The aim of this study was to evaluate the ability of nonpathogenic F. oxysporum and Trichoderma isolates from suppressive soils in South Africa to suppress fusarium wilt of banana in the glasshouse. Several biological control agents and commercial biological control products were included in the study. The isolates were first screened in vitro on potato dextrose agar. In glasshouse evaluations, the fungal and bacterial isolates were established on banana roots before they were replanted in pathogen-infested soil, while the commercial biocontrol agents were applied as directed by the supplier. Banana plantlets were evaluated for disease development after 7 weeks. In vitro tests showed none of the nonpathogenic isolates suppressed Fusarium oxysporum f.sp. cubense ( Foc ), while slight suppression was observed with the two Trichoderma isolates. Results of the glasshouse evaluations revealed that two of the nonpathogenic F. oxysporum isolates, CAV 255 and CAV 241, reduced fusarium wilt incidence by 87·4 and 75·0%, respectively. The known biological control agent Fo47 did not suppress Foc significantly. Pseudomonas fluorescens strain WCS 417, known for its ability to suppress other fusarium wilt diseases (WCS 417), reduced disease incidence by 87·4%. These isolates should be further evaluated for potential application in the field, independently and in combination. 相似文献
A healthy soil is often defined as a stable soil system with high levels of biological diversity and activity, internal nutrient
cycling, and resilience to disturbance. This implies that microbial fluctuations after a disturbance would dampen more quickly
in a healthy than in a chronically damaged and biologically impoverished soil. Soil could be disturbed by various processes,
for example addition of a nutrient source, tillage, or drying-rewetting. As a result of any disturbance, the numbers of heterotrophic
bacteria and of individual species start to oscillate, both in time and space. The oscillations appear as moving waves along
the path of a moving nutrient source such as a root tip. The phase and period for different trophic groups and species of
bacteria may be shifted indicating that succession occurs. DGGE, Biolog and FAME analysis of subsequent populations in oscillation
have confirmed that there is a cyclic succession in microbial communities. Microbial diversity oscillates in opposite direction
from oscillations in microbial populations. In a healthy soil, the amplitudes of these oscillations will be small, but the
background levels of microbial diversity and activity are high, so that soil-borne diseases will face more competitors and
antagonists. However, soil-borne pathogens and antagonists alike will fluctuate in time and space as a result of growing plant
roots and other disturbances, and the periods and phases of the oscillations may vary. As a consequence, biological control
by members of a single trophic group or species may never be complete, as pathogens will encounter varying populations of
the biocontrol agent on the root surface. A mixture of different trophic groups may provide more complete biological control
because peaks of different trophic groups occur at subsequent locations along a root. Alternatively, regular addition of soil
organic matter may increase background levels of microbial activity, increase nutrient cycling, lower the concentrations of
easily available nutrient sources, increase microbial diversity, and enhance natural disease suppression. 相似文献
A key element in the EU Common Agricultural Policy (CAP) will be a single farm payment system that is linked to compliance with rules on, for instance hygiene standards. However, there are no recommended methods for assessing the hygiene proficiency of pig production farms. The present study was undertaken to develop a method for this purpose. A first implementation was done on pilot scale; with a set of both conventional and organic pig farms (N = 15). Fifty hygiene-related factors were selected, especially with reference to the possible proliferation of enteric pathogens Listeria monocytogenes, Yersinia enterocolitica and Yersinia pseudotuberculosis. The factors were allocated into 8 evaluation categories: (1) general production management, (2) animal density, (3) the outdoor area for pigs, (4) pest and pet animals, (5) general hygiene in the piggery, (6) pen hygiene, (7) feed production hygiene, and (8) feeding hygiene. A farm questionnaire and a supplementary on-site observation form were devised, and one arbitrary scale of hygiene points assigned for each factor. In addition to the mailed questionnaires, one person subsequently visited all the farms, perused the questionnaire with the owners and completed the observation form. The hygiene scores of the farms in each evaluation category were compared both without weighting and with weighting based on expert opinions. The method proved to be feasible and applicable to different types of production. 相似文献
While the cumulative effects of the actions of multiple owners have long been recognized as critically relevant to efforts
to maintain sustainable forests at the landscape scale, few studies have addressed these effects. We used the HARVEST timber
harvest simulator to predict the cumulative effects of four owner groups (two paper companies, a state forest and non-industrial
private owners) with different management objectives on landscape pattern in an upper Michigan landscape managed primarily
for timber production. We quantified trends in landscape pattern metrics that were linked to Montreal Process indicators of
forest sustainability, and used a simple wildlife habitat model to project habitat trends. Our results showed that most trends
were considered favorable for forest sustainability, but that some were not. The proportion of all age classes and some forest
types moved closer to presettlement conditions. The trend for the size of uneven-aged patches was essentially flat while the
average size of patches of the oldest and youngest age classes increased and the size of patches of the remaining age classes
decreased. Forest fragmentation generally declined, but edge density of age classes increased. Late seral forest habitat increased
while early successional habitat declined. The owners use different management systems that cumulatively produce a diversity
of habitats. Our approach provides a tool to evaluate such cumulative effects on other landscapes owned by multiple owners.
The approach holds promise for helping landowner groups develop and evaluate cooperative strategies to improve landscape patterns
for forest sustainability. 相似文献
There is a growing body of literature showing that physical activity and nature have a positive effect on people's health and well-being. Additionally literature indicates that there may even be a synergic benefit from being physically active whilst simultaneously being directly exposed to nature. This insight is used in recovery programmes for work related stress and mental health care settings. However, as primary care is usually people's first point of contact with the health care system, the greatest benefits for people's health can probably be obtained in primary health care settings. The aim of the present study is therefore to investigate to what extent general practitioners advise patients on physical activity and whether they refer to the additional health benefits of physical activity in a natural environment.
A secondary analysis was performed on a random sample of 2784 video-taped patient consultations of 100 general practitioners in The Netherlands. Many characteristics of these consultations were known on the basis of earlier analyses: diagnosis, age and sex of patient and whether or not the issue of physical activity had been brought up by either patient or doctor. First, we investigated to what extent and with which health problems advice was given concerning physical activity. Second, from these consultations, a stratified sample of 100 consultations was observed in order to investigate whether an advice was given by the general practitioner concerning the additional benefits of nature. In 26% of all consultations advice on physical activity was given by the general practitioner. Physical activity was mentioned more often in cases of vague complaints than in cases with a clear cut diagnosis. As could have been expected, physical activity was mentioned relatively frequently in cases of musculoskeletal complaints and complaints of the nervous system. The additional benefits of physical activity in natural environments was not mentioned during any of the consultations.
In conclusion, general practitioners regularly give advice on physical activity. However, they fail to mention that physical activity in nature areas might have additional health benefits. Given the fact that medical treatment is increasingly evidence based, we think the evidence for the health benefits of physical activity in nature needs to be stronger. Results from such research could eventually find their way into the guidelines of the general practitioners. 相似文献
AIM: To investigate the effect of advanced glycation end products on inflammation in cultured cardiomyocytes.METHODS: Primary cardiomyocytes were isolated from Sprague-Dawley neonatal (1 to 2 days old) rats ventricles.The insulin resistant cardiomyocyte model was established.Neonatal rat ventricular myocytes were exposed to AGEs for 24 hours.TNF-α mRNA and PPAR-γ mRNA expressions were determined by RT-PCR.Activation of NF-κB in the cells was examined by using immunocytochemistry.The ultrastructure of the cells was detected by transmission electron microscope.RESULTS: The exprssion of TNF-α mRNA and the activation of NF-κB increased,the expression of PPAR-γ mRNA decreased compared with control group (P<0.05).The differences among different AGE-BSA groups were significant (P<0.05).The numbers of chondriosome and smooth endoplasmic reticulum increased.CONCLUSION: AGEs significantly increase TNF-α mRNA expression and NF-κB activation,and restrain the expression of PPAR-γ mRNA.These data suggest that AGEs play an important role in the onset of diabetic cardiomyopathy. 相似文献
An analysis of records of plant pathogens first identified in Great Britain from 1970 to 2004 (inclusive) was undertaken to determine the numbers of new species that have become established over time. Results show that the numbers of newly recorded pathogens have not varied significantly. Of the 234 pathogens recorded for the first time between 1970 and 2004, 157 were fungi, 27 were oomycetes, 26 were viruses, 23 were bacteria, and one was a phytoplasma. Approximately 53% of pathogens were found on ornamental crops, 16% on horticultural crops, 15% on wild native species, 12% on agricultural crops, 2% on pasture plants and 2% on exotic forestry tree species. Where the origin of introductions was known or strongly suspected, 47% came from the Netherlands. About 38% of newly recorded pathogens with information on the location of first record were discovered in the South East region of England. Plant Pathologists regarded 19% of all new pathogens as important because of actual or potential economic/environmental losses. The results indicate that the numbers of new or important pathogens establishing in recent years are not increasing and that most new findings are associated with ornamental plants. 相似文献