黑麂血液生理生化指标的测定

首次检测了黑麂血液常规和生理生化等23项指标,并与鹿科梅花鹿做了比较。测定结果显示,黑麂的红细胞、血小板、血红蛋白、红细胞比容高于梅花鹿指标,而白细胞、红细胞体积、红细胞平均血红蛋白浓度、平均血红蛋白量、红细胞体积分布宽度指标低于梅花鹿指标。黑麂白细胞分类结果显示,白细胞和单核细胞约占90%;嗜酸性粒细胞和嗜碱性粒细胞所占百分比各占约2%。黑麂血清生化指标测定结果显示,总胆固醇和血糖两项指标高于梅花鹿的指标;甘油三酯(TG)及极低密度脂蛋白(VLDI-C)含量显著高于梅花鹿。检测结果反映了黑麂的机体生理机能,是进行疾病临床诊断和治疗的依据。     

GM-CSF induces human vascular endothelial cells to form vessel-like structure and the role of VEGF

AIM: To investigate the influence of GM-CSF on human vascular endothelial cells induced to form new blood vessels and the role of VEGF. METHODS: HUVECs were cultured by Matrigel to set up a stable angiogenesis system with the stimulating factors. The rhGM-CSF concentration-dependent and time-dependent effects and the role of VEGF165 were detected. CD34 was measured by immunochemical staining and numbers of vessel formation was calculated under microscopic observation. RESULTS: After treatment with rhGM-CSF at various concentrations and at different time points, the numbers of vessel formation increased in a dose-dependent and time-dependent manner. In the presence of VEGF165, the numbers of vessel formation increased evidently. CONCLUSION: HUVECs were induced to develop tubular structure in vitro cultured with Matrigel. GM-CSF promotes human vascular endothelial cells to form vessel-like structure in vitro in a dose-dependent and time-dependent manner. VEGF also in vitro promotes human vascular endothelial cells to form new vessel-like structure.     

静松灵硬膜外腔注射对绵羊的镇静镇痛作用及其血液变化的研究

在１２只绵羊按０．３ｍｇ／ｋｇ剂量硬膜外腔注射静松灵,观测了镇静镇痛作用的效应,并对血液气体和酸碱平衡状态的变化进行了定量分析,结果表明静松灵硬膜外腔注射的镇静镇痛效果明显,显效时间短,持续时间达１２０ｍｉｎ,对体温,脉搏,呼吸与吸气量有显著作用（Ｐ〈０．０５）,对血液中氧分压,氧含量,氧饱和度,二氧化碳分压,剩余碱及碳酸氢盐的影响明显（Ｐ〈０．０５）,而对血液ｐＨ值和血红蛋白含量无显著影响。     

鸡血型系统对马立克氏病肿瘤形成的影响

采用 Ａ、 Ｂ、 Ｃ、 Ｄ 血型系统的１０ 种血型因子试剂测定了亚发雏鸡的血型， 并对马立克氏病肿瘤病变与各血型因子的关系进行了研究。结果表明， 在１４ 日龄感染雏鸡的３０ ～６０ 天内， Ｍ Ｄ Ｖ 对各个器官肿瘤病变形成上， 不同的血型系统因子对其影响有很大的差异， 其中带有 Ａ３０８ 、 Ｂ６９１ 因子的雏鸡对 Ｍ Ｄ Ｖ 较易感， 而带有 Ｂ３６５ 因子的雏鸡抗性较强。同样实验还表明， 采用鸡的（ Ｍ Ｈ Ｃ） Ｂ Ｆ Ｉ Ｖ 血型分型试剂与 Ａ、 Ｂ、 Ｃ、 Ｄ 血型试剂检测人工感染 Ｍ Ｄ Ｖ６０ 天后存活鸡表明： Ｂ３６５ 与 Ｂ２１ 均１００ ％ 存在于这些鸡中， 说明二者抗 Ｍ Ｄ 能力有一定的相似性； 说明 Ａ、 Ｂ、 Ｃ、 Ｄ 血型因子可做为选择标记用于抗 Ｍ Ｄ 选育。     

我国出口观赏鸟血液原虫的调查与分析

通过显微镜检查对来源于全国各地的37种出口观赏鸟进行了血液原虫调查。共检查血样1144份，结果为：血液原虫的检出率为2．1％，主要是血变原虫和／或疟原虫。感染季节以秋冬两季为主，易感鸟品种主要是白腰文鸟、红喉歌鸲、蓝歌鸲、暗绿绣眼鸟、豆冠、白燕、情侣鹦鹉。     

Inducible nitric oxide synthase and arterial pressure regulation

AIM and METHODS:To clarify the role of inducible nitric oxide synthase (iNOS) in the regulation of blood pressure,in the present study, we examined the effect of aminoguanidine (AG), a selective inhibitor of iNOS on the hemodinamical response of Dahl salt-sensitive (DS) and Dahl salt-resistant (DR) rats to low (0.3%) or high (8%) sodium chloride (NaCl) infusion by chronical in vivo hemodynamic experiment, and the effect of NaCl or NaCl plus AG infusion on urinary nitrate (NO₃)/nitrite (NO₂), the end product of nitric oxide (NO), excretion by Greiss Reaction. Furthermore, NOS activity assay was also carried out to probe the effect of NaCl and AG on calcium-dependent or independent NOS activity in renal tissue. RESULTS:1. High or low NaCl-infused DR rats and low NaCl-infused DS rats have no hemodinamical response to AG, however, the hypertensive effect of high NaCl (8%) infusion on DS rats were greatly amplified by co-infusion of AG. 2. Administration of high NaCl significantly elevated the iNOS activity of renal tissue, and greatly increased urinary NO₃/NO₂ excretion. CONCLUSION:Inducible NOS is an important modulator of arterial pressure, especially in case of higher blood pressure.     

日粮阴阳离子差对泌乳前期热应激奶牛血液酸碱平衡和生产性能的影响

研究日粮阴阳离子差对泌乳前期热应激奶牛血液酸碱平衡和生产性能的影响,选用36头热应激条件下泌乳前期荷斯坦奶牛,采用完全随机试验设计(n=12),以不同日粮阴阳离子差[DCAD;(Na K )-(Cl- S2-)mEq/kgDM]为试验处理,即对照组(DCAD为 130.49 mEq/kg DM),试验Ⅰ组(DCAD为 251.54 mEq/kg DM)和试验Ⅱ组(DCAD为 383.87 mEq/kg DM),试验期42 d。结果表明:(1)提高日粮DCAD可明显增强奶牛血液的缓冲能力和运氧能力。血液pH和碱超值随着日粮DCAD的升高而上升(P<0.05),DCAD对其他血气指标的影响不明显,其中二氧化碳总量、二氧化碳分压、实际碳酸氢盐、氧饱和度均逐渐增加(P>0.05),而氧分压则呈峰形变化(P>0.05);(2)DCAD对奶牛血清尿素氮、总蛋白、白蛋白的影响均达到了极显著水平(P<0.01),肌酸磷酸激酶变化明显(P<0.05),血糖含量直线上升(P>0.05);(3)适当提高日粮DCAD可显著提高奶牛的生产性能。试验Ⅰ组和试验Ⅱ组的4%标准乳较对照组分别提高了11.0%和5.1%(P<0.05),而乳脂率明显降低(P<0.05),乳蛋白率(P<0.05)和乳糖含量(P>0.05)随日粮DCAD的升高而增加。综合以上各项指标考虑,建议以试验Ⅰ组(DCAD为 251.54 mEq/kg DM)作为泌乳早期奶牛的DCAD日粮。     

Clinical use of tissue plasminogen activator for systemic thrombolysis in dogs and cats

IntroductionSystemic administration of tissue plasminogen activator (tPA) is seldomly reported in dogs and cats.AnimalsClient-owned animals receiving tPA (2010–2020).Materials and methodsMedical records of dogs and cats receiving tPA for distant known/suspected thrombus were reviewed. Fourteen dog visits (24 injections) and five cat visits (six injections) were included.ResultsCanine known/suspected thrombus included pulmonary thromboembolism (n=6), intracardiac thrombus (n=4), aortic thrombus (n=1), cranial vena cava thrombus (n=2), and femoral and iliac veins thrombus (n=1). Various canine primary diseases were represented, but open-heart surgery was the most common cause. Median time between diagnosis/suspicion of thrombus and tPA injection was 24.5 h (range, 3–150 h). Mean total tPA dose was 1.0±0.78 mg/kg.Clinical improvement occurred in 93% of dogs. Non-fatal complications were reported in 14% of dogs. Dogs’ survival to discharge was 78.6% without identifiable non-survivor characteristics. Feline known/suspected thrombus included unilateral feline aortic thromboembolism (FATE) (n=2), bilateral FATE (n=2), and right renal artery thrombus. Feline primary diseases included cardiomyopathy (n=5). Median time between diagnosis/suspicion of thrombus and tPA injection was 4 h (range, 2–17 h) and median total tPA dose was 1.0 mg/kg (range, 0.6–1.4 mg/kg).Clinical improvement occurred during 40% of the visits. All cats (n=3) with acute kidney injury (AKI) at admission developed worsening AKI and reperfusion injury. Of the remaining two visits, one developed a non-fatal AKI. Cats’ survival to discharge was 40%.ConclusionsSystemic thrombolysis with tPA seems to be effective and safe in dogs. More investigation is needed in cats.