豫西丘陵地区果牧结合立体生态园设计的探讨

根据豫西丘陵地区生态、经济、社会条件以及目前农牧业生产中存在的突出问题，提出果牧结合立体生态园建设是该地区生态农业发展的重要途径。并在调查和初步研究基础上设计了“畜（禽）—果”、“畜（禽）—沼—果”等果牧结合模式，分析了生态园建设研究中要探讨的具体内容。     

地温空调井抽回灌效果影响关键因子识别

为了探析引起地温空调井抽回灌效果的主要制约因素,通过单相关系数分析、主成分绝对得分的源解析模型、拟合度检验和多元线性回归的方法分析影响单井回灌效率的影响因素。结果表明:井径、滤水管孔隙率及抽回灌井数比与单井回灌效率的相关性和拟合度较好,故将井径、滤水管孔隙率及抽回灌井数比作为影响单井回灌效率的关键因子,同时分析了单井回灌效率与关键因子的之间的拟合方程及多元线性回归方程,得到了单井回灌效率与关键因子之间的定量关系。     

Construction and Identification of Yeast Surface Display Libraries of Ovis aries DRA Gene Exon 2

The specific primers were designed according to Ovis aries DRA gene sequence deposited in GenBank and the multiple cloning site of the plasmid pYD1,which was a vector used for protein surface display on Saccharomyces cerevisiae.The gene encoding DRA was amplified by PCR using the genomic RNA of Ovis aries.The 762 bp fragment was cloned and released in GenBank and registration number was KR422362.The PCR product was inserted into the yeast surface display plasmid vector pYD1 by double enzyme digestion.It was indicated that DRA gene was successfully integrated into the genome.Dot mutation was made at both ends of exon 2 in DRA gene for making restriction enzyme cutting site and design the exon 2 specific primers according to mutated Ovis aries DRA gene sequence.Sequenced exon 2 amplification products based on DNA pooling of sheep large sample template was analyzed the polymorphic loci.The polymorphic exon 2 246 bp fragment was obtained by double enzyme digestion and connected to surface display restructuring mutation carriers pYD1-DRA by the same double enzyme digestion,and then we successfully constructed yeast surface display libraries.We transformed it into Saccharomyces cerevisiae EBY100 cell.Yeast monoclone was identified by PCR amplification and sequencing,and we confirmed that DRA gene had been integrated into Saccharomyces cerevisiae genome.After galactose induced,it was detected that DRA gene library had been successfully demonstrated on the yeast cell surface under the fluorescence microscope by immunofluorescence method.     

响应面法优化杜仲叶提取物的制备工艺

优化杜仲叶提取物制备的工艺条件,在单因素试验基础上,以杜仲叶提取物浸膏得率和绿原酸转移率为评价指标,采用Box-Behnken中心组合设计法优化杜仲叶提取物制备的工艺条件。杜仲叶提取物制备的最佳工艺条件为乙醇浓度500mL/L,液料比10∶1(mL/g),提取温度97℃,提取4次,每次30min;在此最佳工艺条件下,杜仲叶提取物浸膏得率和绿原酸转移率综合评分均值为99.08,与预测值基本一致。响应面法(RSM)优化的杜仲叶提取物制备工艺稳定可靠。     

Effects of water‐misting sprays with forced ventilation on post mortem glycolysis,AMP‐activated protein kinase and meat quality of broilers after transport during summer

Effects of water‐misting sprays with forced ventilation on post mortem glycolysis, adenosine monophosphate‐activated protein kinase (AMPK) and meat quality of broilers after transport during summer were investigated in the present paper. A total of 105 mixed‐sex Arbor Acres broilers were divided into three treatment groups: (i) 45 min transport without rest (T); (ii) 45 min transport with 1 h rest (TR); and (iii) 45 min transport with 15 min water‐misting sprays with forced ventilation and 45 min rest (TWFR). Each treatment consisted of five replicates with seven birds each. The results indicated that the water‐misting sprays with forced ventilation could mitigate the stress caused by transport under high temperature conditions during summer, which reduced the energy depletion in post mortem Pectoralis major (PM) muscle. This resulted in a higher energy status compared to the T group, which would decrease the expression of phosphorylation of AMPK (p‐AMPK). Furthermore, decreased the expression of p‐AMPK then slowed down the rate of glycolysis in post mortem PM muscle during the early post mortem period, which in turn lessened the negative effects caused by transport on meat quality. In conclusion, water‐misting sprays with forced ventilation may be a better method to control the incidence of the pale, soft and exudative meat in broilers.     

外包钢加固法的安全性防范措施研究

采用外包角钢加固的钢筋混凝土柱,其加固效果和安全性与诸多因素,如加固设计、加固施工工艺、加固端锚固构造、加固角钢的增钢率与增箍率、原柱所处的应力状态、使用过程中的维护管理等有关。对外包钢加固钢筋混凝土柱过程中影响其加固效果和安全性的诸多因素的安全性防范措施进行了初步的分析研究和探讨。     

河西绿洲灌区留茬覆盖免耕保护性耕作的增产节水效应

试验设20 cm留茬压倒(NPS20),40 cm留茬压倒(NPS40),40 cm立秆(NS40),20 cm立秆(NS20),6 750kg/hm2覆盖(NSB40),3 750 kg/hm2覆盖(NSB20)和传统耕作(CT)7个处理,在节水1 950 m3/hm2的条件下,研究了不同的秸秆覆盖量、留茬高度和秸秆处理对小麦产量、水分利用效率(WUE)和不同时期土壤水分的影响。结果表明,留茬覆盖免耕保护性耕作提高了春小麦的产量,NPS20,NPS40,NS40,NS20,NSB40和NSB20较CT分别增产53.08%,46.59%,40.81%,19.93%,17.33%和4.34%;留茬覆盖免耕保护性耕作提高了春小麦的水分利用效率(WUE)(NPS20,NS40,NPS40,NS20,NSB40,NSB20分别较CT提高58.02%,43.40%,47.27%,23.78%,20.69%,8.56%),但增加了小麦生育期土壤水分支出;不同处理在不同时期对土壤水分的影响不同。