐最适添加量抑制霉菌生长的效果。结果表明,复合磷酸盐添加量0.02%和0.04%对发酵剂酸化速率影响不明显,并且对产品品质没有影响,但是对酸奶中霉菌生长具有明显的抑制作用。因此,在酸奶生产中添加适量复合磷酸盐可以有效提升酸奶产品货架期。     

磷酸替米考星对姜曲海品系仔猪的安全性试验研究

为客观评价磷酸替米考星可溶性粉对靶动物猪临床应用的安全性,本试验选取50头40日龄健康断奶姜曲海品系仔猪,分别以1倍、3倍、5倍及10倍剂量10％磷酸替米考星可溶性粉连续饮水给药21 d,喂服受试药物前后受试猪的血常规、血生化、血凝、尿常规、料重比等指标及病理组织学结果表明,受试仔猪没有表现出明显不良症状,除10倍剂量受试猪的血生化指标中血清钙、肌酸激酶与空白对照组相比有明显差异(P＜0.01)外,其他各用药组受试猪的各检测指标与空白对照组没有显著差异或其测定值均位于正常值范围,未出现与用药明显相关的不良状况.结果说明10％磷酸替米考星可溶性粉毒性较小,受试猪体的耐受性高,至少以10倍推荐剂量连续饮水给药21 d对靶动物猪是安全的.本试验对姜曲海品系仔猪进行了血常规、血生化、血凝、尿常规、饲料报酬等方面数据的补充,为其相关指标的进一步研究提供了一定的参考依据.